Amersham 帶正電荷尼龍膜 Hybond-N+ (30 cm * 3 m)

Amersham Hybond-N+ 帶正電荷尼龍膜 0.45um 30cmx3m RPN303B
帶正電荷的尼龍膜與核酸高達(dá)600μg/cm2的結(jié)合能力。建議使用放射性和非放射性的化學(xué)發(fā)光法和chemifluorescence檢測系統(tǒng)。
Amersham公司Hybond™- N +產(chǎn)品在傳統(tǒng)的Southern雜交有著出色的靈敏度。核酸樣品可能是固定的，通過紫外線照射或烘烤，雖然紫外線固定是最好的最大的重復(fù)性。Hybond?N +是不適合堿印跡和固定。

Amersham Hybond-N是一種堅(jiān)固的支撐膜，具有固有的親水性，無需預(yù)潤濕。 紫外光照時(shí)可以快速、輕松地將核酸與尼龍膜交聯(lián)起來。 使用緩沖液體積較少時(shí)，建議使用Hybond-NX。

Amersham Hybond™-N+

• Positively charged nylon membrane with a binding capacity for nucleic acids up to 600 μg/cm²?recommended for use with radioactive or nonradioactive chemiluminescence and chemifluorescence detection systems.
• Recommended for all high density membrane arrays.

Technical Information

Amersham Hybond™-N+ yields excellent sensitivity in conventional Southern blotting. Nucleic acid samples may be fixed by UV exposure or baking, although UV fixation is best for maximum reproducibility. Hybond-N+ is not suitable for Alkali blotting and fixation.

• Multiple reprobing is possible without loss of membrane integrity.
• QC tested in genomic Southern blots using GE Healthcare products.
• Special sizes available on request.

Human genomic DNA (1, 0.5, 0.125, 0.06 and 0.03 μg loadings) digested with HindIII and transferred to Hybond-N+. Hybridizaton with?A. N-ras, B. p53, and C.?bcl-2 labeled DNA probes and detected using Gene Images™ random-prime labeling with CDP-Star™ detection.

Membranes should be stored at room temperature in a clean dry atmosphere away from excessive heat, light, and noxious fumes. To prevent contamination, handle membranes using gloves or blunt-ended forceps. Hybond-N+ is stable for up to 3 years. Membranes should be kept in the bags in which they are received. Performance is consistent when stored under the recommended conditions.