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Pisum Sativum Agglutinin (PSA), Biotinylated
Description
The lectin has specificity toward α-linked mannose-containing oligosaccharides, with an?N-acetylchitobiose-linked α-fucose residue included in the receptor sequence.?
Biotinylated?Pisum sativum?agglutinin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
Specifications
| Unit Size | 5 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology |
| Recommended Usage | For most applications we recommend a freshly prepared working solution of 5-20 μg/ml in the above buffer. |
| Recommended Storage | 2-8 °C; Store frozen for long term storage |
| Solution | 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 0.01 mM MnCl2, 0.08% sodium azide |
| Concentration | 5 mg active conjugate/ml |
| Conjugate | Biotinylated |
| Sugar Specificity | Mannose, Glucose |
Documents
- Lectins in Histochemistry, ELISA, and Western Blot Applications
- Safety Data Sheet
- Download CoA
- Datasheet
Product FAQs
I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?
Related Products
α-methylmannoside Catalog #: S-9005
α-methylglucoside Catalog #: S-9006
Pisum Sativum Agglutinin (PSA), Unconjugated Catalog #: L-1050-10
Glycoprotein Eluting Solution for Mannose/Glucose Binding Lectins Catalog #: ES-1100-100
Citations
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This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.
Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside