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Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Biotinylated
Description
Griffonia (Bandeiraea) Simplicifolia Lectin II is a dimeric glycoprotein composed of two subunits of nearly identical size with each subunit having disulfide-linked chains and a binding site for α- or β-linkedN-acetylglucosamine residues. Unlike other N-acetylglucosamine specific lectins, increasing the number of?N-acetylglucosamine residues beyond two does not improve affinity. GSL II has been reported to be unique in its ability to recognize exclusively α- or β-linked?N-acetylglucosamine residues on the nonreducing terminal of oligosaccharides.?
Biotinylated GSL II has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
Specifications
| Unit Size | 2 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology |
| Recommended Usage | Reconstitute biotinylated lectin by addition 1 ml of water.The resulting solution will have the following composition:10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2, 10 mM N-Acetylglucosamine.For most applications, we recommend a freshly prepared working solution of 5-20 μg/ml in the above buffer. |
| Recommended Storage | 2-8 °C; Store frozen for long term storage |
| Conjugate | Biotinylated |
| Sugar Specificity | N-Acetylglucosamine |
Documents
- Lectins in Histochemistry, ELISA, and Western Blot Applications
- Safety Data Sheet
- Download CoA
- Datasheet
Product FAQs
I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?
Related Products
Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Unconjugated Catalog #: L-1210-5
N-acetylglucosamine Catalog #: S-9002
Chitin Hydrolysate Catalog #: SP-0090-10
Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Fluorescein Catalog #: FL-1211-2
Citations
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Inhibiting/Eluting Sugar: Chitin Hydrolysate or 200 mM N-acetylglucosamine