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Vector? TrueVIEW? Autofluorescence Quenching Kit with DAPI
Description
The TrueVIEW Autofluorescence Quenching Kit with DAPI provides a novel way?to diminish unwanted autofluorescence from non-lipofuscin sources and dramatically improve signal-to-noise ratio. It removes unwanted autofluorescence in tissue sections due to aldehyde fixation, red blood cells, and structural elements such as collagen and elastin.?The quenching action of the kit reagents provides a clear, unambiguous, “true view” localization of the target antigen.
Features:
- Effective on problematic tissues such as kidney and spleen
- Easy-to-use, one-step method
- Compatible with a wide selection of fluorophores
- Compatible with standard epifluorescence and confocal laser microscopes
- Mounting medium contains DAPI for counterstaining in one step
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One kit is sufficient to treat approximately 100 to 150 tissue sections.?
Specifications
| Unit Size | 15 ml |
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| Blocking Action | Autofluorescence |
Kit Components
Kit Contents:
- TrueVIEW Reagent A, 5 ml
- TrueVIEW Reagent B, 5 ml
- TrueVIEW Reagent C, 5 ml
- VECTASHIELD? Vibrance™ Antifade Mounting Medium with DAPI, 2 ml
Documents
- Safety Data Sheet
- TrueVIEW? Application Note
- User Guide
- Download CoA
- Datasheet
Product FAQs
Why is the TrueVIEW™ Autofluorescence Quenching reagent applied after completion of the IF assay and not at the start of the procedure?
Do you have any published references describing the use of TrueVIEW Autofluorescence Quenching Kit?
Yes, there are a number of published references describing the use of TrueVIEW Autofluorescence Quenching Kit in the scientific literature. Please refer to a partial list of these publications in the Technical Information section of the product detail page for SP-8400.
Is TrueVIEW effective against lipofuscin derived autofluorescence?
How long can I store the TrueVIEW working solution?
My tissue sections turned blue when I applied TrueVIEW. Is this supposed to happen?
What mounting media can I use with TrueVIEW?
For the wash step after applying the TrueVIEW quenching reagent, can I use buffers other than PBS or detergents?
No, we have found that the TrueVIEW reagent lifts off the tissue using TBS or HEPES buffer. Detergents are incompatible.
Technical Information
Tissue autofluorescence often occurs with aldehyde fixation or from inherent native tissue components (collagen, elastin, and red blood cells). The extent and intensity of autofluorescence background frequently?makes?it difficult or impossible to distinguish specific signals in immunofluorescence applications.
Most methods for reduction of tissue autofluorescence act primarily on lipofuscin granules, and are not broadly effective against the most common sources of autofluorescence targeted by TrueVIEW Quencher.
Current methods for reducing autofluorescence primarily include “home brew” concoctions such as sodium borohydride and other ink-based products. These methods are essentially ineffective against aldehyde induced autofluorescence. ?In contrast, Vector?TrueVIEW reagent binds to hydrophilic compounds and effectively quenches endogenous autofluorescence.?
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Human tonsil (FFPE); Section stained for AE1/AE3 using fluorescein label (green). Treated with TrueVIEW and mounted with VECTASHIELD Vibrance Antifade Mounting Medium with DAPI (nuclei blue).? |
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Easy To ApplyFollowing completion of the IF staining procedure: |
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Mode of ActionFollowing completion of the IF staining procedure: |
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Signal to Noise OptimizationThe primary antibody and detection reagents should be optimized (titered) in conjunction with Vector?TrueVIEW Quenching Kit to achieve maximum signal to noise.? |
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Optimization of ExposureIncreasing exposure times may be necessary to achieve optimal image acquisition for?TrueVIEW Quencher treated slides. |
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Comparison Between Vector?TrueVIEW Reagent and Other Autofluorescence Reducing AgentsWe compared the effectiveness of Vector?TrueVIEW quenching action in parallel with other commercially available autofluorescence reducing products and “home brew” reagents, on serial sections of formalin-fixed, paraffin-embedded human pancreas visualized using a standard fluorescein (green) filter. No specific immunofluorescence staining was conducted. The images below highlight our results. All images were acquired under identical conditions (including microscope objective and exposure times).? |
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Customer Testimonials? |
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