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Vector Labs 公司是世界著名的生物學(xué)檢測(cè)試劑公司，七十年代其在全球首推的生物素-親和素系統(tǒng)引領(lǐng)了生物學(xué)檢測(cè)方法的重大革命，該系統(tǒng)被普遍視為目前最靈敏、最可靠與最有效的染色系統(tǒng)，并被廣泛應(yīng)用于免疫組織化學(xué)、免疫電鏡、原位雜交與凝集素化學(xué)中。該系統(tǒng)仍在不斷地發(fā)展，以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產(chǎn)品的主要供應(yīng)商，并提供大量的新產(chǎn)品，如酶底物、神經(jīng)元示蹤劑及蛋白質(zhì)、糖類與核酸的標(biāo)記、分離與檢測(cè)試劑。
Glycoprotein Eluting Solution for Mannose/Glucose Binding Lectins
Description
Vector Laboratories has developed Glycoprotein Elution Solutions in the neutral pH range that maximize the yield of eluted glycoproteins and preserve the activity of the agarose-bound lectins for repeated use. All components of these ready-to-use Glycoprotein Eluting Solutions can be subsequently removed by dialysis. These Glycoprotein Eluting Solutions offer researchers convenience and superior recovery over standard sugar solutions.
Features:
- Preserve the activity of the agarose-bound lectins for repeated use
- Elution in neutral pH range
Specifications
| Unit Size | 100 ml |
|---|---|
| Applications | Glycobiology, Affinity Chromatography |
| Recommended Usage | 1)Wash lectin agarose column to remove unbound proteins with HEPES- or TRIS-Buffered saline (HBS, TBS), pH 7.5, until OD280nm reaches background level. 2)Apply Glycoprotein Eluting Solution and allow to flow through the column by gravity.Note: Do not apply pressure to speed elution because this may reduce the percent recovery of the bound glycoprotein.Complete elution may take up to 10 column volumes.* 3) Following elution, the agarose lectin column is ready for reuse after equilibrating with 2-4 column volumes of HBS or TBS, pH 7.5. *These results may not be typical for other glycoproteins, since oligosaccharide structures of glycoproteins vary significantly.The eluting solution has a high osmolarity.If downstream applications are adversely affected by salt, eluted glycoproteins may require dialysis or gel filtration. |
| Recommended Storage | Room Temperature |
Documents
- Safety Data Sheet
- Download CoA
- Datasheet
Product FAQs
What is the primary composition of the Vector Labs glycoprotein eluting solutions? Do any of these contain protein?
Related Products
Glycoprotein Eluting Solution for GlcNAc/Chitin Binding Lectins Catalog #: ES-5100-100
Glycoprotein Eluting Solution for Sialic Acid Binding Lectins Catalog #: ES-7100-100
Concanavalin A (Con A), Agarose bound Catalog #: AL-1003
Galanthus Nivalis Lectin (GNL), Agarose bound Catalog #: AL-1243-5
Glycoprotein Eluting Solution for Galactose/GalNAc Binding Lectins Catalog #: ES-2100-100
Technical Information
