日水 (Nissui) 快速測試皿使用說明書

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日水 (Nissui) 快速測試皿使用說明書

NISSUI制藥公司提供全套食品和環(huán)境衛(wèi)生檢測產(chǎn)品，適合于食品相關機構、醫(yī)院、藥品制造企業(yè)、實驗室及其他藥品相關機構。 用于食品衛(wèi)生檢測、環(huán)境控制、食品毒性檢測、水中大腸桿菌檢測、食品過敏性檢測、食品生產(chǎn)環(huán)境的清潔檢測、菌落計數(shù)及控制系統(tǒng)。

本品用于微生物計數(shù)及檢測，簡易性相當于3M公司的Petrifilm 微生物檢測紙片，但其有具有平板的性質(zhì)，檢測靈敏性準確性優(yōu)于Petrifilm

原理：一旦液體樣品滴入，樣品就會自動均勻擴 散入平板，被包被固體培養(yǎng)基轉化成凝膠

特性：
小而致密的固體培養(yǎng)基（已滅菌）
樣品可在平板中自動均勻擴散
基于顯色底物的清晰顯色
與常規(guī)方法（混合稀釋培養(yǎng)）有較好的相 關性
可挑取群落且非常容易
可常溫保存，保質(zhì)期在一年以上

使用：
A. Compact Dry “Nissui” TC（菌落總數(shù)計數(shù)板）
性質(zhì)：該培養(yǎng)基由非選擇性的培養(yǎng)基和氧化還原指示劑氧化三苯基四氮唑（TTC）組成，多數(shù)菌落生長在TC上顯紅色
步驟：
1、將樣品稀釋
2、將稀釋樣品加到培養(yǎng)基上
3、培養(yǎng)48小時
4、讀取所有菌落

B. Compact Dry “Nissui”CF （大腸菌群計數(shù)板）
性質(zhì)：大腸菌類會在培養(yǎng)基上顯現(xiàn)藍或藍綠色的菌落，指示劑為X-GAL，除大腸菌類外的細菌被抑制，即使生長也不能形成帶色菌落
步驟：
1、將樣品稀釋
2、將稀釋樣品加到培養(yǎng)基上
3、培養(yǎng)24小時
4、讀取所有藍和藍綠色菌落

C. Compact Dry “Nissui”EC（大腸桿菌及大腸菌群計數(shù)板）
性質(zhì)：培養(yǎng)基含有兩種產(chǎn)色的酶底物品紅-GAL和X-GLUC，大腸桿菌顯藍或藍綠色，除大腸桿菌外的大腸菌類顯紅或粉紅色
步驟：
1、將樣品稀釋
2、將稀釋樣品加到培養(yǎng)基上
3、培養(yǎng)48小時
4、讀取所有藍和藍綠色菌落即為大腸桿菌，讀取所有紅藍菌落總和即為大腸菌類的總數(shù)

D. Compact Dry “Nissui”SA（金黃色葡萄球菌計數(shù)板）
性質(zhì)：金黃色葡萄球菌產(chǎn)生黃色素顯現(xiàn)亮黃色菌落，并且用它的酶分解蛋黃的脂質(zhì)-蛋白質(zhì)復合物，該酶將菌落周圍的外緣培養(yǎng)基變成渾濁（蛋黃反應）
步驟：
1、將樣品稀釋在
2、將稀釋樣品加到培養(yǎng)基上
3、滴入蛋黃懸浮液
4、培養(yǎng)48小時
5、讀取外緣培養(yǎng)基為白色渾濁的菌落

E. Compact Dry “Nissui”VP（腸道弧菌計數(shù)板）
性質(zhì)：parahaemolyticus 弧菌產(chǎn)生藍或藍綠色菌落，產(chǎn)物中含有一種特殊的產(chǎn)色物質(zhì)，其他的弧菌形成白色或無色的菌落
步驟：
1、將樣品稀釋在
2、將稀釋樣品加到培養(yǎng)基上
3、培養(yǎng)過夜
4、讀取藍或藍綠色菌落即為parahaemolyticus 弧菌

F. Compact Dry “Nissui”YM（酵母及霉菌計數(shù)板）
性質(zhì)：培養(yǎng)基中含有X-PHOS，使得多數(shù)酵母菌顯藍色，一部分顯奶油色，抗生素抑制細菌生長，霉菌形成帶特征色的毛狀菌落
步驟：
1、將樣品稀釋
2、將稀釋樣品加到培養(yǎng)基上
3、培養(yǎng)3-7天
4、讀取藍至奶油色菌落即為酵母菌，帶特征色的毛狀菌落為霉菌

G. Compact Dry “Nissui”SL（沙門氏菌計數(shù)板）
性質(zhì)：樣品中的沙門氏菌由三種不同的實驗原理共同分辨，通過沙門氏菌賴氨酸脫羧作用，培養(yǎng)基堿化，培養(yǎng)基由藍紫色變?yōu)辄S色，產(chǎn)色物質(zhì)作用，菌落又變成綠色，隨著氫硫化物的產(chǎn)生，菌落邊黑色。

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Nissui Compact Dry 培養(yǎng)板

貨號? 品名? 用途? 包裝? 保質(zhì)期（1-30℃）
06740
Compact Dry TC?
 菌落總數(shù)計數(shù)板?
40片?
18個月
06741
240片?
06744?
Compact Dry CF?
 大腸菌群計數(shù)板?
40片?
06745?
240片?
06742?
Compact Dry EC?
 大腸桿菌及大腸菌群計數(shù)板?
40片?
06743?
240片?
06734?
Compact Dry SA?
 金黃色葡萄球菌計數(shù)板?
40片?
12個月
06735?
240片?
06748?
Compact Dry VP?
 腸道弧菌計數(shù)板?
40片?
06749?
240片?
06746?
Compact Dry YM?
 酵母及霉菌計數(shù)板?
40片?
06747?
240片?
06732?
Compact Dry SL?
 沙門氏菌計數(shù)板?
40片?
06733
240片?

日水Nissui培養(yǎng)基的用途：可廣泛用于活體菌總體計數(shù)、細胞培養(yǎng)、環(huán)境檢測及食品衛(wèi)生等。日水Nissui培養(yǎng)基的優(yōu)勢：日水Nissui培養(yǎng)基質(zhì)量優(yōu)越，技術領先，不但突破一般品牌無法高溫滅菌的限制，特別在細胞培養(yǎng)的后期傳代體現(xiàn)出其他品牌無法替代的優(yōu)點。日水Nissui培養(yǎng)基的保證：所有培養(yǎng)基生產(chǎn)原料均由純化學品經(jīng)嚴格控制在無菌條件下制成，絕不含任何動物性的原料，是疫苗生產(chǎn)和細胞培養(yǎng)等生物制藥的佳選擇。

1.?Food?Hygienic?Test?(Dry?Plate?Media):

Code Product?Name Application
06732 Compact?Dry?SL?(40?plates) Salmonella?detection
06733 Compact?Dry?SL?(240?plates) Salmonella?detection
06734 Compact?Dry?SA?(40?plates) ?Staphylococcus?aureus
06735 ?Compact?Dry?SA?(240?plates) Staphylococcus?aureus
06738 Swab?kit?“Nissui”?Easy?swab?kit Designed?for?Compact?Dry
06740 Compact?Dry?TC?(40?plates) ?Total?viable?count
06741 Compact?Dry?TC?(240?plates) Total?viable?count
06742 Compact?Dry?EC?(40?plates) E.?coli?/?Coliforms
06743 ?Compact?Dry?EC?(240?plates) E.?coli?/?Coliforms
06744 ?Compact?Dry?CF?(40?plates) Coliforms
06745 ?Compact?Dry?CF?(240?plates) Coliforms
06746 Compact?Dry?YM?(40?plates) Yeast?and?Mold
06747 Compact?Dry?YM?(240?plates) Yeast?and?Mold
06748 Compact?Dry?VP?(40?plates) Vibrio?parahaemolyticus
06749 Compact?Dry?VP?(240?plates) Vibrio?parahaemolyticus

2.?Food?Hygienic?Test?(Stamp?Method):

 

Code Product?Name Application
06050 ?Food?Stamp?Standard?Method?Agar?(100plates) Total?viable?count
06051 Food?Stamp?Standard?Method?Agar?(30?plates) Total?viable?count
06054 Food?Stamp?TCBS?Agar?(100?plates) Vibrio?parahaemolyticus
06055 Food?Stamp?TCBS?Agar?(30?plates) Vibrio?parahaemolyticus
06056 Food?Stamp?TGSE?Agar?(100?plates) Staphylococcus?aureus
06057 Food?Stamp?TGSE?Agar?(30?plates) Staphylococcus?aureus
06063 Food?Stamp?Sabouraud?Agar?(100?plates) Fungi
06064 Food?Stamp?Sabouraud?Agar?(30?plates) Fungi
06750 Food?Stamp?MLCB?Agar?(100?plates) Salmonella
06751 Food?Stamp?MLCB?Agar?(30?plates) Salmonella
06752 Food?Stamp?Cereus?Agar?(100?plates) Bacillus?cereus
06753 Food?Stamp?Cereus?Agar?(30?plates) Bacillus?cereus
06754 Food?Stamp?Potato?dextrose?Agar?with?CP?(100plates) Food?poisoning?Fungi
06755 Food?Stamp?Potato?dextrose?Agar?with?CP?(30plates) Food?poisoning?Fungi
06763 ?Food?Stamp?X-GAL?Agar?(100?plates) Coliforms
06764 Food?Stamp?X-GAL?Agar?(30?plates) Coliforms
06775 Food?Stamp?XM-G?Agar?(100?plates) ?E.?coli?/?Coliforms
06776 Food?Stamp?XM-G?Agar?(30?plates) E.?coli?/?Coliforms

3.?Food?Hygienic?Test?(EVANET?EV20?/?EVATEST)

 

Code Product?Name Application
01700 EVATEST?SET?(30?tests) Staphylococcal?Enterotoxin
01702 EVATEST?VT?1/2?(30?tests) EHEC?Verotoxin
01300 EVANET?EV20?(1?unit) Automated?Fluorescent?Immunoassay?System

4.?Environmental?Test?(for?cleanliness?check)

Code Product?Name Application
09516 Lucifelight?(50?det.?X?4) For?cleanliness?check?used?ATP?detection?method
09518 Lucifelight?Wiping?Kit?(200?tubes) For?cleanliness?check?used?ATP?detection?method
09534 Lucifelight?LF-100?(1?unit) Instrument?for?Lucifelight

 

5.?Environmental?Test?(Stamp?Method)

 

Code Product?Name Application
For?Medical?Environment
06780 Clean?Stamp?MSO?Agar?(100?plates) For?MRSA
06781 Clean?Stamp?MSO?Agar?(30?plates) For?MRSA
06782 Clean?Stamp?MSEY?Agar?(100?plates) For?Staphylococcus
06783 Clean?Stamp?MSEY?Agar?(30?plates) For?Staphylococcus
For?Regular?Environment
06784 ?Clean?Stamp?SCD?Agar?(100?plates) For?Total?viable?count
06785 Clean?Stamp?SCD?Agar?(30?plates) For?Total?viable?count
06786 Clean?Stamp?SCDLP?Agar?(100?plates) For?Total?viable?count?containing?inactivator
06787 Clean?Stamp?SCDLP?Agar?(30?plates) For?Total?viable?count?containing?inactivator
06790 Clean?Stamp25?SCD?Agar?(100?plates) For?Total?viable?count
06791 Clean?Stamp25?SCD?Agar?(30?plates) For?Total?viable?count
06792 Clean?Stamp25?SCDLP?Agar?(100?plates) For?Total?viable?count?containing?inactivator
06793 Clean?Stamp25?SCDLP?Agar?(30?plates) For?Total?viable?count?containing?inactivator
06794 Clean?Stamp25?CPSB?Agar?(100?plates) For?Fungi
06795 Clean?Stamp25?CPSB?Agar?(30?plates) For?Fungi

6.?E.?coli?/?Coliforms?detection?from?Water

 

Code Product?Name Application
05608 EC?Blue?50P?(100?packs?for?50mL) For?E.?coli?/?Coliforms?detection?medium?from?Water
05611 EC?Blue?50?(100?bottles?for?50mL) For?E.?coli?/?Coliforms?detection?medium?from?Water
05613 EC?Blue?10?(150?bottles?for?10mL) For?MPN?(minimum?probable?number)

7.?Food?Allergen?Detection?Kit

 

Code Product?Name Application
08600 FASTKIT?ELISA?“Egg”?Kit For?Food?Allergen?screening?(Egg)
08601 FASTKIT?ELISA?“Milk”?Kit For?Food?Allergen?screening?(Milk)
08602 FASTKIT?ELISA?“Wheat”?Kit For?Food?Allergen?screening?(Wheat)
08603 ?FASTKIT?ELISA?“Buckwheat” For?Food?Allergen?screening?(Buckwheat)
08604 FASTKIT?ELISA?“Peanut” For?Food?Allergen?screening?(Peanut)

8.?Colony?counting

 

Code Product?Name Application
01504 NeQCS?(1unit) For?automated?colony?counting

9.?Culture?Media?(dehydrated)

 

Code Product?Name Application
For?Salmonella
05002 ?EEM?Broth?(100g) Enrichment?broth?for?Salmonella
05020 SS?Agar?(20L) Isolation?of?Salmonella
05021 SS?Agar?(4L) Isolation?of?Salmonella
05032 SS?Agar?with?Sucrose?(280g) Isolation?of?Salmonella
05033 SS?Agar?with?Sucrose?(20L) Isolation?of?Salmonella
05131 Buffered?Peptone?Water?(BPW)?(300g) ?Enrichment?broth?for?Salmonella
For?Staphylococcus
05234 Staphylococcus?Medium?No.?110?(300g) Isolation?of?Staphylococcus
05236 Mannitol?Salt?Agar?(300g) Isolation?of?Staphylococcus
For?E.?coli?/?Coliforms
05036 MacConkey?Agar?(300g) Isolation?of?Enteric?bacteria
05607 ?Blue?Light?Broth?(300g) Rapid?detection?of?E.?coli?and?Coliforms?from?Water?and?Foods
05639 ?Lauryl?Sulfate?MUG?Broth?(300g) Rapid?detection?of?E.?coli?and?Coliforms?from?Water?and?Foods
05642 X-GAL?Agar?(300g) Chromogenic?enzyme?substrate?agar?for?Coliform?isolation
05643 MacConkey?Sorbitol?Agar?(300g) Isolation?for?E.?coli?O157
05647 XM-G?Agar?(300g) ?Chromogenic?enzyme?substrate?agar?for?E.?coli?and?Coliforms
For?Bacillus?cereus
05282 NGKG?Agar?Base?(300g) Selective?isolation?of?B.?cereus?from?Foods
For?Enterococci
05679 EF?Agar?Base?(100g) Isolation?of?Enterococci?from?Water,?Milk?and?Meat?products
For?general?use
05503 Heart?Infusion?Agar?(300g) Isolation?for?many?kind?of?bacteria
05505 Heart?Infusion?Broth?(100g) Isolation?for?many?kind?of?bacteria
05506 Brain?Heart?Infusion?Agar?(300g) Isolation?for?many?kind?of?bacteria
05508 Brain?Heart?Infusion?Broth?(300g) Isolation?for?many?kind?of?bacteria
05516 Trypto-Soya?Agar?(SCD?Agar)?(300g) ?Isolation?for?many?kind?of?bacteria
05533 Mueller-Hinton?Agar-N?(300g) Isolation?for?many?kind?of?bacteria
For?Yeast?and?Mold
05701 Sabouraud?Agar?(300g) Isolation?of?Yeast?and?Mold
05703 Candida?GE?Agar?(100g) Isolation?for?Candida
For?Anaerobes
05426 GAM?Agar,?Modified?(300g) Isolation?for?Anaerobic?bacteria
05433 GAM?Broth,?Modified?(100g) Isolation?for?Anaerobic?bacteria
05440 Bacteroides?Agar?(100g) Differentiation?and?isolation?for?Bacteroides
05441 FM?Agar?(100g) Differentiation?and?isolation?for?Fusobacterium
06593 Anaero?Mate-P?(50?sets?for?100?plates) Simple?bag?for?Anaerobic?culture
06594 Anaero?Mate-J?(50?sets?for?500?plates) Simple?bag?for?Anaerobic?culture

10.?Identification?Test?Kit

Code Product?Name Application
06626 ID?Test?EB-20?(25?tests) Identification?test?for?Enterobacteriaceae
06629 ID?Test?NF-18?(25?tests) Identification?test?for?Gram?Negative?Non-fermenters
06637 ID?Test?SP-18?(25?tests) Identification?test?for?Staphylococcus