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Vector Labs 公司是世界著名的生物學檢測試劑公司，七十年代其在全球首推的生物素-親和素系統(tǒng)引領了生物學檢測方法的重大革命，該系統(tǒng)被普遍視為目前最靈敏、最可靠與最有效的染色系統(tǒng)，并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統(tǒng)仍在不斷地發(fā)展，以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產品的主要供應商，并提供大量的新產品，如酶底物、神經元示蹤劑及蛋白質、糖類與核酸的標記、分離與檢測試劑。
Goat Anti-Human IgG, gamma chain specific, Fluorescein
Description
Fluorescein Goat Anti-Human IgG, gamma chain specific,?binds to human?primary antibodies for?immunohistochemistry and many?other applications.?This gamma chain specific antibody has virtually no cross-reactivity with other immunoglobulin classes or other heavy or light chains.
Features:
- Affinity-purified, ultrapure, high affinity antibody?
- Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species?
- Optimally labeled with fluorescein to provide the brightest label for fluorescence microscopy?
- Supplied in solution?
- Excitation: 495 nm?
- Emission: 515 nm?
- Color: Green
- This chain-specific antibody is produced specifically to distinguish between chains or classes of target immunoglobulins.?
Specifications
| Unit Size | 0.5 mg |
|---|---|
| Applications | Immunofluorescence, Blotting Applications, Flow Cytometry/Cell Separation |
| Concentration | 1.0 mg active conjugate/ml |
| Recommended Storage | 2-8 °C |
| Solution | 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. |
| Maximum Emission | 510-520 nm |
| Maximum Excitation | 490-500 nm |
| Recommended Usage | The recommended concentration range for use is 5-20 μg/ml. If this fluorescein-labeled antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2% normal serum from the same species as the tissue. |
| Target Species | Human |
| Conjugate | Fluorescein |
| Color of Fluorescence | Green |
| Host Species | Goat |
| Format | Concentrate |
Documents
- Safety Data Sheet
- Download CoA
- Datasheet
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Citations
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The goat anti-human Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis.?
This antibody can be used for flow cytometry or for tissue staining. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.?
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