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Vector Labs 公司是世界著名的生物學檢測試劑公司，七十年代其在全球首推的生物素-親和素系統(tǒng)引領(lǐng)了生物學檢測方法的重大革命，該系統(tǒng)被普遍視為目前最靈敏、最可靠與最有效的染色系統(tǒng)，并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統(tǒng)仍在不斷地發(fā)展，以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產(chǎn)品的主要供應商，并提供大量的新產(chǎn)品，如酶底物、神經(jīng)元示蹤劑及蛋白質(zhì)、糖類與核酸的標記、分離與檢測試劑。
M.O.M.? (Mouse on Mouse) Immunodetection Kit, Basic
Description
The M.O.M. Basic?Kit?is?useful in any mouse-on-mouse application, such as studies in genetically engineered mice, including transgenic and knock-out models, as well as mouse xenografts. ?The M.O.M. Kit can be also used in multiple antigen staining protocols to localize two different mouse primary antibodies on the same mouse tissue sections using fluorescent or enzyme-based detection systems.?
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Key advantages of the Vector M.O.M. Immunodetection Kits:
- Significant reduction of endogenous mouse Ig staining when using mouse primary antibodies on mouse tissue
- Clear, crisp specific staining of antigens of interest
- Based on VECTASTAIN? ABC reagent protocols
- No tedious calculations or antibody prebinding steps required
- Contains enough stock reagents to produce about 25 ml of working solution, which is generally sufficient to stain approximately 250 tissue sections.
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Specifications
| Unit Size | 1 kit |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence |
| Technology | ABC (Avidin/Biotin) Systems, Mouse on Mouse (M.O.M.) Systems |
| Target Species | Mouse |
| Format | Concentrate |
| Blocking Action | Endogenous Mouse Ig |
Kit Components
Kit Contents:
- 6 ml of M.O.M. Protein Concentrate
- 1 ml M.O.M. Mouse Ig Blocking Reagent
- 0.1 ml M.O.M. Biotinylated Anti-Mouse IgG Reagent
Documents
- M.O.M. (Mouse on Mouse) Troubleshooting Guide
- User Guide
- Protocol for Multiple Immunofluorescent Staining Using Mouse Antibodies
- Safety Data Sheet
- Download CoA
- Datasheet
Product FAQs
For my IHC application, I intend to apply a mouse IgG primary antibody on rat tissue sections. Should I use the Vector Mouse on Mouse detection kit?
Related Products
Avidin/Biotin Blocking Kit Catalog #: SP-2001
M.O.M.? (Mouse on Mouse) Biotinylated Anti-Mouse IgG Reagent Catalog #: MKB-2225-.1
M.O.M.? (Mouse on Mouse) Blocking Reagent Catalog #: MKB-2213-1
VECTASTAIN? Elite ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-6100
VECTASTAIN? ABC-AP Kit, Alkaline Phosphatase (Standard) Catalog #: AK-5000
Streptavidin, DyLight? 594 Catalog #: SA-5594-1
Streptavidin, DyLight? 488 Catalog #: SA-5488-1
Citations
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Four M.O.M. Kits are available. The three kits based on avidin/biotin technology use the same proprietary M.O.M. Mouse Ig Blocking Reagent and the M.O.M. Biotinylated Anti-Mouse Ig Reagent, but offer a choice of an enzyme-based or fluorescent-based visualization method. M.O.M. Mouse Ig Blocking Reagent and M.O.M. Biotinylated Anti-Mouse Ig Reagent are also available separately.
The M.O.M. ImmPRESS? Kit contains our proprietary M.O.M. Mouse Ig Blocking Reagent paired with a specialized, ready-to-use, one-step, non-biotin M.O.M. ImmPRESS Peroxidase Polymer reagent.
An enzyme or fluorochrome conjugate is not included allowing for a choice of any avidin or streptavidin-based detection system. Choose any peroxidase or alkaline phosphatase conjugated avidin or streptavidin for chromogenic detection (for example, VECTASTAIN?ABC-AP Standard Kit followed by a Vector alkaline phosphatase substrate), or choose a fluorochrome-labeled avidin or streptavidin.
Product review on Biocompare.com.