GE磁珠系列產(chǎn)品
GE磁珠產(chǎn)品包括Sera-Mag與Mag Sepharose兩類,其中Sera-Mag為1μm超順磁磁珠,可用于mRNA及核酸制備,以及蛋白的小規(guī)模純化與制備;而Mag Sepharose則為高度交聯(lián)的瓊脂糖基架磁珠,一般用于磷酸化肽段富集,免疫沉淀,Pull-down,以及抗體及His標(biāo)簽蛋白的小規(guī)模制備與篩選。
Mag Sepharose ag Sepharose產(chǎn)品具有如下特點(diǎn):
·操作簡單快捷,僅需磁力架即可完成純化;
·方法成熟可靠,結(jié)果重復(fù)性高,提高Pull-down、免疫沉淀實(shí)驗(yàn)的成功率;
·蛋白載量高,高達(dá)22 μg human IgG/ml填料(以Protein G配基為例);
·產(chǎn)品品種齊全,包含TiO2(純化磷酸化肽段)、Protein A、Protein G、Ni螯合(His標(biāo)簽純化)等多種配基。
Sera-Mag Sera-Mag磁珠的主要特點(diǎn)為:
·μm超順磁磁珠,粒徑均一;
·獨(dú)特的菜花狀表面提供了更*的結(jié)合能力;
·磁相應(yīng)時(shí)間短,可快速分離,分離通量及精度高;
·*的膠體穩(wěn)定性及單分散性;
·產(chǎn)品種類齊全,多種配基,如羧基、氨基、Protein A/G融合、親和素、Oligo (dT)供選擇;
·具有常速磁珠(Sera-Mag,單層磁層)及快速磁珠(SpeedBeads,雙層磁層,更快的磁相應(yīng)速度,有效縮短反應(yīng)時(shí)間,提高精確度)可供選擇。
應(yīng)用 | 產(chǎn)品 | 貨號(hào) | 配基/表面 | 包裝 | 備注 |
磷酸化肽段的捕獲和富集 | TiO2?Mag ? Sepharose | 28944010 | TiO2 | 1×500 μl (20% medium slurry) | 提供5×500 μl包裝 |
免疫沉淀、Pull-down、磷酸化蛋白及低豐度蛋白富集(間接法) | Protein A Mag Sepharose | 28944006 | Protein A | 1×500 μl (20% medium slurry) | 提供4×500 μl包裝 |
Protein G Mag Sepharose | 28944008 | Protein G | 1×500 μl (20% medium slurry) | 提供4×500 μl包裝 | |
Protein A/G HP SpinTrap Buffer Kit | 28913567 | 提供目標(biāo)蛋白富集和免疫沉淀配套試劑 | 一套 | ||
Sera-Mag SpeedBeads Protein A/G | 17152104010150 | Protein A/G融合蛋白 | 5 ml (10 mg/ml) | 提供1 ml及100 ml包裝 | |
免疫沉淀、Pull -down、磷酸化蛋白及低豐度蛋白富集(直接法) |
NHS Mag Sepharose | 28944009 | 預(yù)活化NHS集團(tuán) | 1×500 μl (20% medium slurry) | 提供4×500 μl包裝 |
NHS HP SpinTrap Buffer Kit | 28913569 | 提供目標(biāo)蛋白富集和免疫沉淀配套試劑 | 一套 | ||
Sera-Mag magnetic carboxylate | 44152105050250 | 羧基/Hydrophobic | 15 ml (50 mg/ml) | 提供100 ml及1 L包裝 | |
Sera-Mag magnetic carboxylate | 24152105050250 | 羧基/Hydrophilic | 15 ml (50 mg/ml) | 提供100 ml及1 L包裝 | |
Sera-Mag SpeedBeads carboxylate | 65152105050250 | 羧基/Hydrophobic | 15 ml (50 mg/ml) | 提供100 ml及1 L包裝 | |
Sera-Mag SpeedBeads carboxylate | 45152105050250 | 羧基/Hydrophilic | 15 ml (50 mg/ml) | 提供100 ml及1 L包裝 | |
抗體小規(guī)模純化、克隆篩選 | Protein A Mag SepharoseXtra | 28967056 | Protein A | 2×1 ml (10% medium slurry) | 提供5×1 ml包裝 |
Protein G Mag SepharoseXtra | 28967066 | Protein G | 2×1 ml (10% medium slurry) | 提供5×1 ml包裝 | |
AB Buffer Kit | 28903059 | 提供抗體純化配套試劑 | 一套 | ||
純化更大量抗體及抗體片段,請(qǐng)參閱親和層析填料章節(jié) | |||||
His標(biāo)簽蛋白小規(guī)模純化、篩選 | His Mag Sepharose Ni | 28967388 | Ni螯合 | 2×1 ml (5% medium slurry) | 提供5×1 ml及10×1 ml包裝 |
His Mag Sepharose excel | 17371220 | Ni Sepharose excel | 2×1 ml (10% medium slurry) | 提供5×1 ml及10×1 ml包裝 | |
His Buffer Kit | 11003400 | 提供His純化配套試劑 | 一套 | ||
純化更大量His標(biāo)簽片段,請(qǐng)參閱親和層析填料章節(jié) | |||||
生物素標(biāo)簽蛋白、核酸、多肽制備與富集 | Streptavidin Mag Sepharose | 28985738 | 鏈霉親和素 | 2×1 ml (10% medium slurry) | 提供5×1 ml包裝 |
Sera-Mag Streptavidin-Coated Magnetic ? Particles | 30152103010150 | 鏈霉親和素(低生物素結(jié)合量,2500-3500 pmol/mg) | 5 ml (10 mg/ml) | 提供1 ml及100 ml包裝 | |
Sera-Mag Streptavidin-Coated Magnetic ? Particles | 30152104010150 | 鏈霉親和素(中等生物素結(jié)合量,3500-4500 pmol/mg) | 5 ml (10 mg/ml) | 提供1 ml及100 ml包裝 | |
Sera-Mag Streptavidin-Coated Magnetic ? Particles | 30152105010150 | 鏈霉親和素(高生物素結(jié)合量,4500-5500 pmol/mg) | 5 ml (10 mg/ml) | 提供1 ml及100 ml包裝 | |
Sera-Mag ? SpeedBeads Streptavidin -Coated Magnetic Particles |
66152104010150 | 鏈霉親和素(中等生物素結(jié)合量,3500-4500 pmol/mg) | 5 ml (10 mg/ml) | 提供1 ml及100 ml包裝 | |
Sera-Mag ? SpeedBeads Streptavidin -Blocked Magnetic Particles |
21152104010150 | 鏈霉親和素提供 | 5 ml (10 mg/ml) | 提供1 ml及100 ml包裝 | |
Sera-Mag ? SpeedBeads Neutravavidin -Coated Magnetic Particles |
78152104010150 | 中性親和素 | 5 ml (10 mg/ml) | 提供1 ml及100 ml包裝 | |
純化更大量生物素化蛋白或多肽,請(qǐng)參閱親和層析填料章節(jié) | |||||
mRNA提取與制備 | Sera-Mag Oligo(dT)-Coated Magnetic Particles |
38152103010 | Oligo(dT)14 | 5 ml (10 mg/ml) | 提供1 ml及100 ml包裝 |
另外,GE還提供磁力架產(chǎn)品。MagRack 6磁力架,可并行處理6個(gè)1.5 ml離心管的磁珠純化。離心管一旦放在磁力架上,磁珠和目標(biāo)蛋白在幾秒鐘內(nèi)便可集中于磁鐵附近,在輕松去除上清同時(shí)確保蛋白得率。選購貨號(hào)為:28-9489-64。
如果需要處理更大的樣品體積,可以選擇MagRack Maxi磁力架,可處理高達(dá)50 ml樣品。選購貨號(hào)為:28-9864-41。
產(chǎn)品名稱 | 貨號(hào) | 用途 | 包裝 | |
PD-10 重力柱配套 | PD-10 空柱 | 17043501 | 客戶自行灌制親和層析柱 | 50 個(gè) |
LabMate PD-10 Buffer Reservoir | 18321603 | 緩沖液儲(chǔ)槽,可增加PD-10緩沖液體積至25ml | 10 個(gè) | |
PD-10 Spin Adapter | 28923245 | PD-10 在離心機(jī)使用配套 | 10 個(gè) | |
MidiSpin Adapter | 28923244 | MidiTrap在離心機(jī)上使用配套 | 10個(gè) | |
MiniSpin Adapter | 28923243 | MiniTrap在離心機(jī)上使用配套 | 10個(gè) | |
MultiTrap ?96孔板式配套 | Collection Plate | 28403943 | MultiTrap 和PreDictor 配套收集板,96-well plate 500 μl, V-shaped bottom | 5 ×96-well plates |
產(chǎn)品列表:
應(yīng)用 | 產(chǎn)品 | 貨號(hào) | 配基/表面 | 包裝 | 價(jià)格(美金) |
磷酸化肽段的捕獲和富集 | TiO2?Mag?Sepharose | 28-9440-10 | TiO2 | 1×500?μl(20%?medium?slurry) | 138 |
TiO2?Mag?Sepharose | 28-9513-77 | TiO2 | 4×500?μl?(20%?medium?slurry) | 517 | |
免疫沉淀、?Pull-down、磷酸化蛋白及低豐度蛋白富集 (間接法) |
Protein?A?Mag?Sepharose | 28-9440-06 | Protein?A | 1×500?μl(20%?medium?slurry) | 208 |
Protein?A?Mag?Sepharose | 28-9513-78 | Protein?A | 4×500?μl?(20%?medium?slurry) | 801 | |
Protein?G?Mag?Sepharose | 28-9440-08 | Protein?G | 1×500?μl(20%?medium?slurry) | 206 | |
Protein?G?Mag?Sepharose | 28-9513-79 | Protein?G | 4×500?μl?(20%?medium?slurry) | 801 | |
Protein?A/G?HP?SpinTrap?Buffer?Kit | 28-9135-67 | 提供目標(biāo)蛋白富集和免疫沉淀配套試劑 | 一套 | 338 | |
Sera-Mag?SpeedBead?Protein?A/G | 17152104011150 | Protein?A/G?混合模式 | 1ml(10mg/ml) | 184 | |
Sera-Mag?SpeedBead?Protein?A/G | 17152104010150 | Protein?A/G?混合模式 | 5ml(10mg/ml) | 612 | |
Sera-Mag?SpeedBead?Protein?A/G | 17152104010350 | Protein?A/G?混合模式 | 100ml(10mg/ml) | 詢價(jià) | |
免疫沉淀、?Pull-down、磷酸化蛋白及低豐度蛋白富集 (直接法) |
NHS?Mag?Sepharose | 28-9440-09 | 預(yù)活化NHS?基團(tuán) | 1×500?μl(20%?medium?slurry) | 206 |
NHS?Mag?Sepharose | 28-9513-80 | 預(yù)活化NHS?基團(tuán) | 4×500?μl?(20%?medium?slurry) | 801 | |
NHS?HP?SpinTrap?Buffer?Kit | 28-9135-69 | 提供目標(biāo)蛋白富集和免疫沉淀配套試劑 | 一套 | 390 | |
Sera-Mag?magnetic?carboxylate | 44152105050250 | 羧基/Hydrophobic | 15ml(50mg/ml) | 321 | |
Sera-Mag?magnetic?carboxylate | 44152105050350 | 羧基/Hydrophobic | 100ml(50mg/ml) | 詢價(jià) | |
Sera-Mag?magnetic?carboxylate | 44152105050450 | 羧基/Hydrophobic | 1000ml(50mg/ml) | 詢價(jià) | |
Sera-Mag?magnetic?carboxylate | 24152105050250 | 羧基/Hydrophobic | 15ml(50mg/ml) | 321 | |
Sera-Mag?magnetic?carboxylate | 24152105050350 | 羧基/Hydrophobic | 100ml(50mg/ml) | 詢價(jià) | |
Sera-Mag?magnetic?carboxylate | 24152105050450 | 羧基/Hydrophobic | 1000ml(50mg/ml) | 詢價(jià) | |
Sera-Mag?SpeedBead?carboxylate | 65152105050250 | 羧基/Hydrophobic | 15ml(50mg/ml) | 450 | |
Sera-Mag?SpeedBead?carboxylate | 65152105050350 | 羧基/Hydrophobic | 100ml(50mg/ml) | 詢價(jià) | |
Sera-Mag?SpeedBead?carboxylate | 65152105050450 | 羧基/Hydrophobic | 1000ml(50mg/ml) | 詢價(jià) | |
Sera-Mag?SpeedBead?carboxylate | 45152105050250 | 羧基/Hydrophobic | 15ml(50mg/ml) | 450 | |
Sera-Mag?SpeedBead?carboxylate | 45152105050350 | 羧基/Hydrophobic | 100ml(50mg/ml) | 詢價(jià) | |
Sera-Mag?SpeedBead?carboxylate | 45152105050450 | 羧基/Hydrophobic | 1000ml(50mg/ml) | 詢價(jià) | |
SpeedBeads™具有兩層磁層,在磁場中的響應(yīng)速度是Sera-Mag磁珠的2倍,可用于從懸浮液中更快、更完整的分離目標(biāo)分子 |
應(yīng)用 | 產(chǎn)品 | 貨號(hào) | 配基/表面 | 包裝 | 價(jià)格(美金) |
抗體小規(guī)模純化、克隆篩選 | Protein?A?Mag?Sepharose?Xtra | 28-9670-56 | Protein?A | 2×1?ml?(10%?medium?slurry) | 255 |
Protein?A?Mag?Sepharose?Xtra | 28-9670-62 | Protein?A | 5×1?ml?(10%?medium?slurry) | 532 | |
Protein?G?Mag?Sepharose?Xtra | 28-9670-66 | Protein?G | 2×1?ml?(10%?medium?slurry) | 255 | |
Protein?G?Mag?Sepharose?Xtra | 28-9670-70 | Protein?G | 5×1?ml?(10%?medium?slurry) | 532 | |
Ab?Buffer?Kit | 28-9030-59 | 提供抗體純化配套試劑 | 一套 | 158 | |
His?標(biāo)簽蛋白小規(guī)模純化、篩選 | His?Mag?Sepharose?Ni | 28-9673-88 | Ni螯合 | 2×1?ml?(5%?medium?slurry) | 146 |
His?Mag?Sepharose?Ni | 28-9673-90 | Ni螯合 | 5×1?ml?(5%?medium?slurry) | 324 | |
His?Mag?Sepharose?Ni | 28-9799-17 | Ni螯合 | 10×1?ml?(5%?medium?slurry) | 590 | |
His?Mag?Sepharose?excel | 17-3712-20 | Ni?Sepharose?excel | 2×1?ml?(10%?medium?slurry) | 222 | |
His?Mag?Sepharose?excel | 17-3712-21 | Ni?Sepharose?excel | 5×1?ml?(10%?medium?slurry) | 456 | |
His?Mag?Sepharose?excel | 17-3712-22 | Ni?Sepharose?excel | 10×1?ml?(10%?medium?slurry) | 734 | |
His?Buffer?Kit | 11-0034-00 | 提供His純化配套試劑 | 一套 | 155 | |
生物素標(biāo)簽蛋白、核酸、多 肽制備與富集 |
Streptavidin?Mag?Sepharose | 28-9857-38 | 鏈霉親和素 | 2×1?ml?(10%?medium?slurry) | 255 |
Streptavidin?Mag?Sepharose | 28-9857-99 | 鏈霉親和素 | 5×1?ml?(10%?medium?slurry) | 537 | |
Sera-Mag?Streptavidin-Coated?Magnetic | 30152103011150 | 鏈霉親和素?(low?binding?capacity) | 1ml(10mg/ml) | 167 | |
Sera-Mag?Streptavidin-Coated?Magnetic | 30152103010150 | 鏈霉親和素?(low?binding?capacity) | 5ml(10mg/ml) | 668 | |
Sera-Mag?Streptavidin-Coated?Magnetic | 30152104011150 | 鏈霉親和素?(medium?binding?capacity) | 1ml(10mg/ml) | 193 | |
Sera-Mag?Streptavidin-Coated?Magnetic | 30152104010150 | 鏈霉親和素?(medium?binding?capacity) | 5ml(10mg/ml) | 771 | |
Sera-Mag?Streptavidin-Coated?Magnetic | 30152105011150 | 鏈霉親和素?(high?binding?capacity) | 1ml(10mg/ml) | 218 | |
Sera-Mag?Streptavidin-Coated?Magnetic | 30152105010150 | 鏈霉親和素?(high?binding?capacity) | 5ml(10mg/ml) | 900 | |
Sera-Mag?SpeedBeads?Streptavidin-Coated?Magnetic | 66152104011150 | 鏈霉親和素?(medium?binding?capacity) | 1ml(10mg/ml) | 321 | |
Sera-Mag?SpeedBeads?Streptavidin-Coated?Magnetic | 66152104010150 | 鏈霉親和素?(medium?binding?capacity) | 5ml(10mg/ml) | 1028 | |
Sera-Mag?SpeedBeads?Streptavidin-Blocked?Magnetic | 21152104011150 | 鏈霉親和素?(low?nonspecific?binding) | 1ml(10mg/ml) | 450 | |
Sera-Mag?SpeedBeads?Streptavidin-Blocked?Magnetic | 21152104010150 | 鏈霉親和素?(low?nonspecific?binding) | 5ml(10mg/ml) | 1028 | |
Sera-Mag?SpeedBeads?Streptavidin-Blocked?Magnetic | 21152104010350 | 鏈霉親和素?(low?nonspecific?binding) | 100ml(10mg/ml) | 詢價(jià) | |
Sera-Mag?SpeedBeads?Neutravavidin-Coated?Magnetic | 78152104011150 | 中性親和素 | 1ml(10mg/ml) | 321 | |
Sera-Mag?SpeedBeads?Neutravavidin-Coated?Magnetic | 78152104010150 | 中性親和素 | 5ml(10mg/ml) | 1028 | |
mRNA提取與制備 | Sera-Mag?Oligo(dT)-Coated?Magnetic | 38152103011150 | Oligo(dT)14 | 1ml(10mg/ml) | 161 |
Sera-Mag?Oligo(dT)-Coated?Magnetic | 38152103010150 | Oligo(dT)14 | 5ml(10mg/ml) | 643 | |
Sera-Mag?Oligo(dT)-Coated?Magnetic | 38152103010350 | Oligo(dT)14 | 100ml(10mg/ml) | 詢價(jià) |