德國 Applichem A7089 DNA-ExitusPlus 核酸污染清除試劑

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德國 Applichem A7089 DNA-ExitusPlus 核酸污染清除試劑

AppliChem品牌試劑代理-上海金畔生物科技有限公司
? ? ? ?上海金畔生物代理AppliChem品牌試劑，歡迎新老客戶訪問AppliChem官網(wǎng)或者咨詢我們獲取相關(guān)產(chǎn)品信息。
? ? ? 德國艾普力（APPLICHEM）代理

Product Code:??A7089

Product Name:?DNA-ExitusPlus™

Specifications:

  • removes DNA and RNA contaminations from surfaces
    ? for cleaning of PCR work stations and equipment
    ? for cleaning of electrophoresis equipment, pipetts, reaction tubes etc.
    ? delivered as spray bottles or as refill bottles (RF)
    ♦ The special features
    ? non-enzymatic degradation of DNA and RNA by catalytic and cooperative effects of the components
    ? All components of DNA-ExitusPlus™ are readily biologically degradable and not harmful or toxic for humans.
    ? Doesn’t contain aggressive mineralic acids or alkaline substances
    ? No toxic fumes. Contains low concentration of alcohol.
    Functionality (Strand breakage test): passes test
    Efficacy (ExitusPlus™ activity test): passes test

Comment:

DNA amplification is one of the most commonly used techniques in the modern research laboratory. The presence of contaminating DNA in and around PCR workstations can lead to unwanted artifacts during amplification. Principally, there are two ways to make DNA not amplifiable:1. by degradation of the DNA (e. g. by the addition of DNases or chemical destruction), or2. by modification of bases – leaving the DNA strand intact, but blocked for reading by polymerases. Using a DNA strand break assay (designed by multiBIND biotec GmbH, Germany), it has been shown that not all DNA decontamination solutions on the market totally degrade DNA! DNA-ExitusPlus™ is an improvement over those products and causes both strand breakages as well as degradation. When used properly at the work area, it totally eliminates the amplification of non-target DNA. DNA-ExitusPlus™ is a non-alkaline, non-corrosive and non-carcinogenic cleansing solution which is effective on all surfaces.A severe disadvantage of conventional decontamination reagents is revealed in a new test for the corrosive potential of their components. For this purpose, different metal plates were incubated for 20 minutes with identical aliquots of the reagents. The selected metals are representative for equipment and materials found in laboratories. The result of this corrosion test show that all known commercial products contain aggressive chemical substances with corrosive, harmful or even toxic effects. These conventional reagents are known to contain azides, mineralic acids like phosphoric acid or hydrochloric acid, aggressive peroxides or strong alkaline substances like sodium hydroxide. Even after an incubation of only 20 minutes, irreversible damages of the metal surfaces are observed in many cases. The newly developed solution DNA-ExitusPlus™ exhibits its unique characteristics especially in this corrosion test. For all metal surfaces tested, no damage or corrosion is observed after treatment. DNA-ExitusPlus™ was also tested under identical conditions on many different plastic surfaces without any indications of damage.DNA-ExitusPlus™ is a registered trademark of AppliChem GmbH.

Bibliography:

(1)Esser, K.-H. et al. (2006) Nature Methods 3(2), 151DNA decontamination: DNA-ExitusPlus in comparison with conventional reagents.

Storage:?RT???protected from light

Nature Methods推薦PCR污染清除方法——德國PanReac AppliChem DNA-ExitusPlus
貨號Cat.No.: A7089,0100(100ml)?? ??A7089,0500(500ml)?? ? ?A7089,1000(1L)聚合酶鏈?zhǔn)椒磻?yīng)（Polymerase Chain Reaction）簡稱PCR，是一種選擇性體外擴(kuò)增核酸片段的方法，經(jīng)過循環(huán)復(fù)制，擴(kuò)增產(chǎn)量是指數(shù)上升，所以具有極高的靈敏度，被廣泛應(yīng)用于生物和醫(yī)學(xué)的科研，臨床各種研究中，是目前應(yīng)用最為廣泛的分子生物學(xué)方法之一。
但也正是由于PCR具有極高的靈敏度，微量的核酸污染便會(huì)導(dǎo)致假陽性擴(kuò)增結(jié)果而導(dǎo)致實(shí)驗(yàn)結(jié)果不準(zhǔn)確，這也是廣大實(shí)驗(yàn)者最頭痛的問題，如果形成氣溶膠污染擴(kuò)散，則可引起整個(gè)PCR實(shí)驗(yàn)室的污染，處理起來非常棘手，嚴(yán)重的甚至要關(guān)閉實(shí)驗(yàn)室。環(huán)境中的核酸是主要的污染源，常見的污染源有：
1. 模板提取時(shí)真空抽干裝置
2. 超凈臺(tái)
3. 電泳裝置
4. 紫外分析儀
5. 剪刀、鑷子、刀片、移液器等器械
6. 離心機(jī)
7. 冰箱門把手，冷凍架，門把手或?qū)嶒?yàn)臺(tái)面等
8. 氣溶膠
9. 高壓滅菌裝置

傳統(tǒng)的PCR污染清除方法主要有：稀酸處理法、紫外照射法、尿嘧啶糖苷酶（UNG）法、酶解變性、酒精沉淀等幾種方式，但仍然存在無法徹底破壞DNA分子、試劑具有腐蝕性或者毒性、小片段核酸無法清除等不足。

德國AppliChem獨(dú)家研發(fā)的DNA-ExitusPlus是一種無腐蝕、無致癌性的核酸污染清除溶液，該產(chǎn)品可將裸露的核酸完全降解至無法作為擴(kuò)增模板的小片段，無法被擴(kuò)增，且無法恢復(fù)，從而徹底消除了PCR過程中由于環(huán)境污染而導(dǎo)致的假陽性擴(kuò)增。

DNA-ExitusPlusTM特點(diǎn)：
1. 產(chǎn)品為非酶類試劑；
2. 產(chǎn)品中各組分協(xié)同作用，快速、非序列特異性的降解核酸污染；
3. 所有組分可生物降解、無毒無害；
4. 不含有機(jī)溶劑或者揮發(fā)性物質(zhì)，不含無機(jī)酸或者堿性物質(zhì)，不會(huì)對金屬形成腐蝕；
5. 使用簡單，噴灑或浸泡處理15分鐘即可完成清除作用；
6. 升溫至50度可提高反應(yīng)速度。