For research use only!

Storage Conditions:?store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life:?12 months after date of delivery

Molecular Formula:?C₁₅H₂₃N₅O₁₆P₂

Molecular Weight:?591.32 g/mol

Exact Mass:?591.06 g/mol

Purity:?≥ 98 % (HPLC)

Form:?solid

Color:?white to off-white

Solubility:?water, aqueous buffer such as PBS

Applications:
Glycosylation of 5-hydroxymethylcytosine (5-hmC) with T4 ?-Glycosyltransferase (T4 ?-GT)^[1-3]
The resulting azide-functionalized, glycosylated 5-hmC moiety can subsequently be detected via Cu(I)-free click chemistry that offers the choice

• to introduce a (Desthio)Biotin group for subsequent purification tasks (via DBCO-containing (Desthio)Biotin)
• to introduce fluorescent group for subsequent microscopic imaging (via DBCO-containing fluorescent dyes)

0.5 mg UDP-6-azide-glucose are sufficient to prepare 283 μl of a 3 mM solution.應(yīng)用：
5-羥甲基胞嘧啶 (5-hmC) 與 T4 ?-糖基轉(zhuǎn)移酶 (T4 ?-GT) 的糖基化 [1-3]
生成的疊氮化物功能化、糖基化的 5-hmC 部分隨后可以通過提供選擇的無 Cu(I) 點擊化學(xué)進行檢測

為后續(xù)純化任務(wù)引入 (Desthio)Biotin 基團（通過含 DBCO 的 (Desthio)Biotin）
為隨后的顯微成像引入熒光基團（通過含 DBCO 的熒光染料）
0.5 mg UDP-6-azide-glucose 足以制備 283 μl 的 3 mM 溶液。

Selected References: 參考文獻
[1] Song?et al.?(2011) Selective chemical labeling reveals the genome-wide distribution of 5-hydroxymethylcytosine.?Nature Biotech?29(1):68.
[2] Li?et al.?(2012) Selective Capture of 5-hydroxymethylcytosine from Genomic DNA.?J. Vis. Exp.?68:e4441.
[3] Song?et al.?(2016) Simultaneous single-molecule epigenetic imaging of DNA methylation and hydroxymethylation.?PNAS?113(16):4339.