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Streptavidin
Description
Streptavidin is a?60 kDa non-glycosylated protein composed of four identical subunits, each of which has a binding site for biotin.?Our streptavidin is isolated from Streptomyces avidinii by a specific affinity system which results in a homogeneous, ultrapure streptavidin with very low nonspecific binding characteristics.
Unconjugated streptavidin is recommended for use in solid-phase assays and immunohistochemical applications when the technique of sequential addition of reagents (sandwich technique) is employed. This reagent has also been effectively employed in subtractive hybridization procedures.
Features:
- Resembles egg white avidin in its biotin binding properties
- In most applications streptavidin and avidin are interchangeable
Specifications
| Unit Size | 1 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, ELISAs |
| Recommended Usage | Recommended method of reconstitution: dissolve in 10 mM phosphate, 0.15 M NaCl, pH 7.5. Reconstituted solutions of streptavidin may be preserved with 0.08% sodium azide. The recommended concentration range for use is 5-20 μg/ml. |
| Recommended Storage | 2-8 °C |
| Conjugate | Unconjugated |
Documents
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
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Streptavidin, AMCA Catalog #: SA-5008-1
Streptavidin, Alkaline Phosphatase Catalog #: SA-5100-1
Streptavidin, DyLight? 488 Catalog #: SA-5488-1
Streptavidin, DyLight? 549 Catalog #: SA-5549-1
Streptavidin, DyLight? 594 Catalog #: SA-5594-1
Streptavidin, DyLight? 649 Catalog #: SA-5649-1
Streptavidin, Peroxidase, R.T.U. Catalog #: SA-5704-100
Streptavidin/Biotin Blocking Kit Catalog #: SP-2002
Anti-Streptavidin Antibody, Unconjugated Catalog #: SP-4000-1
Anti-Streptavidin Antibody, Biotinylated Catalog #: BA-0500-.5
Citations
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