Vector Labs代理，歡迎訪問Vector Labs官網(wǎng)或者咨詢我們獲取相關(guān)Vector Labs產(chǎn)品信息以及報價。
Vector Labs 公司是世界著名的生物學(xué)檢測試劑公司，七十年代其在全球首推的生物素-親和素系統(tǒng)引領(lǐng)了生物學(xué)檢測方法的重大革命，該系統(tǒng)被普遍視為目前最靈敏、最可靠與最有效的染色系統(tǒng)，并被廣泛應(yīng)用于免疫組織化學(xué)、免疫電鏡、原位雜交與凝集素化學(xué)中。該系統(tǒng)仍在不斷地發(fā)展，以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產(chǎn)品的主要供應(yīng)商，并提供大量的新產(chǎn)品，如酶底物、神經(jīng)元示蹤劑及蛋白質(zhì)、糖類與核酸的標(biāo)記、分離與檢測試劑。
Fluorescein Avidin DCS, Cell Sorting Grade
Description
Avidin DCS, Fluorescein labeled, is a cell sorter grade of Fluorescein Avidin D. Through the development of a new method of fluorescein labeling we are able to incorporate more fluorochrome into a protein without altering the properties of the protein. Not only does the Fluorescein Avidin DCS have an optimal F/P ratio, the efficiency of fluorescence is also increased. In addition to the improved conjugation technique, we employ a purification step to reduce nonspecific binding which can hamper resolution in cell sorting and other applications.?
Specifications
| Unit Size | 1 mg |
|---|---|
| Applications | Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation |
| Recommended Usage | For staining cells or tissues using fluorescent microscopy, the recommended concentration range is 5-30 μg/ml with incubation times of 15-30 minutes. |
| Solution | 10 mM HEPES, 0.5 M NaCl, pH 8.0, 0.08% sodium azide, 10 mg/ml beta-cyclodextrin |
| Maximum Excitation | 495-500 nm |
| Maximum Emission | 514-521 nm |
| Recommended Storage | 2-8 °C |
| Concentration | 2.0 mg active conjugate/ml |
| Conjugate | Fluorescein |
| Color of Fluorescence | Green |
Documents
- Double Immunofluorescent Labeling Protocol
- Fluorescence Detection of Biotin-Labeled ISH Probes – Protocol
- Fluorescence Detection of Fluorescein-Labeled ISH Probes – Protocol
- In Situ Hybridization Detection Protocol
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
Avidin/Biotin Blocking Kit Catalog #: SP-2001
Anti-Avidin Antibody, Biotinylated Catalog #: BA-0300-.5
VECTASHIELD? Antifade Mounting Medium Catalog #: H-1000-10
VECTASHIELD? Antifade Mounting Medium with DAPI Catalog #: H-1200-10
VECTASHIELD? Antifade Mounting Medium with Propidium Iodide (PI) Catalog #: H-1300-10
VECTASHIELD? HardSet™ Antifade Mounting Medium Catalog #: H-1400-10
VECTASHIELD? HardSet™ Antifade Mounting Medium with DAPI Catalog #: H-1500-10
VECTASHIELD? HardSet™ Antifade Mounting Medium with Phalloidin Catalog #: H-1600-10
Citations
Powered by Bioz See more details on BiozTechnical Information
Although this product has been designed for cell sorting, it can also be used in fluorescent microscopy when higher sensitivity and lower background staining are necessary. This derivative is preferred when using the amplification procedure with Biotinylated Anti-Avidin D.?
- Excitation maximum:? 495 nm
- Emission maximum:? 515 nm
- Color:? Green