Sephadex G-25 in PD-10 Desalting Columns
PD-10 Desalting Columns contain Sephadex G-25 resin for rapid buffer exchange, desalting, and removal of small contaminants (salts, dyes, radioactive labels) from samples using gravity flow or centrifugation.
PD-10 Desalting Columns contain Sephadex G-25 resin for rapid buffer exchange, desalting, and removal of small contaminants (salts, dyes, radioactive labels) from samples using gravity flow or centrifugation.
- Flexible run protocols?– perform desalting and buffer exchange using gravity flow (1.0 to 2.5 mL samples) or centrifugation (1.75 to 2.5 mL samples)
- Flexible throughput?– desalt one sample or multiple samples in parallel
- Good recovery?– in the range of 70% to > 95%
- High desalting capacity?– remove > 98% salt with gravity; > 90% with centrifugation
- Minimal dilution?– typically none when used as spin columns; 1.4× when used with gravity flow
- Time savings compared to dialysis?– inexpensive and convenient alternative to lengthy and troublesome dialysis procedures
What comes in the PD-10 column package?
Each product pack includes 30 columns, a buffer tray, a column stand, and 4 adapters for centrifugation.
Versatile for use with centrifugation or gravity flow
The product instructions include two protocols. The gravity protocol allows simple processing of samples using one or several columns in parallel, without the need for a purification system. The spin protocol enables runs in parallel in a standard centrifuge with minimal dilution of the eluted sample.
Successful use of these prepacked desalting columns
The following publications demonstrate the utility of PD-10 columns:
- desalting of membrane proteins?after anion exchange chromatography
- desalting of variants?of an essential reductase involved in fatty acid biosynthesis
- buffer exchange of an affinity-tagged?acyl carrier protein
Accessories for PD-10 Desalting Columns
A?LabMate buffer reservoir?can be used for one-step application of the column wash and equilibration buffer using the gravity flow protocol. It is not included with this product but can be purchased separately.
Other Sephadex G-25 columns from GE
GE Healthcare’s Life Sciences solutions for desalting include a wide range of columns containing Sephadex G-25 resin. Options are available to accommodate sample volumes from 100 μL to 15 mL and use with manual methods or a chromatography system. Let GE help you?find the right desalting product.
GE 17-0851-01 Disposable PD-10脫鹽層析柱的詳細(xì)介紹
GE 17-0851-01 Disposable PD-10脫鹽層析柱PD-10脫鹽層析柱與一個方便的直立層析柱架一起提供。使用LabMate緩沖液儲液器使柱平衡過程更容易進行。
?? 快速、高回收率的脫鹽和緩沖液置換，也可用于除小分子化合物；預(yù)裝Sephadex G-25填料。
?? 操作靈活，可用離心或重力流。
?? 容易使用，便于平衡、上樣、洗脫。
?? 重力流方式樣品量1-2.5ml；離心方式樣品量1.75-2.5ml。平行處理多個待脫鹽樣品，可除去90%以上鹽離子。
?? 蛋白回收率在70%-95%。
?? LabMate Buffer Reservoir使得平衡PD-10層析柱更容易，更方便。
PD-10層析柱提供了一個經(jīng)濟、方便的選擇，可取代冗長、繁瑣的透析過程。幾分鐘內(nèi)，完成生物樣品脫鹽、小分子可溶物的去除或替換，同時有極好的回收率。
Disposable PD-10 Desalting Columns經(jīng)過改進設(shè)計，能應(yīng)用于兩種新操作方式：重力流和離心，更為靈活方便。重力流操作可選用一到數(shù)根柱子，平行操作多個樣品進行除雜處理，而無需純化設(shè)備。離心操作則能以標(biāo)準(zhǔn)的離心程序平行處理洗脫樣品，將樣品稀釋降低到最少。
每個包裝內(nèi)含有四個接頭，方便連接配置。與LabMate Buffer Reservoir（需另行購買）聯(lián)合使用進行重力流操作時，清洗和平衡用的緩沖液能在同一步中加入。
如需要設(shè)計經(jīng)濟適用，通用性好的制備樣品工藝，推薦購買PD-10 columns和批量包裝的填料。
Disposable PD-10脫鹽預(yù)裝層析柱每盒有30根。每根柱均保存在含有0.15% Kathon CG保護劑的蒸餾水中。顆粒大小為20-85μm的填料填充在兩個燒結(jié)的聚乙烯熔塊中。熔塊保護填料免于在重力緩沖液流下而干柱。層析柱的出口用可重復(fù)使用的帽封口。
重力流操作，從BSA中除去NaCl。樣品回收率95%，除鹽99%以上。
PD-10 Desalting Columns能以重力流或離心方式快速脫鹽。藍(lán)色接頭用于離心操作
