Hampton HR2-707 3.4 M Sodium malonate pH 4.0
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Hampton 丙二酸鈉 3.4 M Sodium malonate pH 4.0
Sodium malonate??丙二酸鈉
APPLICATIONS??應用
- Crystallization grade Sodium malonate for formulating screens or for optimization用于篩選或優(yōu)化的結晶級丙二酸鈉
FEATURES??特征
- Sterile filtered solution
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/μL)
- 無菌過濾液
- 在1+超純水中配制：在25°C下電阻率為18.2兆，總有機碳<5 ppb，無細菌(<1細菌(CFU/ml))，無熱原(<0.03內毒素(EU/ml))，無RNase(<0.01 ng/mL)和無DNase(<4 pg/L)
DESCRIPTION
The Sodium malonate offered by Hampton Research is Malonic acid (CAS Number 141-82-2) titrated to pH 4.0-8.0 using Sodium hydroxide as described in the publication “A comparison of salts for the crystallization of macromolecules. Alexander McPherson. Protein Science (2001), 10:418-422.”. In this same publication, Sodium malonate was reported to be more successful than any other salt, resulting in the crystallization of 19 of the 23 macromolecules, almost twice as effective as the next most successful salt, which was a draw between sodium acetate, sodium tartrate, sodium formate, and ammonium sulfate. The high success rate of Sodium malonate in producing crystals was even more impressive when an overall unique success rate with individual macromolecules was considered.
The use of Sodium malonate in place of Ammonium sulfate has been reported in one instance to improve the reproducibility of crystals and improve resistance to physicochemical shocks. Sodium malonate also acted as a good cryoprotectant (Xing and Xu 2003).
One may combine the four different sodium malonate stocks to create a pH versus sodium malonate concentration grid for screening or optimization. Refer to the pH/Dilution Table for Sodium malonate.
Based on the structure of malonic acid titrated with sodium hydroxide, there are no chiral carbons so it is not characterized as having enantiomeric structures, such as D or L.
Synonyms: Propanedioic acid or Malonic acid
C3H4O4?(before titration with NaOH)
Mr?104.06 (before titration with NaOH)
CAS Number 141-82-2
EC Number 205-503-0
Beilstein Registry Number 1751370
Merck 14,5710
RTECS OO0175000
MDL Number MFCD00002707
Purity > 99.0%
pKa1 2.8
pKa2 5.7
描述
Hampton研究提供的丙二酸鈉是蘋果酸(化學文摘社編號141-82-2)，使用氫氧化鈉滴定至pH4.0-8.0，載于出版物“大分子結晶鹽的比較”，Alexander McPherson，“蛋白質科學”(2001)，10：418-422。在同一份出版物中，據報道，丙二酸鈉比任何其他鹽都更成功，導致23種大分子中的19種結晶，幾乎是第二種最成功的鹽的兩倍，即乙酸鈉、酒石酸鈉、甲酸鈉和硫酸銨之間的結晶。當考慮到單個大分子的整體獨特成功率時，丙二酸鈉在晶體生產中的高成功率更令人印象深刻。
有報道稱，用丙二酸鈉代替硫酸銨可提高晶體的重現性，提高其抗物理化學沖擊的能力。丙二酸鈉也是一種良好的冷凍保護劑(邢和旭，2003年)。
一種方法是將四種不同的丙二酸鈉組合起來，形成一個pH值相對于丙二酸鈉濃度的網格，用于篩選或優(yōu)化。請參閱丙二酸鈉的pH/稀釋表。
根據氫氧化鈉滴定丙二酸的結構，不存在手性碳，不具有對映體結構，如D或L。
同義詞：丙二酸或丙二酸
C3H4O4(用氫氧化鈉滴定前)
Mr104.06(用氫氧化鈉滴定前)
核證機關編號141-82-2
歐共體號碼205-503-0
伯爾斯坦登記處編號1751370
默克14,5710
RTECS OO 0175000
MDL號碼MFCD 00002707
純度>99.0%
PKA 1 2.8
PKa25.7
HR2-747 Measured Conductivity Range: 73.3 – 75.3 mS/cm at 25°C
HR2-747 Measured Refractive Index Range: 1.38365 – 1.38425 at 20°C
HR2-749 Measured Conductivity Range: 77.2 – 78.0 mS/cm at 25°C
HR2-749 Measured Refractive Index Range: 1.39264 – 1.39300 at 20°C
HR2-751 Measured Conductivity Range: 73.4 – 75.2 mS/cm at 25°C
HR2-751 Measured Refractive Index Range: 1.39938 – 1.39973 at 20°C
HR2-707 Measured Conductivity Range: 71.0 – 73.7 mS/cm at 25°C
HR2-707 Measured Refractive Index Range: 1.40090 – 1.40098 at 20°C
HR2-807 Measured Conductivity Range: 71.8 – 75.0 mS/cm at 25°C
HR2-807 Measured Refractive Index Range: 1.40106 – 1.40115 at 20°C
HR2-747測量電導率范圍：25°C下73.3~75.3 ms/cm
HR2-747測量折射率范圍：1.38365~1.38425℃，20°C
HR2-749在25°C下的電導率范圍：77.2~78.0 ms/cm
HR2-749測量折射率范圍：1.39264~1.39300℃，20°C
HR2-751測量電導率范圍：25°C下73.4~75.2 ms/cm
HR2-751測量折射率范圍：1.39938~1.39973℃，20°C
HR2-707測量電導率范圍：25°C下71.0~73.7 ms/cm
HR2-707測量折射率范圍：1.40090~1.40098℃，20°C
HR2-807測量電導率范圍：25°C下71.8~75.0 ms/cm
HR2-807測量折射率范圍：1.40106~1.40115℃，20°C
| HR2-747 | 3.4 M Sodium malonate pH 4.0 | 200 mL |
| HR2-749 | 3.4 M Sodium malonate pH 5.0 | 200 mL |
| HR2-751 | 3.4 M Sodium malonate pH 6.0 | 200 mL |
| HR2-707 | 3.4 M Sodium malonate pH 7.0 | 200 mL |
| HR2-807 | 3.4 M Sodium malonate pH 8.0 | 200 mL |
REFERENCES? 參考文獻
1. A comparison of salts for the crystallization of macromolecules. Alexander McPherson. Protein Science (2001), 10:418-422.
2. Crystallization of the PX domain of cytokine-independent survival kinase (CISK): improvement of crystal quality for X-ray diffraction with sodium malonate. Yi Xing and Wenqing Xu. Acta Cryst. (2003). D59, 1816-1818.
3. Malonate: a versatile cryoprotectant and stabilizing solution for salt-grown macromolecular crystals. T. Holyoak, T. D. Fenn, M. A. Wilson, A. G. Moulin, D. Ringe and G. A. Petsko. Acta Cryst. (2003). D59, 2356-2358.
4. Purification, crystallization and preliminary crystallographic analysis of Est25: a ketoprofen-specific hormone-sensitive lipase. Kim et al Acta Cryst. (2007). F63, 579–581.