SUMICHIRAL OA系列色譜柱的選擇與使用保養(yǎng)

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SUMICHIRAL OA系列色譜柱的選擇與使用保養(yǎng)

SUMICHIRAL 選擇選擇方法

  • SUMICHIRAL OA-2000 series
  • SUMICHIRAL OA-3000 series
  • SUMICHIRAL OA-4000 series
  • SUMICHIRAL OA-5000 series
  • SUMICHIRAL OA-6000 series
  • SUMICHIRAL OA-7000 series
  • SUMICHIRAL OA-8000 series
  • SUMICHIRAL AGP , CBH , HSA
  • SUMICHIRAL Columns Product list

SUMICHIRAL OA series is a pioneer of chiral HPLC stationary phase using low molecular weight chiral selector.

SUMICHIRAL OA 系列手性柱是高性能的分離光學(xué)異構(gòu)體的HPLC色譜柱。SUMICHIRAL OA色譜柱能直接分離各種異構(gòu)體，并可從非對應(yīng)異構(gòu)體中分離異構(gòu)體，這些非對應(yīng)異構(gòu)體中可能存在氫鍵，電荷轉(zhuǎn)移及主客體等相互作用。OA系列手性柱能精確測定各種手性化合物的純度，如藥物、農(nóng)藥、香料中手性結(jié)構(gòu)的組成。
OA-2000系列柱，改進刷型（Amide），不對稱碳原子鍵合CONH基團
OA-2000系列柱可直接分離芳香類、酯類、羧酸類和醇類化合物的異構(gòu)體。如OA-2000色譜柱分離合成除蟲菊酯，OA-2500可分離布洛芬藥物。
OA-3000系列柱，改性刷型（尿素），不對稱碳原子鍵合NHCONH基團
OA-3000系列色譜柱可有效分離羧酸類化合物，尤其是乙?；途郯滨サ陌被峄衔?。OA-3300色譜柱能直接分離布洛芬藥物，乙酰胺基酸，BOC-氨基酸和芐基氨基酸。
OA-4000系列柱，改進刷型，胺和氨基酸兩個手性中心鍵合NHCONH基團
OA-4000系列柱通過電荷轉(zhuǎn)移和氫鍵相互作用達到手性識別，能分離各種各樣的化合物，如胺類和氨基醇的藥物，醇類，酯類和酰胺類化合物可用正相模式分離。尤其是酰胺和聚氨酯的衍生物，醇類等柱效高。
OA-5000和OA-6000系列柱在反相模式中通過配位交換相互作用進行手性識別。OA-5000手性配位體是青霉胺涂敷在ODS硅膠表面，OA-6000手性配位體是酒石酸衍生物涂敷在ODS硅膠表面，限制了添加到流動相的有機溶劑用量，且OA-5000和OA-6000系列柱的流動相中必須包含Cu2+離子。該系列柱不但能有效的分離氨基酸，羥基酸的對映異構(gòu)體，而且能分離銅螯合形成的化合物，如氨基醇，胺，羧酸，氨基內(nèi)酰胺和二肽。尤其是OA-5000柱應(yīng)用極為廣泛，OA-6000能有效分離β-氨基酸，β-羥基酸和親水性阿明酸。
OA-7000柱手性固定相是β-環(huán)糊精通過新型的空間模式鍵合硅膠?？稍诜聪鄺l件下分離大量的消旋體，包括酮，胺，氨基酸衍生物。
OA-8000柱手性固定相是冠醚手性鍵合氨丙基硅膠，可有效的分離胺，氨基醇和氨基酸的對映異構(gòu)體，尤其是疏水性胺。

Features

  • Enables direct high quality separation of optical isomers.

    Enables direct high quality separation of optical isomers by a large number of theoretical plate.

  • Elution order inversion

    In the SUMICHIRAL OA series, low molecular weight optically active compounds are immobilized onto the stationary phase. By immobilizing an antipode of this optically active component, the elution order of the target substance can be reversed. (Excluding OA-4800/4900/7000 series, 8000, AGP, CBH, and HSA)

  • Applicable for LC-MS

    SUMICHIRAL OA-2500/3000 series is applicable for LC-MS as the recommended mobile phase is methanol containing ammonium acetate.

  • Supercritical Fluid Chromatography (SFC)

    SUMICHIRAL OA-2000 and OA-4000 series are also available for Supercritical fluid chromatography (SFC).

Product lineup

Separation of enantiomers takes place due to the minor energy difference between the solute and the stationary phase caused by diastereomeric interactions (e.g., hydrogen bonding, charge transfer, coordinate bonding).
SUMICHIRAL column types, optically active component, separation mode and target substances are summarized in the table below.

Optical isomers separation columns (low molecular weight isomers)

Amide-type

SUMICHIRAL Opticall acitive components Separation mode Target substances Columns for elution order inversion SUMICHIRAL OA
OA-2000 (R)-Phenylglycine Normal phese Esters, alcohols, amides, ketones OA-2000S
OA-2000I Esters, ketones OA-2000SI
OA-2500 (R)-1-Naphtyl glycine Reversed phese* Carboxylic acids, alcohols, esters, ketones OA-2500S
OA-2500I Normal phese Esters, alcohols, ketones OA-2500SI
Reversed phese* : non-aqueous reversed phase (ammonium acetate/methanol solution)
  • Click here for product details, separation examples, and product list of the SUMICHIRAL OA-2000 series.

Urea-type

SUMICHIRAL Opticall acitive components Separation mode Target substances Columns for elution order inversion SUMICHIRAL OA
OA-3100 (S)-Valine Reversed phase* Carboxylic acids
(N-amino acid derivatives)		 OA-3100R
OA-3200 (S)-tert-Leucine OA-3200R
OA-3300 (R)-Phenylglycine OA-3300S
OA-4000 (S)-Valine, (S)-NEA Normal phese Amines, amides, aminoalcohols, alcohols, esters, phosphamides OA-4000R
OA-4100 (S)-Valine, (R)-NEA OA-4100R
OA-4400 (S)-Proline, (S)-NEA OA-4400R
OA-4500 (S)-Proline, (R)-NEA OA-4500R
OA-4600 (S)-tert-Leucine,
(S)-NEA		 OA-4600R
OA-4700 (S)-tert-Leucine,
(R)-NEA		 OA-4700R
OA-4800 (S)-Indoline-2-carboxylic acid,
(S)-NEA		 　—
OA-4900 (S)-Indoline-3-carboxylic acid,
(R)-NEA		 　—
Reversed phese* : non-aqueous reversed phase (ammonium acetate/methanol solution)
(S)-NEA ： (S)-1- (α-Naphtyl) ethylamine
(R)-NEA ： (R)-1- (α-Naphtyl) ethylamine
  • Click here for product details, separation examples, and product list of the SUMICHIRAL OA-3000 series.
  • Click here for product details, separation examples, and product list of the SUMICHIRAL OA-4000 series.

Ligand exchange-type

SUMICHIRAL Opticall acitive components Separation mode Target substances Columns for elution order inversion SUMICHIRAL OA
OA-5000 (D)-Penicillamine Reversed phase Amino acids, hydroxy acids, amino alcohols, amines OA-5000L
OA-6000 (L)-Tartaric acid,
(R)-NEA		 OA-6000R
OA-6100 (L)-Tartaric acid,
(S)-Valine, (S)-NEA		 OA-6100R
(S)-NEA ： (S)-1- (α-Naphtyl) ethylamine
(R)-NEA ： (R)-1- (α-Naphtyl) ethylamine
  • Click here for product details, separation examples, and product list of the SUMICHIRAL OA-5000 series.
  • Click here for product details, separation examples, and product list of the SUMICHIRAL OA-6000 series.

Host-guest-type

SUMICHIRAL Opticall acitive components Separation mode Target substances
OA-7000 β-Cyclodexitrin Reversed phase Flavonoid derivatives, acids, neutral compunds
OA-7100 β-Cyclodexitrin Acids, neutral compounds
OA-7500 Methyl-β-cyclodexitrin Alcohols, esters
OA-7600 Methyl-α-cyclodexitrin Neutral compounds
OA-7700 Acetyl-β-cyclodexitrin Amines
OA-8000 Chiral pseudo-crown ether Normal phase Primary amines
Reversed phase

 

  • Click here for product details, separation examples, and price table of the SUMICHIRAL OA-7000 series.
  • Click here for product details, separation examples, and price table of the SUMICHIRAL OA-8000 series.

Optical isomer separation columns (proteins)

SUMICHIRAL Opticall acitive components Separation mode Target substances
AGP α1-acid glycoprotein Reversed phase Acids, bases, neutral compounds, amides, ketones
CBH Cellobiohydrolase Reversed phase Bases
HSA Human serum albumin Reversed phase Acids

 

  • Click here for product details, separation examples, and prioduct list of the SUMICHIRAL AGP, CBH and HSA.