Bio-serv F1258 液體酒精飼料 Lieber-DeCarli ’82, Ethanol說(shuō)明書

貨號(hào)：F1258

英文名稱：Rodent Liquid Diet, Lieber-DeCarli ’82, Ethanol

規(guī)格：4 Liters/Bag

Directions for Preparing 4 Liters of Diet
These directions are for preparing diets that contain 36% kcal from ethanol. To prepare diets with modified
ethanol derived calories, see conversion chart.
1. Add dry mix, Product# F1258 (523.6 grams/4 Liters) to a blender container.
2. Measure 3400 mL of cold water (4 degrees Celsius) to the container.
3. Add 268 mL of 95% ethanol (not provided) to the container.
5. Blend for 20-30 seconds at low speed.
6. Product ready to dispense into the feeding tubes

準(zhǔn)備 4 升飲食的說(shuō)明
這些說(shuō)明用于制備含有 36% 千卡乙醇的飲食。 準(zhǔn)備含有改良的飲食
乙醇衍生的卡路里，見換算表。
1. 將干燥混合物 Product# F1258（523.6 克/4 升）添加到攪拌器容器中。
2. 稱量 3400 毫升冷水（4 攝氏度）到容器中。
3. 向容器中加入 268 mL 95% 乙醇（未提供）。
5. 低速攪拌 20-30 秒。
6. 準(zhǔn)備分配到進(jìn)料管中的產(chǎn)品

Feeding Instructions
It is recommended to feed the liquid diet in liquid diet feeding tubes as standard water sipper bottles can
become clogged with particulates. To fill the liquid diet feeding tubes, place thumb over the feed well and fill
with liquid diet from the top. Maintain thumb on the feed well while screwing cap on securely and invert tube
to release any trapped air bubbles. Place in feeding tube holder in cage.
The prepared diet should be maintained under refrigeration and should be dispensed within 3 days.
The prepared diet should not be kept longer than 48 hours at room temperature to prevent diet deterioration.
If diet has been prepared the previous day, invert bottle several times prior to dispensing to re-suspend
particles, which may have settled.
The average mouse will consume 20-30 mL of liquid diet per day. The average rat will consume 80-100 mL
of liquid diet per day. Intake will vary with the size and sex of the animal.
A gradual introduction of the liquid diet is recommended. Introduce the liquid diet in the presence of the
standard solid diet. Over a period of 3-5 days, increase the amount of liquid diet while decreasing the amount
of solid diet to allow the animal to acclimate. Additional acclimation time is required for ethanol containing
diets. It is recommended that the animals be gradually introduced to the ethanol diet as follows:
Day 1-3 Feed mixture of 1/3 ethanol diet and 2/3 control diet
Day 4-7 Feed mixture 2/3 of ethanol diet with 1/3 of control diet
Day 8 Feed ethanol diet only
The liquid diet is both a food and water source. A separate water sipper bottle may be provided, but may not be consumed.

喂養(yǎng)說(shuō)明
建議在流食飼管中喂食流食，因?yàn)闃?biāo)準(zhǔn)的吸水瓶可以、被顆粒物堵塞。要填充液體飼料飼管，請(qǐng)將拇指放在飼料槽上并填充從頂部流質(zhì)飲食。將拇指放在進(jìn)料口上，同時(shí)擰緊蓋子并倒置管釋放任何被困的氣泡。放置在籠子里的飼管架上。
準(zhǔn)備好的飲食應(yīng)冷藏保存，并應(yīng)在 3 天內(nèi)分發(fā)。
準(zhǔn)備好的日糧在室溫下保存時(shí)間不應(yīng)超過(guò) 48 小時(shí)，以防止日糧變質(zhì)。
如果前一天已經(jīng)準(zhǔn)備好飲食，在分配前將瓶子倒置幾??次以重新懸浮可能已經(jīng)沉降的顆粒。
普通小老鼠每天會(huì)消耗 20-30 毫升的流質(zhì)飲食。平均大老鼠會(huì)消耗 80-100 毫升每天流質(zhì)飲食。攝入量會(huì)因動(dòng)物的大小和性別而異。
建議逐漸引入流質(zhì)飲食。在存在以下情況時(shí)引入流質(zhì)飲食
標(biāo)準(zhǔn)的固體飲食。在3-5天的時(shí)間內(nèi)，增加流質(zhì)飲食的量，同時(shí)減少量固體飲食，讓動(dòng)物適應(yīng)。含乙醇需要額外的馴化時(shí)間飲食。建議將動(dòng)物逐漸引入乙醇飲食，

如下所示：
第 1-3 天 1/3 乙醇日糧和 2/3 對(duì)照日糧的混合飼料
第 4-7 天 飼料混合物 2/3 乙醇日糧和 1/3 對(duì)照日糧
第 8 天 僅飼喂乙醇飲食
流質(zhì)飲食既是食物又是水源?？赡軙?huì)提供一個(gè)單獨(dú)的吸水瓶，但可能不會(huì)被消耗。