Physical Description:??Solid
Product Code:?A2132
Product Name:??Aprotinin BioChemica
Headline Comment:
? registered trademark of Bayer AG
Specifications:
Assay (protein): min. 90 %
Activity (KIU/mg): min. 5500
pH (1 %; H2O): 5.0 – 7.5
Water (K.F.): max. 6 %
Comment:
Aprotinin was isolated independently from two laboratories and originally named Bovine Pancreatic Trypsin Inhibitor?(BPTI) and Trypsin-kallikrein Inhibitor?(TKI). It is a strongly basic protein (58 amino acids; ref. 1, 2). Aprotinin inhibits trypsin, chymotrypsin, kallikrein from different sources and plasmin. Its recommended working concentration is 2 – 10 μg/ml (approx. 1 μM). The working concentration given in ref. 3 (500 KIU/ml; approx. 10 μM) seems to be very high.Stability\: Aprotinin may be stored indefinite at \+4°C in lyophilized form. Dissolved in saline or buffer solutions (e. g. 10 mg/ml) at pH 5 – 8, it may be stored aliquoted at \+4°C at least for one month or frozen at -20°C for several years. Thawed aliquots of the stock solutions should not be frozen again, because repeated freeze-thawing will lead to aggregation of the polypeptide aprotinin. Solutions with a pH value below 4 or above 9 should be used immediately. Protect from direct sunlight, UV light and reducing agents. It is stable in water, 70 % methanol, 70 % ethanol or 50 % acetone, too (2, 3).Unit-Definition: One Trypsin Inhibitor Unit (TIU) will decrease the activity of 2 trypsin units by 50 %, where one trypsin unit will hydrolyze 1.0 μmole Nα-benzoyl-DL-arginine p-nitroanilide (BAPNA) per minute at pH 7.8 and 25°C. 1 TIU (Trypsin Inhibitor Unit) corresponds to approx. 1300 KIU (Kallikrein Inhibitor Units) and 1 TIU corresponds to one Ph. Eur. Unit.
Bibliography:
(1)Kassell, B. & Laskowski, Sr., M. (1965) Biochem. Biophys. Res. Com. 20,?463-468.The basic Trypsin inhibitor of bovine pancreas\: V. The disulfide linkages. (2)Kassell, B. (1970) Methods Enzymol. 19, 844-852Bovine Trypsin-Kallikrein inhibitor (Kunitz Inhibitor, Basic Pancreatic Trypsin Inhibitor, Polyvalent Inhibitor from Bovine Organs). (3)Fritz, H. & Wunderer, G. (1983) Drug Res. 33, 479-494Biochemistry and application of Aprotinin, the Kallikrein inhibitor from bovine organs.
Storage:??2-8°C
規(guī)格：10mg
貨號：A2132_0010
廠商：AppliChem
產品介紹:
Storage: 2-8°C
LGK: 10 – 13
Formula C284H432N84O79S7
M 6511.52 g/mol
CAS-No.: 9087-70-1
HS-No.: 35040090
origin from bovine lung
Specification
Assay (Protein) min. 90 %
Activity (KIU/mg) min. 5500
pH (1 %; H2O) 5.0 – 7.5
Water (K.F.) max. 6 %
Aprotinin was isolated independently from two laboratories and originally named Bovine Pancreatic Trypsin Inhibitor (BPTI) and Trypsin-kallikrein Inhibitor (TKI). It is a strongly basic protein (58 amino acids; ref. 1, 2). Aprotinin inhibits trypsin, chymotrypsin, kallikrein from different sources and plasmin. Its recommended working concentration is 2 – 10 μg/ml (approx. 1 μM). The working concentration given in ref. 3 (500 KIU/ml; approx. 10 μM) seems to be very high.
AppliChem? A2132_0010?Aprotinin BioChemica?10mg?543
AppliChem? A2132_0025?Aprotinin BioChemica?25mg?? 1117
AppliChem? A2132_0100?Aprotinin BioChemica?100mg? 3936