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VECTASTAIN? ABC-AmP Reagent (Standard, Western Blot Detection)
Description
The VECTASTAIN?ABC-AmP is an amplified ABC alkaline phosphatase reagent for the detection of mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes. The signal can be visualized using either a chemiluminescent / chemifluorescent or a chromogenic substrate. Used with the DuoLuX Chemiluminescent / Chemifluorescent Substrate, the VECTASTAIN?ABC- AmP system produces a very high and sustained light emission signal, with low background, and permanent fluorescence.
Features:
- Uses biotin/streptavidin technology
- Can detect as little as 1 pg target protein when used with DuoLuX Substrate
- Standard Kit contains Reagent A and Reagent B only
- Sufficient reagents to develop twenty 100 cm2 blots
Specifications
| Unit Size | 1 kit |
|---|---|
| Applications | Blotting Applications, Elispot |
| Technology | ABC (Streptavidin/Biotin) Systems |
| Detection Enzyme(s) | Alkaline Phosphatase |
| Target Species | No antibody included |
| Format | Concentrate |
Kit Components
Kit Contents:
- 0.5 ml Reagent A and 0.5 ml Reagent B
Documents
- User Guide
- Troubleshooting Guide for VECTASTAIN ABC-AmP Kits
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
ImmPACT? Vector? Red Substrate, Alkaline Phosphatase (AP) Catalog #: SK-5105
Vector? Black Substrate Kit, Alkaline Phosphatase (AP) Catalog #: SK-5200
Vector? Blue Substrate Kit, Alkaline Phosphatase (AP) Catalog #: SK-5300
DuoLuX? Chemiluminescent and Fluorescent Substrate, Alkaline Phosphatase (AP) Catalog #: SK-6605-100
10x Casein Solution Catalog #: SP-5020-250
Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated Catalog #: BA-1000-1.5
Horse Anti-Mouse IgG Antibody (H+L), Biotinylated Catalog #: BA-2000-1.5
DuoLuX? Chemiluminescent and Fluorescent Substrate, Peroxidase (HRP) Catalog #: SK-6604-200
BCIP/NBT Substrate Kit, Alkaline Phosphatase (AP), (5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium) Catalog #: SK-5400
Citations
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Greater sensitivity can be achieved by using an amplified detection procedure. Our biotin/avidin and biotin/streptavidin systems introduce a large number of enzymes to the target site providing signal amplification while maintaining low background.