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Vector Labs 公司是世界著名的生物學檢測試劑公司，七十年代其在全球首推的生物素-親和素系統(tǒng)引領(lǐng)了生物學檢測方法的重大革命，該系統(tǒng)被普遍視為目前最靈敏、最可靠與最有效的染色系統(tǒng)，并被廣泛應(yīng)用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統(tǒng)仍在不斷地發(fā)展，以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產(chǎn)品的主要供應(yīng)商，并提供大量的新產(chǎn)品，如酶底物、神經(jīng)元示蹤劑及蛋白質(zhì)、糖類與核酸的標記、分離與檢測試劑。
Horse Anti-Mouse/Rabbit/Goat IgG Antibody (H+L) (Universal Pan-Specific ), Biotinylated
Description
This pan-specific, biotinylated universal secondary antibody is specially formulated to recognize IgG (H+L) primary antibodies from mouse, rabbit, or goat. This mixture of biotinylated secondary antibodies is produced in horse, and provides the same high sensitivity and low background as that obtained with our species-specific biotinylated anti-mouse IgG (H+L), biotinylated anti-rabbit IgG (H+L), or biotinylated anti-goat IgG (H+L). Use of this pan-specific secondary antibody provides added convenience and is adaptable to automated instrument staining.
Features:
- Recognizes both heavy and light chains (H+L)?
- Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody?
- Can be used for tissue and cell staining, ELISAs, and blots?
- Included in the VECTASTAIN? Universal Quick kits
- Supplied as 2.2 ml of solution
- Recommended dilution for most applications is 1:20
- This pan-specific, biotinylated “universal” secondary antibody is specially formulated to recognize IgG (H+L) primary antibodies from mouse, rabbit, or goat.
Note: This antibody has significant cross-reactivity for rodent, rabbit, goat, sheep and bovine IgG and is not recommended for use in staining tissues of these species.
Note: This product is the same biotinylated secondary antibody included in the VECTASTAIN??Universal Quick Kit, PK-8800, but is not the same as that provided in the VECTASTAIN??Elite ABC Kit (Universal), PK-6200.
Specifications
| Unit Size | 2.2 ml |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs |
| Concentration | 0.91 mg/ml |
| Recommended Storage | 2-8 °C; Store frozen for long term storage |
| Solution | 10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 3 mg/ml bovine serum albumin. |
| Recommended Usage | NOTE:This product is a specially prepared biotinylated secondary antibody which recognizes primary antibodies made in mouse, rabbit or goat.This antibody should not be used on tissues from mouse, rabbit or goat or from animals related immunologically to these species.Significant background due to crossreactivity with endogenous immunoglobulins may result from its inappropriate use.This product is the same as that included in the VECTASTAIN? Universal Quick Kit (Cat. No. PK-8800)but is not the same as the antibody included in the VECTASTAIN? Universal Elite? ABC Kit. If this biotinylated antibody is to be used in a 30 minute incubation, dispense 4 drops into 5 ml of phosphate buffered saline (PBS) containing 1.5% normal horse serum. If this biotinylated antibody is to be used in a 10 minute incubation, dispense 1 drop per ml of PBS containing 5-10% normal horse serum. |
| Target Species | Universal (Mouse/Rabbit/Goat) |
| Conjugate | Biotinylated |
| Host Species | Horse |
| Format | Concentrate |
Documents
- Safety Data Sheet
- Download CoA
- Datasheet
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Citations
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Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody.
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