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Horse Anti-Mouse IgG Antibody (H+L), Biotinylated, R.T.U.
Description
R.T.U. Biotinylated Horse Anti-Mouse IgG Antibody?is?used in an avidin-biotin or streptavidin-biotin detection system.?These antibodies can be used for tissue and cell staining, ELISAs, and blots.
Features:
- Affinity-purified, ultrapure, high affinity antibody?
- Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species. (For rat tissue applications, please see anti-mouse, rat adsorbed, secondary antibodies.)?
- Recognizes both heavy and light chains (H+L)?
- Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody?
- Can be used for tissue and cell staining, ELISAs, and blots?
- Included in the?VECTASTAIN? ABC kits
- Supplied in a convenient ready-to-use prediluted, stabilized solution?
Specifications
| Unit Size | 50 ml |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs |
| Concentration | Ready-to-use solution of biotinylated horse anti-mouse IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative. |
| Recommended Storage | 2-8 °C |
| Recommended Usage | Following incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Mouse IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN? ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl. |
| Target Species | Mouse |
| Conjugate | Biotinylated |
| Host Species | Horse |
| Format | Ready-to-Use |
Documents
- Safety Data Sheet
- Download CoA
- Datasheet
Product FAQs
I am performing IHC on tissue sections using a mouse IgG primary antibody. My detection system is a biotinylated anti-mouse IgG secondary antibody, a VECTASTAIN? Elite ABC peroxidase kit (PK-6100), in combination with a DAB substrate kit (SK-4100), and I do not see any staining. How do I know if the end detection reagents are working?
Make a fresh working solution of DAB substrate per instructions. Place a small volume (~1 mL) of this DAB substrate into a clean glass test tube. To this 1 ml aliquot, add one drop (~50 μL) of Reagent B only from the VECTASTAIN? Elite ABC kit. If the HRP enzyme is active and the DAB reacts with it, an immediate color change will be observed. This indicates the end detection reagents are working appropriately.
Related Products
VECTASTAIN? Elite ABC-HRP Reagent, Peroxidase, R.T.U. Catalog #: PK-7100
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Texas Red? Avidin DCS, Cell Sorting Grade Catalog #: A-2016-1
VECTASTAIN? ABC-AP Kit, Alkaline Phosphatase (Standard) Catalog #: AK-5000
VECTASTAIN? ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-4000
VECTASTAIN? Elite ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-6100
Normal Horse Serum Blocking Solution Catalog #: S-2000-20
Streptavidin, DyLight? 488 Catalog #: SA-5488-1
Streptavidin, DyLight? 549 Catalog #: SA-5549-1
Streptavidin, DyLight? 594 Catalog #: SA-5594-1
Streptavidin, DyLight? 649 Catalog #: SA-5649-1
Streptavidin, Peroxidase, R.T.U. Catalog #: SA-5704-100
Citations
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