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Lotus Tetragonolobus Lectin (LTL), Biotinylated
Description
Lotus tetragonolobus?lectin is a family of closely related glycoproteins that appear to have similar specificities toward α-linked L-fucose containing oligosaccharides. Although many of the binding properties of Lotus lectin are similar to those of Ulex europaeus lectin I, the binding affinities and some specificities for oligosaccharides are significantly different between these fucose-specific lectins.
Biotinylated?Lotus tetragonolobus?has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
Specifications
| Unit Size | 2 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology |
| Recommended Usage | Reconstitute by adding 1ml water.The resulting solution will have the following composition:10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2.For most applications, we recommend a freshly prepared working solution of5-20 μg/ml in the above buffer. |
| Recommended Storage | 2-8 °C; Store frozen for long term storage |
| Conjugate | Biotinylated |
| Sugar Specificity | Fucose, Arabinose |
Documents
- Lectins in Histochemistry, ELISA, and Western Blot Applications
- Safety Data Sheet
- Download CoA
- Datasheet
Product FAQs
I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?
Related Products
Lotus Tetragonolobus Lectin (LTL), Unconjugated Catalog #: L-1320-5
L-fucose Catalog #: S-9007
Lotus Tetragonolobus Lectin (LTL), Fluorescein Catalog #: FL-1321-2
Citations
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Inhibiting/Eluting Sugar: 50 mM - 100 mM L-fucose