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ImmPACT? SG Substrate, Peroxidase (HRP)
Description
The?ImmPACT SG HRP substrate produces a blue-gray reaction product?that can be used as a single label or as a second color for multiple antigen labeling. It is?2-4 fold greater sensitivity than original substrate kit.
Features:
- Optimized for IHC/ICC/ISH and blots
- Permanent mounting
- Working solutions stable for up to 14 days at 4 °C
- Supplied as 120 ml of diluent and stock solutions in dropper bottles
Specifications
| Unit Size | 120 ml |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot |
| Detection Enzyme(s) | Peroxidase |
| Substrate Reaction Product | Precipitating Reaction Product |
| Chromogen Color | Blue-Gray |
| Microscopy | Brightfield, Darkfield, Electron, Spectral Imaging |
| Additional Substrate Properties | Contrast with Tissue Pigments, Multiple Labeling |
| Mounting | Aqueous (Hardening), Non-Aqueous (Permanent) |
Kit Components
Kit Contents:
- 120 ml ImmPACT SG Diluent
- 2.5 ml ImmPACT SG Reagent 1
- 3 ml ImmPACT SG Reagent 2
Documents
- Enzyme Substrates Brochure
- Enzyme Substrate Combinations Chart
- Counterstain/Substrate Compatibility Chart
- User Guide
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
BLOXALL? Endogenous Blocking Solution, Peroxidase and Alkaline Phosphatase Catalog #: SP-6000-100
Nuclear Fast Red Counterstain Catalog #: H-3403-500
Methyl Green Counterstain Catalog #: H-3402-500
VectaMount? Permanent Mounting Medium Catalog #: H-5000-60
VectaMount? AQ Aqueous Mounting Medium Catalog #: H-5501-60
Citations
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Sections stained with the substrate also can be viewed by darkfield or electron microscopy. Vector SG and ImmPACT SG substrates can be used for both manual and automated staining methods. Stained sections can be dehydrated, cleared, and permanently mounted. This substrate formulation can be used on nylon or PVDF membranes but is not recommended for use on nitrocellulose membranes.
