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Ricinus Communis Agglutinin I (RCA I, RCA120), Agarose bound
Description
Agarose bound?Ricinus communis agglutinin I is prepared using our affinity-purified lectins.?This lectin consists of two subunits of 60 kDa which can be dissociated by reducing agents into closely related chains between 27 kDa and 33 kDa. One of the chains appears to be common to the B chain of another castor bean lectin, ricin, while the other chain is unique to RCA I.
Features:
- Bead diameter ranges in size from 45-165 microns
- Matrix is stable in solutions at pH 3-11 as well as many organic solvents
- Immobilized lectins are prepared using affinity purified lectins
- Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions
- Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
- No residual charges present after conjugation. ?This minimizes non-specific binding to the matrix
- Product supplied as a 1:1 suspension in buffer
- Inhibiting/Eluting Sugar: 200 mM galactose or lactose?or?Glycoprotein Eluting Solution (ES-2100)
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Specifications
| Unit Size | 2 ml, 10 ml |
|---|---|
| Applications | Glycobiology, Affinity Chromatography |
| Recommended Storage | 2-8 °C DO NOT FREEZE |
| Solution | 10 mM HEPES, pH 7.5, 0.15 M NaCl, 20 mM lactose, 0.08% sodium azide |
| Recommended Usage | Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-2100. Alternatively, 200 mM galactose or lactose can be used. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage. |
| Matrix Conjugate | Lectins |
| Sugar Specificity | Galactose, Lactose |
| Conjugate | Agarose |
Documents
- Safety Data Sheet
- Lectins in Histochemistry, ELISA, and Western Blot Applications
- Download CoA
- Datasheet
Product FAQs
I purchased an agarose bound lectin from Vector Labs. Do you have a protocol outline on how this may be applied in a column format?
What are recommended conditions for using the agarose-lectin in chromatography?
Related Products
Ricinus Communis Agglutinin I (RCA I, RCA120), Biotinylated Catalog #: B-1085-5
Glycoprotein Eluting Solution for Galactose/GalNAc Binding Lectins Catalog #: ES-2100-100
Ricinus Communis Agglutinin I (RCA I, RCA120), Fluorescein Catalog #: FL-1081-5
Ricinus Communis Agglutinin I (RCA I, RCA120), Unconjugated Catalog #: L-1080-10
Ricinus Communis Agglutinin I (RCA I, RCA120), Rhodamine Catalog #: RL-1082-5
Galactose Catalog #: S-9003
Lactose Catalog #: S-9004
Technical Information
Agarose bound* Ricinus communis agglutinin I is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.
This coupling method provides several advantages over the traditional cyanogen bromide procedure:
- Maximum carbohydrate binding activity of the coupled lectins is retained
- Linkage is stable over a range of pH values
Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.
*4 mg lectin/ml gel