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Horse Anti-Mouse IgG Antibody (H+L), DyLight? 649
Description
DyLight 649 Horse Anti-Mouse IgG Antibody can be used?for immunofluorescence?and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.
Features:
- Recognizes both heavy and light chains (H+L)?
- Optimally labeled with DyLight? 649 to provide the brightest label for fluorescence microscopy?
- Supplied in solution?
- Excitation: 655 nm?
- Emission: 670 nm?
- Color: Far Red
Specifications
| Unit Size | 1.5 mg |
|---|---|
| Applications | Immunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation |
| Concentration | 1.5 mg active conjugate/ml |
| Recommended Storage | 2-8 °C |
| Solution | 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. |
| Maximum Emission | 670 nm |
| Maximum Excitation | 665 nm |
| Recommended Usage | Recommended concentration range for use 5-20 μg/ml. If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2% normal serum from the same species as the tissue. |
| Target Species | Mouse |
| Conjugate | DyLight 649 |
| Color of Fluorescence | Far Red |
| Host Species | Horse |
| Format | Concentrate |
Documents
- Safety Data Sheet
- Download CoA
- Datasheet
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Citations
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DyLight??dyes offer a number of potential advantages including greater photostability and brighter fluorescence. ?DyLight??dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight??dyes can be applied as single labels or in combination with other DyLight??dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology. ?The Dylight??dyes currently offered are DyLight??488 (green), DyLight??549 (orange), DyLight??594 (red), and DyLight??649 (far red).
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