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Vector Labs 公司是世界著名的生物學(xué)檢測(cè)試劑公司，七十年代其在全球首推的生物素-親和素系統(tǒng)引領(lǐng)了生物學(xué)檢測(cè)方法的重大革命，該系統(tǒng)被普遍視為目前最靈敏、最可靠與最有效的染色系統(tǒng)，并被廣泛應(yīng)用于免疫組織化學(xué)、免疫電鏡、原位雜交與凝集素化學(xué)中。該系統(tǒng)仍在不斷地發(fā)展，以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產(chǎn)品的主要供應(yīng)商，并提供大量的新產(chǎn)品，如酶底物、神經(jīng)元示蹤劑及蛋白質(zhì)、糖類與核酸的標(biāo)記、分離與檢測(cè)試劑。
Rabbit Anti-Goat IgG Antibody (H+L), Biotinylated
Description
The biotinylated rabbit?anti-goat?IgG?secondary antibody is?used in an avidin-biotin or streptavidin-biotin detection system.?These antibodies can be used for tissue and cell staining, ELISAs, and blots.
Features:
- Recognizes both heavy and light chains (H+L)?
- Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody?
- Included in the VECTASTAIN? ABC kits?
- Supplied in solution
- Suitable for use with goat, sheep, and bovine IgG primary antibodies
Specifications
| Unit Size | 1.5 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs |
| Concentration | 1.5 mg active conjugate/ml |
| Recommended Storage | 2-8 °C; Store frozen for long term storage |
| Solution | 10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 3 mg/ml bovine serum albumin. |
| Recommended Usage | The recommended concentration range for use is 2-10 μg/ml.If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. Important Note:The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of anti-goat IgG with bovine immunoglobulins that may be present.If this antibody is to be used in these applications, blocking with Animal-Free Blocker™ solution (Cat. No. SP-5030) is recommended. Note:This product has the same concentration as that provided in the VECTASTAIN? ABC kits. |
| Target Species | Goat |
| Conjugate | Biotinylated |
| Host Species | Rabbit |
| Format | Concentrate |
Documents
- Safety Data Sheet
- Download CoA
- Datasheet
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Citations
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Vector Laboratories affinity-purified antibodies are?are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody.?With some exceptions the recommended dilution for most applications is 1:200.
The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of this antibody with bovine immunoglobulins that may be present. If this antibody is to be used in these applications, blocking with Animal-Free Blocker? solution (Cat. No. SP-5030) is recommended.
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