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VectaMount? Express Mounting Medium
Description
VectaMount??Express is a?non-aqueous?clearing and?mounting medium?enabling the rapid?mounting?of?cell and tissue specimens following?immunohistochemistry (IHC)?staining. This novel formulation is engineered to enable mounting directly following staining, saving time by eliminating the need for extensive ethanol and clearing washes prior to?coverslipping. Just stain your slides as per your usual workflow, briefly wash in isopropyl alcohol, and?coverslip.?
Features:
- Non-aqueous?clearing and?mounting?medium?for IHC-stained?slides
- Eliminates?the need for extensive?ethanol?washes and?solvent-based?clearing agents (e.g.,?xylene)
- Rapid drying?formula?for fast visualization of stained samples
- Preserves staining for at least 18 months at room temperature
- Compatible with?horseradish peroxidase (HRP) and?alkaline phosphatase (AP)?enzyme?substrates, as well as commonly used counterstains
- Optically clear formula with a refractive index of 1.49, resulting in high-quality imaging?free of distortion or artifact
Specifications
| Unit Size | 60 ml |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, In situ hybridization |
| Recommended Usage | VectaMount Express Mounting Medium is a?non-aqueous?clearing and?mounting medium?enabling the rapid?mounting?of?cell and tissue specimens following?IHC?staining.?VectaMount Express is compatible with HRP and AP?enzyme?substrates, as well as commonly used counterstains. |
| Recommended Storage | Room Temperature |
| Mounting | Non-Aqueous |
| Antifade | No |
| Safety Title | WARNING: Reproductive Harm – www.P65Warnings.ca.gov |
Documents
- User Guide
- Safety Data Sheet
- Substrate Compatibility Table
- Download CoA
- Datasheet
Product FAQs
After mounting with VectaMount Express, why do my slides look cloudy?
It is important to use 99+% alcohol (isopropanol or ethanol) for the brief dehydration steps. Lower alcohol grades will not be viable substitutes. Slides should be rinsed in tap water (not buffer) prior to dehydration, as salts in buffers can precipitate if not removed. Depending on slide volume, alcohol baths should be changed out on a regular basis with fresh solution. It is suggested to drain excess water when transferring slides from tap water into the alcohol bath.
Why am I getting air bubbles under the coverslip after mounting with Vectamount Express?
Usually the presence of air bubbles can be greatly reduced, and even eliminated, when using a manual approach, by carefully lowering the coverslip slowly over the specimen. Sometimes this may take a little practice to perfect.
Can I dry the Vectamount Express mounted slides in an oven?
No. It is recommended to dry slides at ambient room temperature.
How long can the slides remain in isopropanol before mounting with Vectamount Express?
There are no specific time limits for slides to be in the alcohol bath. Depending on the number of slides in a given assay, the last slide to be mounted will be in the alcohol bath longer than the first mounted slide. This may take several minutes or much longer. We have not seen any observable difference in specimen characteristics, staining or media performance with longer alcohol exposure times.
How can I successfully remove the coverslip once it has been applied to a slide with VectaMount Express Mounting Medium?
Place the slides in a glass staining rack or Coplin jar and completely submerge the slides in xylene overnight. After this time the coverslip should be easy to remove from the slide.
Is VectaMount Express Mounting Medium compatible with counterstains?
Yes. From our in-house testing, commonly used counterstains such as hematoxylin (e.g. Gill’s and Mayers formulations), nuclear fast red, methyl green and standard H&E (hematoxylin and eosin) stains are compatible with VectaMount Express Mounting Medium.
Related Products
VectaMount? AQ Aqueous Mounting Medium Catalog #: H-5501-60
VectaMount? Permanent Mounting Medium Catalog #: H-5000-60
Technical Information
VectaMount Express Mounting Medium is a?non-aqueous?clearing and?mounting medium?enabling the rapid?mounting?of?cell and tissue specimens, without the need for slide handling through an ethanol dehydration series and xylene clearing prior to coverslipping. VectaMount Express Mounting Medium preserves the color and intensity of preparations stained with enzyme substrates, such as DAB and BCIP/NBT, as well as our proprietary substrates Vector? NovaRED™ , Vector VIP, Vector SG, ImmPACT? DAB, ImmPACT DAB EqV, ImmPACT NovaRED, ImmPACT VIP, ImmPACT SG, Vector Red, ImmPACT Vector Red, and Vector Blue, for at least 18 months at room temperature. With a refractive index (1.49)?closely matching that of the slide and tissue?specimen, this mounting medium results in high-quality imaging?free of distortion or artifact.
Preserves staining for at least 18 months
