NIssui日水代理 05918 RPM1640培養(yǎng)基2 RPM1640 Medium ② 100g
NISSUI日水培養(yǎng)基
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代理優(yōu)勢：貨期2-3周。
NIssui日水 RPM1640培養(yǎng)基
| Code | Formn | Package | Storage | Shelf?Life |
| 05918 | Powder | 100?g | Cool | 1?Year |
Directions
Dissolve?10.2?g?of?the?medium?in?distilled?waterand?adjust?the?volume?to?1,000?mL.?Sterilize?byautoclaving?at?121?*C?for?15?minutes.?Cool?the sterilized?medium?to?room?temperature?and?add?aproper?amount?of?sterile?10%?sodium?bicarbonate (pH?will?be?7.1?–?7.4?at?37*C,?in?an?atmosphere?of5%?co,?in?air?by?adding?18?–?30?mL?of?sodiumbicarbonate?solution.)?Aseptically?add?0.3?g?Lglutamine?and?the?desire?amount?or?serum.
Summary
RPMI?1640?is?a?medium?for?suspension?cultureinvestigated?by?George?E.?Moore,?former?directorof?Roswell?Park?Memorial?Institute.?Up?to?now,such?media?were?not?autoclavable?because?ofdecomposition?or?deterioration?of?the?constituents,?so?that?sterilization?had?been performed? by?filtration?using?a?membrane?filter.RPMI?1640?Medium?“Nissui”?2?is?an?autoclavable-powdered?medium?specially?manufactured?toovercome?the?difficulties?associated?with?filter-sterilized?media,?such?as?contamination?by Mycoplasma.?This?medium?has?growth?promotingeffect?to?primary?cultures?of?mouse?and?humanleukemia?cells,?various?kinds?of?cell?lines?andKATO-Iil?cells.
Storage?and?Expiration
Close?tightly?and?store?at?25*C?in?a?dark?and?dryplace.?May?be?stored?up?to?one?(1)?year.
Precautions
Do?not?use?the?product?after?its?expiry?date.
Quality?of?the?product?is?not?warranted?after?itsshelf?life.
When?if?medium?or?reagent?touched?eyes?ormouth,?immediately?wash?with?plenty?of?water,and?consult?a?physician.
Any?medium,?reagent?and?materials?must?be
sterilized?by?autoclaving?or?boiling?water?after?use,and?then?disposed?as?industrial?waste?according?tothe?law?or?regulations?related?to?dispose.
