Vector Labs代理，歡迎訪問Vector Labs官網(wǎng)或者咨詢我們獲取相關(guān)Vector Labs產(chǎn)品信息以及報價。
Vector Labs 公司是世界著名的生物學檢測試劑公司，七十年代其在全球首推的生物素-親和素系統(tǒng)引領(lǐng)了生物學檢測方法的重大革命，該系統(tǒng)被普遍視為目前最靈敏、最可靠與最有效的染色系統(tǒng)，并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統(tǒng)仍在不斷地發(fā)展，以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產(chǎn)品的主要供應商，并提供大量的新產(chǎn)品，如酶底物、神經(jīng)元示蹤劑及蛋白質(zhì)、糖類與核酸的標記、分離與檢測試劑。
Wisteria Floribunda Lectin (WFA, WFL), Fluorescein
Description
The binding specificity of?Wisteria floribunda?lectin (WFL) is not completely clear but this lectin appears to preferentially bind carbohydrate structures terminating in?N-acetylgalactosamine linked α or β to the 3 or 6 position of galactose. This lectin has been used to fractionate lymphocyte populations, and although not mitogenic, elicits the production of lymphokines from murine splenocytes.
Fluorescein labeled WFL has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.
- Excitation maximum: 495 nm
- Emission maximum:?515 nm
- Color: Green
Specifications
| Unit Size | 2 mg |
|---|---|
| Applications | Immunofluorescence, Glycobiology |
| Recommended Usage | The recommended concentration range for use is 5-20 μg/ml. |
| Recommended Storage | 2-8°C |
| Maximum Excitation | 495-500 nm |
| Inhibiting and/or Eluting Sugar | 200 mM N-acetylgalactosamine(S-9001) |
| Maximum Emission | 514-521 nm |
| Solution | 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. 0.1 mM CaCl2 |
| Concentration | 2 mg active conjugate/ml |
| Conjugate | Fluorescein |
| Color of Fluorescence | Green |
| Sugar Specificity | N-Acetylgalactosamine |
Documents
- Lectins in Histochemistry, ELISA, and Western Blot Applications
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
Wisteria Floribunda Lectin (WFA, WFL), Biotinylated Catalog #: B-1355-2
Wisteria Floribunda Lectin (WFA, WFL), Unconjugated Catalog #: L-1350-5
N-acetylgalactosamine Catalog #: S-9001
Wisteria Floribunda Lectin (WFA, WFL), Agarose bound Catalog #: AL-1353-2
Citations
Powered by Bioz See more details on BiozTechnical Information
Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine