Vector Labs代理，歡迎訪問(wèn)Vector Labs官網(wǎng)或者咨詢(xún)我們獲取相關(guān)Vector Labs產(chǎn)品信息以及報(bào)價(jià)。
Vector Labs 公司是世界著名的生物學(xué)檢測(cè)試劑公司，七十年代其在全球首推的生物素-親和素系統(tǒng)引領(lǐng)了生物學(xué)檢測(cè)方法的重大革命，該系統(tǒng)被普遍視為目前最靈敏、最可靠與最有效的染色系統(tǒng)，并被廣泛應(yīng)用于免疫組織化學(xué)、免疫電鏡、原位雜交與凝集素化學(xué)中。該系統(tǒng)仍在不斷地發(fā)展，以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產(chǎn)品的主要供應(yīng)商，并提供大量的新產(chǎn)品，如酶底物、神經(jīng)元示蹤劑及蛋白質(zhì)、糖類(lèi)與核酸的標(biāo)記、分離與檢測(cè)試劑。
Galanthus Nivalis Lectin (GNL), Biotinylated
Description
Galanthus nivalis?lectin, unlike most mannose-specific lectins, is not a metalloprotein and does not require Ca++?or Mn++?for binding.?
Binding seems to be preferentially directed toward structures containing (α-1,3) mannose residues. Also in contrast to most mannose-binding lectins, GNL will not bind α-linked glucose. Reports indicate that this lectin binds rat and mouse IgM but not IgG. The only protein from human serum reported to bind to this lectin is α2-macroglobulin. GNL binds to many viral glycoproteins.?
Biotinylated?Galanthus nivalis?lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
Specifications
| Unit Size | 2 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology |
| Recommended Usage | For most applications, we recommend a freshly prepared working solution of 5-20 μg/ml in the above buffer. A precipitate may form during storage.This does not have a significant adverse effect on the product.If a precipitate forms upon long-term storage, warm to 37 oC and centrifuge before use. |
| Recommended Storage | 2-8 °C; Store frozen for long term storage |
| Solution | 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2. |
| Concentration | 2 mg/ml |
| Conjugate | Biotinylated |
| Sugar Specificity | Mannose |
Documents
- Lectins in Histochemistry, ELISA, and Western Blot Applications
- Safety Data Sheet
- Download CoA
- Datasheet
Product FAQs
I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?
Related Products
α-methylmannoside Catalog #: S-9005
Galanthus Nivalis Lectin (GNL), Unconjugated Catalog #: L-1240-5
Galanthus Nivalis Lectin (GNL), Fluorescein Catalog #: FL-1241-2
Citations
Powered by Bioz See more details on BiozTechnical Information
Inhibiting/Eluting Sugar: 100 mM - 200 mM α-methylmannoside