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Horse Anti-Mouse IgG Antibody, rat adsorbed (H+L), Biotinylated
Description
The Biotinylated Horse Anti-Mouse IgG, rat adsorbed,?is designed for use with rat tissues that may contain endogenous rat immunoglobulins. This adsorbed antibody allows primary antibodies made in mouse to be used on rat tissues.
Features:
- Recognizes both heavy and light chains (H+L)?
- Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody?
- Can be used for tissue and cell staining, ELISAs, and blots?
- Supplied in solution?
Specifications
| Unit Size | 0.5 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs |
| Concentration | 0.5 mg active conjugate/ml |
| Recommended Storage | 2-8 °C; Store frozen for long term storage |
| Solution | 10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 1 mg/ml bovine serum albumin. |
| Recommended Usage | The recommended concentration range for use is 2-10 μg/ml.If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. |
| Target Species | Mouse |
| Conjugate | Biotinylated |
| Host Species | Horse |
| Format | Concentrate |
Documents
- Safety Data Sheet
- Download CoA
- Datasheet
Product FAQs
I am performing IHC on tissue sections using a mouse IgG primary antibody. My detection system is a biotinylated anti-mouse IgG secondary antibody, a VECTASTAIN? Elite ABC peroxidase kit (PK-6100), in combination with a DAB substrate kit (SK-4100), and I do not see any staining. How do I know if the end detection reagents are working?
Make a fresh working solution of DAB substrate per instructions. Place a small volume (~1 mL) of this DAB substrate into a clean glass test tube. To this 1 ml aliquot, add one drop (~50 μL) of Reagent B only from the VECTASTAIN? Elite ABC kit. If the HRP enzyme is active and the DAB reacts with it, an immediate color change will be observed. This indicates the end detection reagents are working appropriately.
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Citations
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Vector Laboratories affinity-purified antibodies?are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody.?
With some exceptions, the recommended dilution for most applications is 1:100.
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