親和配體包覆聚苯乙烯 Affinity Ligand Coated Polystyrene
Affinity Ligand Coated Polystyrene?親和配體包覆微球
When circumstances call for the rapid development of microsphere reagents, particles coated with affinity binding proteins offer a ready solution. Affinity binding protocols are straightforward, typically involving a simple incubation of the spheres with the appropriate ligand. Binding proteins also provide an opportunity to optimally orient the ligand, as with the end-point immobilization of biotinylated oligonucleotides to streptavidin-coated microspheres, or the Fc-specific immobilization of antibodies via Protein A or G.
Particles coated with binding proteins also find significant use in purifications. Streptavidin-coated microspheres are often used in the isolation of biotinylated PCR amplicons. Protein A and Protein G microspheres are utilized for purification of antibodies and Fc-tagged fusion proteins, as well as for the recovery of Ab/Ag complexes in immunoprecipitation.
當環(huán)境要求迅速發(fā)展的微球試劑，粒子包覆親和結(jié)合蛋白提供了一個現(xiàn)成的解決方案。親和結(jié)合協(xié)議是直截了當?shù)?，通常涉及球與適當配體的簡單孵育。結(jié)合蛋白還提供了一個佳的定位配體的機會，例如生物素化寡核苷酸在鏈霉親和素包被的微球上的末端固定，或通過蛋白A或蛋白G對抗體的fc特異性固定。
包被結(jié)合蛋白的顆粒在純化中也有重要的用途。鏈霉親和素包被微球常用于生物素化PCR擴增產(chǎn)物的分離.蛋白A和蛋白G微球用于純化抗體和Fc標記融合蛋白，以及免疫沉淀中抗體/Ag復(fù)合物的回收。
STREPTAVIDIN? 鏈霉親和素
Our streptavidin and SuperAvidin™ coated microspheres offer simple and extremely stable binding of biotinylated molecules, such as antibodies, peptides, and oligonucleotides for use in diagnostics, screening, and affinity purifications. See?TechNote 101, ProActive??Microspheres. Streptavidin coated microspheres are available as ~1% solids (w/v) aqueous suspensions in four standard volumes: 1mL, 2mL, 5mL, or 10mL.
我們的鏈親和素和超親和素™包被微球提供簡單和非常穩(wěn)定的結(jié)合生物素化分子，如抗體，肽和寡核苷酸，用于診斷，篩選和親和純化?？匆奣echnote 101，未雨綢繆?微球。鏈霉親和素包被微球可作為1%固體(w/v)的水懸浮液，分4種標準體積：1ml、2mL、5ml或10 ml。
STREPTAVIDIN POLYSTYRENE? 鏈霉親和素聚苯乙烯
| Catalog Number | Nominal Diameter | Specification Range | Product Data Sheet |
|---|---|---|---|
| CP01000 | 0.10μm | 0.090 – 0.110μm | PDS 721.pdf |
| CP01001 | 0.20μm | 0.190 – 0.210μm | PDS 721.pdf |
| CP01003 | 0.50μm | 0.470 – 0.530μm | PDS 721.pdf |
| CP01004 | 1.00μm | 0.95 – 1.05μm | PDS 721.pdf |
| CP01005 | 3.0μm | 2.80 – 3.20μm | PDS 721.pdf |
| CP01006 | 5.00μm | 4.80 – 5.20μm | PDS 721.pdf |
| CP01007 | 10.00μm | 9.50 – 10.50μm | PDS 721.pdf |
| CP01008 | 15.0μm | 14.50 – 15.50μm | PDS 721.pdf |
REFERENCES
Kahanovitz L, Seker E, Marks RS, Yarmush ML, Konry T, & Russell SJ. (2016)?Development of a microsphere-based system to facilitate real-time insulin monitoring.?Journal of diabetes science and technology, 10(3), 689-696.(10μm SA microspheres coated with biotin-anti-insulin Ab for insulin monitoring in a microdevice)
Streptavidin?magnetic?polymer?
The surfaces of Encapsulated magnetic microspheres are expected to be free of iron oxide, while the Classical version will have some exposed iron oxide. COMPEL microspheres (UMC catalog codes) are highly uniform and spherical. Fluorescent magnetic microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 5mL, 10mL, and 100mL. See spectra for available?fluorescent dyes.
鏈霉親和素磁性聚合物
包覆磁性微球的表面不含氧化鐵，而經(jīng)典的磁性微球表面會有一些暴露的氧化鐵。強制微球(UMC編錄碼)具有高度的均勻性和球形。熒光磁性微球可作為1%固體(w/v)的水懸浮液在這些標準體積：1ml，5ml，10毫升和100毫升。見可用光譜熒光染料.
| Catalog Number | Description | Nominal Diameter | Specification Range | Product Data Sheet |
|---|---|---|---|---|
| CME0101 | SA / Encapsulated Magnetic Polymer | 0.86μm | 0.7 – 1.2μm | PDS 721.pdf |
| UMC0100 | SA/ COMPEL | 3μm | 2.50 – 3.50μm | PDS 721.pdf |
| UMC0101 | SA / COMPEL | 6μm | 5.50 – 6.50μm | PDS 721.pdf |
| UMC0102 | SA / COMPEL | 8μm | 7.50 – 8.50μm | PDS 721.pdf |
STREPTAVIDIN FLUORESCENT POLYSTYRENE
| Catalog Number | Fluorescence Color | Nominal Diameter | Specification Range | Product Data Sheet |
|---|---|---|---|---|
| CFDG001 | SA / Dragon Green | 0.20μm | 0.190 – 0.210μm | PDS 721.pdf |
| CFDG002 | SA / Dragon Green | 0.40μm | 0.370 – 0.430μm | PDS 721.pdf |
| CFFR002 | SA / Flash Red | 0.40μm | 0.370 – 0.430μm | PDS 721.pdf |
| CFDG003 | SA / Dragon Green | 0.50μm | 0.470 – 0.530μm | PDS 721.pdf |
| CFDG004 | SA / Dragon Green | 1.00μm | 0.95 – 1.05μm | PDS 721.pdf |
| CFFR004 | SA / Flash Red | 1.00μm | 0.95 – 1.05μm | PDS 721.pdf |
STREPTAVIDIN DYED POLYSTYRENE
Streptavidin-coated dyed microspheres supplied as ~1% solids (w/v) aqueous suspensions.
鏈霉親和素染色聚苯乙烯
鏈霉親和素涂層染色微球提供~1%固體(w/v)的水懸浮液。
| Catalog Number | Coating / Dye | Nominal Diameter | Specification Range |
|---|---|---|---|
| CDCR001 | SA / Crimson Red | 0.20μm | 0.190 – 0.210μm |
| CDCB001 | SA / Cabo Blue | 0.20μm | 0.190 – 0.210μm |
REFERENCES
Sepp, A, Tawfik, D, Griffiths, D(2002)?Microbead display by in vitro compartmentalisation: selection for binding using flow cytometry. FEBS Letters V532(3)P455–458
Kahanovitz L, Seker E, Marks RS, Yarmush ML, Konry T, Russell SJ (2016)?Development of a Microsphere-Based System to Facilitate Real-Time Insulin Monitoring.?J Diabetes Sci Technol 10(3):689-96
Tanga MYH, Shum HC (2016)?One-step immunoassay of C-reactive protein using droplet microfluidics?Lab Chip, 16, 4359-4365
BIOTIN
Our biotin-coated microspheres have been fully characterized in terms of their ability to bind free avidin, and therefore will require minimal optimization when determining the correct concentration of ligand to be bound. Biotin coated beads are supplied as ~1% solids (w/v) aqueous suspensions. See?TechNote 101, ProActive??Microspheres, for sample applications and procedures.
生物素
我們的生物素包覆的微球在結(jié)合游離親和素的能力方面已經(jīng)完全被表征，因此在確定正確的配體濃度時，需要進行最小的優(yōu)化。生物素包覆微珠是以~1%固體(w/v)的水懸浮形式提供的?？匆奣echnote 101，未雨綢繆?用于樣品應(yīng)用和程序的微球。
| Catalog Number | Description | Nominal Diameter | Specification Range | Product Data Sheet |
|---|---|---|---|---|
| CP10000 | Polymeric / Biotin | 10μm | 9.50 – 10.50μm | PDS 724.pdf |
PROTEIN A AND PROTEIN G
Protein A and Protein G are proteins that readily bind the Fc regions of many types of antibodies. For this reason, they are used extensively for affinity purification of antibodies from serum or cell culture supernatant, and for capture of immunoprecipitated protein complexes. When Protein A and Protein G microspheres are used as reagents in tests and assays, the immobilized antibodies may be crosslinked to the Protein A- or Protein G-coated support for long-term stability. See?TechNote 101, ProActive??Microspheres. Protein A and Protein G coated microspheres are available as ~1% solids (w/v) aqueous suspensions in four standard volumes: 1mL, 2mL, 5mL, or 10mL.
蛋白A和蛋白G
蛋白A和蛋白G是很容易與多種抗體的FC區(qū)結(jié)合的蛋白質(zhì)。因此，它們被廣泛用于血清或細胞培養(yǎng)上清液中抗體的親和純化，以及免疫沉淀蛋白復(fù)合物的捕獲。當?shù)鞍譇和蛋白G微球作為試劑進行試驗和檢測時，固定化抗體可以交聯(lián)到蛋白A或蛋白G包被載體上，以保持長期穩(wěn)定性?？匆奣echnote 101，未雨綢繆?微球。蛋白A和蛋白G包覆的微球可作為1%固體(w/v)的水懸浮液，分4種標準體積：1ml、2mL、5ml或10 ml。
| Catalog Number | Description | Nominal Diameter | Specification Range | Product Data Sheet |
|---|---|---|---|---|
| CP02000 | Polymer / Protein A | 1.00μm | 0.95-1.05μm | PDS 722.pdf |
| CP02001 | Polymer / Protein A | 5.00μm | 4.80-5.20μm | PDS 722.pdf |
| CP02002 | Polymer / Protein A | 10.0μm | 9.50-10.50μm | PDS 722.pdf |
| PMG3N | ProMag™ (Magnetic) / Protein G | 3μm | 2.70 – 3.30μm | PDS 722.pdf |