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Vector Labs 公司是世界著名的生物學(xué)檢測試劑公司，七十年代其在全球首推的生物素-親和素系統(tǒng)引領(lǐng)了生物學(xué)檢測方法的重大革命，該系統(tǒng)被普遍視為目前最靈敏、最可靠與最有效的染色系統(tǒng)，并被廣泛應(yīng)用于免疫組織化學(xué)、免疫電鏡、原位雜交與凝集素化學(xué)中。該系統(tǒng)仍在不斷地發(fā)展，以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產(chǎn)品的主要供應(yīng)商，并提供大量的新產(chǎn)品，如酶底物、神經(jīng)元示蹤劑及蛋白質(zhì)、糖類與核酸的標(biāo)記、分離與檢測試劑。
ImmPACT? VIP Substrate, Peroxidase (HRP)
Description
The ImmPACT?VIP?HRP substrate?produces?an?intense, violet (purple) reaction product, and can be used as an alternative to DAB or as a second color for multiple antigen labeling.?This product?generates an intense reaction product that is 2-4 times more sensitive than the?Vector VIP substrate kit (SK-4600).
Features:
- 2-4 fold greater sensitivity than original substrate kit
- Optimized for IHC/ICC/ISH and blots
- Permanent mounting
- Ideal for single and multiple labeling
- Working solutions stable for up to 14 days at 4 °C
- Supplied as 120 ml of diluent and stock solutions in dropper bottles
?
Specifications
| Unit Size | 120 ml |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot |
| Detection Enzyme(s) | Peroxidase |
| Substrate Reaction Product | Precipitating Reaction Product |
| Chromogen Color | Purple |
| Microscopy | Brightfield, Darkfield, Electron, Spectral Imaging |
| Additional Substrate Properties | Contrast with Tissue Pigments, Multiple Labeling |
| Mounting | Non-Aqueous (Permanent) |
Kit Components
Kit Contents:
- 120 ml ImmPACT VIP Diluent
- 3 ml ImmPACT VIP Reagent 1
- 2.5 ml ImmPACT VIP Reagent 2
- 2.5 ml ImmPACT VIP Reagent 3
- 3 ml ImmPACT VIP Reagent 4
Documents
- Enzyme Substrates Brochure
- Enzyme Substrate Combinations Chart
- Counterstain/Substrate Compatibility Chart
- User Guide
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
BLOXALL? Endogenous Blocking Solution, Peroxidase and Alkaline Phosphatase Catalog #: SP-6000-100
Methyl Green Counterstain Catalog #: H-3402-500
Hematoxylin Counterstain Catalog #: H-3401-500
Hematoxylin QS Counterstain Catalog #: H-3404-100
VectaMount? Permanent Mounting Medium Catalog #: H-5000-60
Citations
Powered by Bioz See more details on BiozTechnical Information
Vector VIP and ImmPACT VIP chromogens also provide excellent color contrast in pigmented tissues such as melanoma or retina (1). Sections stained with either substrate can be viewed by darkfield and electron microscopy. Vector VIP and ImmPACT VIP substrates can be used for both manual and automated staining methods. Stained sections should be dehydrated, cleared, and permanently mounted.
This kit consists of 120 ml of diluent and concentrated stock solutions of ImmPACT VIP reagents in convenient dropper bottles. Working solutions are stable for up to 14 days at 4 °C.
Product review on Biocompare.com.
1. Duncan SM, Seigel GM. High-contrast enzymatic immunohistochemistry of pigmented tissues. J Biol Methods2016;3(3):e47. doi: 10.14440/jbm.2016.122
