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Phaseolus Vulgaris Leucoagglutinin (PHA-L), Unconjugated
Description
Phaseolus vulgaris?agglutinin is the name ascribed to a family of lectins, each of which consists of four subunits. There are two different types of subunits. One appears to be involved primarily in red cell agglutination and has been designated the “E” subunit (for erythroagglutinin). The other type is involved in lymphocyte agglutination and mitogenic activity and has been termed the “L” subunit (for leucoagglutinin). These subunits combine to produce five isolectins.?
The unconjugated PHA-L has been found to be an excellent, specific marker for use in anterograde neuronal tracing.
Specifications
| Unit Size | 5 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology, Mitogenic Stimulation |
| Recommended Usage | Reconstitution: The lectin should be reconstituted 10 mM phosphate, pH 8.0. If reconstituted in 1 ml of 10 mM phosphate, the resulting solution will be 5 mM phosphate, 10 mM NaCl with trace CaCl2. After reconstitution, and if appropriate for use, a preservative such as sodium azide (0.04%) may be added. If the lectin is to be used for mitogenic assays, filter solution through a sterile 0.22 μm filter immediately after reconstitution, aliquot and store frozen. NOTE: Mitogenic activity may be diminished by repeated freezing and thawing. Anterograde Neuronal Tracing: For neuronal transport studies, reconstitute in 0.2 ml of 10 mM sodium phosphate, pH 8.0.Both the pH and ionic strength of the reconstituting buffer are important for optimal anterograde transport. A specific protocol for this application is available on request. |
| Recommended Storage | 2-8 °C |
| Conjugate | Unconjugated |
| Sugar Specificity | Galactose, Complex Structures |
Documents
- PHA-L METHOD FOR TRACING EFFERENT NEURONAL PROJECTIONS
- Lectins in Histochemistry, ELISA, and Western Blot Applications
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
Phaseolus Vulgaris Leucoagglutinin (PHA-L), Biotinylated Catalog #: B-1115-2
Phaseolus Vulgaris Leucoagglutinin (PHA-L), Fluorescein Catalog #: FL-1111-2
VECTASTAIN? Elite ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-6100
Phaseolus Vulgaris Leucoagglutinin (PHA-L), Rhodamine Catalog #: RL-1112-2
ImmPACT? DAB Substrate, Peroxidase (HRP) Catalog #: SK-4105
DAB Substrate Kit, Peroxidase (HRP), with Nickel, (3,3'-diaminobenzidine) Catalog #: SK-4100
Rabbit Anti-Phaseolus Vulgaris Agglutinin (E+L), Unconjugated Catalog #: AS-2300-1
Goat Anti-Phaseolus Vulgaris Agglutinin (E+L), Unconjugated Catalog #: AS-2224-1
Citations
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Elution: 100 mM acetic acid