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Vector Labs 公司是世界著名的生物學檢測試劑公司，七十年代其在全球首推的生物素-親和素系統(tǒng)引領(lǐng)了生物學檢測方法的重大革命，該系統(tǒng)被普遍視為目前最靈敏、最可靠與最有效的染色系統(tǒng)，并被廣泛應(yīng)用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統(tǒng)仍在不斷地發(fā)展，以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產(chǎn)品的主要供應(yīng)商，并提供大量的新產(chǎn)品，如酶底物、神經(jīng)元示蹤劑及蛋白質(zhì)、糖類與核酸的標記、分離與檢測試劑。
NEUROBIOTIN?-PLUS Tracer
Description
NEUROBIOTIN-Plus Tracer is a biotin label that is impervious to cleavage and breakdown by biotinidase. It is used for visualizing neural architecture and for the identification of gap junction coupling
Features:
- Fixable primary amine
- Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
- Can be delivered by many routes such as intracellular electrodes, microinjection, cut-loading, or scrape-loading
- Biotin label can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods
Advantages of NEUROBIOTIN?Tracer over biocytin and other neuronal labels:
- Better solubility
- More efficiently iontophoresed
- Remains in cell longer
- Non-toxic
- Can be fixed with formalin or glutaraldehyde
Specifications
| Unit Size | 5 mg |
|---|---|
| Recommended Storage | 2-8 °C (desiccated). Once in solution, store frozen. This product does not contain an antimicrobial agent. |
| Recommended Usage | Fixable with formaldehyde or glutaraldehyde |
| Solution | 10% solutions can be made in water, buffers, 1 M KCl, 1 M NaCl, or dimethylsulfoxide. |
| Molecular Weight | 358 |
| Neuronal Tracer – Direction of Transport | Anterograde/Retrograde |
| Detection Method | Avidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence |
Documents
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
NEUROBIOTIN? 488 Tracer Catalog #: SP-1125-2
NEUROBIOTIN? 350 Tracer Catalog #: SP-1155-2
NEUROBIOTIN? Tracer Catalog #: SP-1120
Citations
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Investigators have identified rapid degradation of injected tracers such as biocytin due to the presence of biotinidase activity in brain tissue*. This loss of stability of the tracer significantly reduces the viable postinjection period and may compromise complete detection of a neural network. NEUROBIOTIN-Plus was designed to be impervious to cleavage and breakdown by biotinidase. This newly synthesized biotin-containing compound contains fixable amine groups, is highly soluble and has a low molecular weight. These characteristics make it an ideal candidate for experiments requiring long postinjection survival times and optimal uptake along the entire neural tract.
NEUROBIOTIN-Plus shares the same key advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels:
- Better solubility
- More efficiently iontophoresed
- Remains in cell longer
- Non-toxic
- Can be fixed with formalin or glutaraldehyde/li>
NEUROBIOTIN-Plus can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods.
*Mishra, A. et al (2010) ACS Chem. Neurosci. 1:129-138.