Wako 291-59701 膳食纖維試劑盒,Dietary Fiber Assay Kit

英文名稱：Dietary Fiber Assay Kit

中文名稱：膳食纖維試劑盒

品牌：wako；

貨號：291-59701
包裝：100 tests

類別：糖苷

我司所銷售的化學(xué)試劑、原料等所有產(chǎn)品（包括但不限于抗生素類、蛋白質(zhì)類、試劑盒類產(chǎn)品等）僅限用于科學(xué)研究用途，不得作用于人體。

本產(chǎn)品僅供研究用。不要把它給人類使用。

普洛斯基方法的一個簡單的修正版本。與傳統(tǒng)的Prosky方法相比，該方法具有較高的測量精度。此外，縮短了酶處理時間。

試劑盒組成：

1. 1. 耐熱α-淀粉酶溶液1小瓶x 20 mL

1. 1. 蛋白酶溶液1小瓶x20 mL

1. 1. 酰胺葡萄糖苷酶溶液1小瓶x20 mL
2. 硅藻土1小瓶x100克

測量輪廓

1. 1. 在MES/TRIS緩沖液中溶解的樣品

1. 1. 耐熱α-淀粉酶消化

1. 1. 蛋白酶和淀粉糖苷酶的消化

1. 在pH6.3和60℃下持續(xù)30分鐘
2. 1. 乙醇沉淀

1. 休假1小時
2. 1. 過濾

1. 1. 乙醇和丙酮洗滌

1. 1. 一夜干

1. 1. Kjeldahl蛋白測定及灰分測定

1. 總膳食纖維測定

原理

1. 1. 膳食纖維總量的測定

1. 樣品中的可消化組分，如淀粉和蛋白質(zhì)，會被水解。

1. 用α-淀粉酶、蛋白酶和淀粉糖苷酶，通過添加四倍體積的95%乙醇沉淀未分解的殘余大分子，然后用含硅藻土的玻璃過濾坩堝捕獲。
2. 總膳食纖維含量是通過從玻璃過濾坩堝上捕獲的殘渣的干重中扣除蛋白質(zhì)和灰分的含量來確定的。
3. 1. 可溶性膳食纖維和不溶性膳食纖維含量的分?jǐn)?shù)分析

1. 樣品中的可消化組分，如淀粉和蛋白質(zhì)，用α-淀粉酶、蛋白酶和淀粉糖苷酶水解，然后用含硅藻土的玻璃過濾坩堝過濾。
2. 不溶性膳食纖維含量是通過在玻璃過濾坩堝上從不溶性殘渣的干重中扣除蛋白質(zhì)和灰分的含量來確定的。
3. 同時，在濾液中加入四倍體積的95%乙醇，沉淀未分解的可溶性大分子，用含硅藻土的玻璃過濾坩堝捕集?？扇苄陨攀忱w維含量是通過從玻璃過濾坩堝上沉淀殘渣的干重中扣除蛋白質(zhì)和灰分的含量來確定的。
4. 總膳食纖維含量定義為不溶性膳食纖維含量和可溶性膳食纖維含量之和。
5. 1. 低分子可溶性膳食纖維的測定

1. 1. 用高效液相色譜法測定了特定的低分子可溶性膳食纖維，即使加入四倍體積的95%乙醇也不會沉淀。
   2. 用α-淀粉酶、蛋白酶和淀粉葡萄糖苷酶水解樣品中的淀粉和蛋白質(zhì)等可消化組分，再加入四倍體積的95%乙醇沉淀未分解的殘余大分子。然后用含硅藻土的玻璃過濾坩堝過濾。
   3. 利用離子交換樹脂去除濾液中的蛋白質(zhì)、有機酸和無機鹽，并將其應(yīng)用于高效液相色譜。低分子可溶性膳食纖維含量是由樣品的峰面積與葡萄糖的峰面積(內(nèi)標(biāo))在色譜圖上的比值來確定的。
   4. 同時，通過在玻璃過濾坩堝上從沉淀殘渣的干重中扣除蛋白質(zhì)和灰分的含量，確定了不溶性和可溶性大分子膳食纖維的含量。

總膳食纖維含量定義為低分子可溶性膳食纖維含量和不溶性和可溶性大分子膳食纖維含量之和。

This product is for research use only. Do not administer it to human.

A simple and modified version of Prosky’s method. Compared to conventional Prosky’s method, it assures high measurement accuracy. Furthermore, enzyme treatment time is shortened.

Kit Contents

1. 1. Thermostable alpha-Amylase Solution 1 vial x 20 mL

1. 1. Protease Solution 1 vial x 20 mL

1. 1. Amyloglucosidase Solution 1 vial x 20 mL
2. Diatomaceous Earth 1 vial x 100 g

Measurement Outline

1. 1. Sample dissolved in MES/Tris buffer

1. 1. Digestion with thermostable alpha-amylase

1. 1. Digestion with protease and amyloglucosidase
2. for 30 minutes at pH 6.3 and 60 degrees C
3. 1. Ethanol Precipitation
4. Leave 1 hour
5. 1. Filtration

1. 1. Wash with ethanol and acetone

1. 1. Dry overnight

1. 1. Kjeldahl Protein Determination and Ash Determination

1. Total Dietary Fiber Determination

Principle

1. 1. 1. Determination of total Dietary Fiber Contents
   2. Digestible components in samples, such as starch and protein, are hydrolyzed
   3. with alpha-amylase, protease, and amyloglucosidase, precipitate undecomposed residual macromolecule by adding quadruple volume 95% Ethanol, and then trapped with a glass filter crucible containing diatomaceous earth.
   4. Total Dietary Fiber Contents are determined by deducting weight of protein and ash content from the dry weight of trapped residue on a glass filter crucible.
   5. 1. Fractional Analysis of Soluble Dietary Fiber Contents and Insoluble Dietary Fiber Contents
   6. Digestible components in samples, such as starch and protein, are hydrolyzed with alpha-amylase, protease, and amyloglucosidase, then filtrated with a glass filter crucible containing diatomaceous earth.
   7. Insoluble Dietary Fiber Contents are determined by deducting weight of protein and ash content from the dry weight of trapped insoluble residue on a glass filter crucible.
   8. Meanwhile, add a quadruple volume 95% Ethanol into the filtrate, precipitate undecomposed residual soluble macromolecule, and trap with a glass filter crucible containing diatomaceous earth. Soluble Dietary Fiber Contents are determined by deducting weight of protein and ash content from the dry weight of the trapped precipitated residue on a glass filter crucible.
   9. Total Dietary Fiber Contents are defined as the sum of Insoluble Dietary Fiber Contents and Soluble Dietary Fiber Contents.
   10. 1. Determination of Low-molecular Soluble Dietary Fiber
   11. Specific Low-molecular Soluble Dietary Fibers, which are not precipitated even by addition of a quadruple volume of 95% Ethanol, are determined by high-performance liquid chromatography.
   12. After digestible components such as starch and protein in samples are hydrolyzed with alpha-amylase, protease, and amyloglucosidase, the undecomposed residual macromolecule are precipitated by adding a quadruple volume of 95% Ethanol. Then filter it with a glass filter crucible containing diatomaceous earth.
   13. Proteins, organic acids and inorganic salts in the filtrate are removed with ion-exchange resin, and then the solution is applied to high-performance liquid chromatography. Low-molecular Soluble Dietary Fiber Content is determined by the ratio of the peak area of the sample with that of glucose (internal standard) on the chromatogram.
   14. Meanwhile, Insoluble and Soluble Macromolecule Dietary Fiber Content is determined by deducting weight of protein and ash content from the dry weight of the trapped precipitated residue on a glass filter crucible.

Total Dietary Fiber Contents is defined as sum of Low-molecular Soluble Dietary Fiber Contents and Insoluble and Soluble Macromolecular Dietary Fiber Contents.