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Vector Labs 公司是世界著名的生物學(xué)檢測試劑公司，七十年代其在全球首推的生物素-親和素系統(tǒng)引領(lǐng)了生物學(xué)檢測方法的重大革命，該系統(tǒng)被普遍視為目前最靈敏、最可靠與最有效的染色系統(tǒng)，并被廣泛應(yīng)用于免疫組織化學(xué)、免疫電鏡、原位雜交與凝集素化學(xué)中。該系統(tǒng)仍在不斷地發(fā)展，以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產(chǎn)品的主要供應(yīng)商，并提供大量的新產(chǎn)品，如酶底物、神經(jīng)元示蹤劑及蛋白質(zhì)、糖類與核酸的標記、分離與檢測試劑。
Streptavidin, DyLight? 549
Description
Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.
Features:
- Highly purified and possess very low non-specific binding properties
- Excitation at 556 nm
- Emission at 571 nm
- Color:? Orange
Specifications
| Unit Size | 1 mg |
|---|---|
| Applications | Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation |
| Recommended Usage | Recommended concentration range for use5-30 μg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents.Serum also can contain biotin and should not be added to diluents. |
| Solution | 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. |
| Maximum Excitation | 556 nm |
| Maximum Emission | 571 nm |
| Recommended Storage | 2-8 °C |
| Concentration | 1.0 mg/ml active conjugate |
| Conjugate | DyLight 549 |
| Color of Fluorescence | Orange |
| Format | Concentrate |
Documents
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
VECTASHIELD? Antifade Mounting Medium Catalog #: H-1000-10
VECTASHIELD? Antifade Mounting Medium with DAPI Catalog #: H-1200-10
VECTASHIELD? HardSet™ Antifade Mounting Medium Catalog #: H-1400-10
VECTASHIELD? HardSet™ Antifade Mounting Medium with DAPI Catalog #: H-1500-10
Streptavidin/Biotin Blocking Kit Catalog #: SP-2002
Anti-Streptavidin Antibody, Biotinylated Catalog #: BA-0500-.5
Citations
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Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.
DyLight? dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight? conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight? conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.
*Additional information about the images can be found here.
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