離子去污劑
離子除垢劑通常含有陰離子或陽離子頭部基團(tuán)及一個凈電荷。其疏水尾部不是十二烷基硫酸鈉(SDS)和十六烷基三甲基溴化銨(CTAB)等可見的直鏈烴,就是膽汁酸鹽等包含的剛性甾體基團(tuán)。離子除垢劑溶解膜蛋白非常有效,但基本都會導(dǎo)致一定程度的變性。膽汁酸鹽是一種陰離子除垢劑,其主鏈由剛性甾體基團(tuán)組成,例如膽酸鈉鹽和脫氧膽酸鈉鹽等。這類分子的平面結(jié)構(gòu)使其具有一個極性面和一個非極性面,因此,其CMC較高,膠束較小,易于通過透析去除。
非離子去污劑
非離子除垢劑含不帶電的親水頭部基團(tuán),該基團(tuán)由BRIJR和TRITON?等除垢劑中的聚氧乙烯部分或辛基葡糖苷和十二烷基麥芽糖苷等中的糖苷基團(tuán)組成。非離子除垢劑會破壞脂質(zhì)-脂質(zhì)和脂質(zhì)-蛋白互作,但不會破壞蛋白-蛋白的互作,所以是公認(rèn)的非變性除垢劑,因此廣泛應(yīng)用于分離生物活性形式的膜蛋白。另外,與離子除垢劑不同,非離子除垢劑的膠束尺寸受鹽的影響很小。
兩性離子去污劑
兩性離子除垢劑兼具離子和非離子除垢劑的特性。與非離子除垢劑一樣,兩性離子除垢劑不具有凈電荷,缺乏導(dǎo)電性和電泳遷移率,且不與離子交換樹脂結(jié)合。因此是離子交換層析、電泳和等電聚焦中常用的非離子除垢劑的替代物。然而,像離子洗滌劑一樣,它們能有效地打破蛋白質(zhì)與蛋白質(zhì)之間相互作用。另外,甾體類兩性離子除垢劑(如CHAPS)相比直鏈兩性離子除垢劑(如十二烷基二甲基二氧化胺)變性能力通常更弱。
P1379
TWEEN ? 20, viscous liquid
X100
Triton? X-100, laboratory grade
L3771
十二烷基硫酸鈉, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)
T8787
Triton? X-100, for molecular biology
P9416
TWEEN ? 20, for molecular biology, viscous liquid
P1754
TWEEN ? 80, viscous liquid
P7949
TWEEN ? 20, BioXtra, viscous liquid
PVP40
聚乙烯吡咯烷酮, average mol wt 40,000
D6750
脫氧膽酸鈉, ≥97% (titration)
L4509
十二烷基硫酸鈉, ReagentPlus?, ≥98.5% (GC)
436143
十二烷基硫酸鈉, ACS reagent, ≥99.0%
I6758
IPTG, ≥99% (TLC), ≤0.1% Dioxane
T9284
Triton? X-100, BioXtra
P2443
Pluronic? F-127, powder, BioReagent, suitable for cell culture
93443
Triton? X-100 溶液, BioUltra, for molecular biology, ~10% in H2O
H5882
溴化十六烷基三甲銨, ≥98%
71736
十二烷基硫酸鈉 溶液, BioUltra, for molecular biology, 10% in H2O
I8896
IGEPAL? CA-630, for molecular biology
A6426
消泡劑 204, mixture of organic polyether dispersions
O8380
1-辛烷磺酸 鈉鹽, ≥98%
I3021
IGEPAL? CA-630, viscous liquid
H2766
1-庚烷磺酸鈉
H6269
溴化十六烷基三甲銨, for molecular biology, ≥99%
P2287
TWEEN ? 20, viscous liquid, suitable for cell culture
P9769
十乙二醇單十二烷基醚, nonionic surfactant
D141
毛地黃皂苷, Used as non-ionic detergent
75746
十二烷基硫酸鈉, ≥99.0% (GC), dust-free pellets
P4780
TWEEN ? 80, suitable for cell culture, suitable for insect cell culture, viscous liquid
88315
Thesit?, for membrane research
59924
聚山梨酯80, tested according to Ph. Eur.
89510
聚(乙二醇), BioUltra, 8,000
P5556
Poloxamer 188 溶液, 10%, sterile-filtered, BioReagent, suitable for insect cell culture
G4376
L -氧化谷胱甘肽, ≥98% (HPLC)
C5135
Kolliphor? EL, pH range 6.0-8.0
P8136
聚乙烯醇, 87-90% hydrolyzed, average mol wt 30,000-70,000
12060
苯扎氯銨, ≥95.0% (T)
C3023
CHAPS水合物, ≥98% (HPLC)
T7505
四甲基氫氧化銨 五水合物, ≥97%
H26456
二乙二醇, ReagentPlus?, 99%
59920C
EX-CELL?止泡劑, gamma irradiated
71725
十二烷基硫酸鈉, BioUltra, for molecular biology, ≥99.0% (GC)
30970
脫氧膽酸鈉, BioXtra, ≥98.0% (dry matter, NT)
H5269
己烷磺酸鈉, ≥98% (elemental analysis)
K4894
Kolliphor? HS 188, solid
42966
Kolliphor? HS 15
P5413
聚(乙二醇), for molecular biology, average mol wt 8,000
S6760
Span? 80, nonionic surfactant
74882
1-辛烷磺酸鈉 一水合物, suitable for ion pair chromatography, LiChropur?, ≥99.0% (T)
A5757
消泡劑 B 乳液, aqueous-silicone emulsion
B4184
Brij? L23 溶液, 30 % (w/v) in H2O
D8638
十二烷基三甲基溴化銨, ≥98%
40236
十二烷基二甲基氧化胺 溶液, ~30% in H2O
47036
皂素, for molecular biology, used as non-ionic surfactant
X100RS
Triton? X-100(還原型)
P8074
TWEEN ? 80, BioXtra, viscous liquid
L5750
十二烷基硫酸鈉, 92.5-100.5% based on total alkyl sulfate content basis
A8011
消泡劑C乳液, aqueous-silicone emulsion
X114
Triton? X-114, laboratory grade
L6026
十二烷基硫酸鈉, BioXtra, ≥99.0% (GC)
B8879
芐索氯銨, ≥97% (titration), ≥98% (HPLC)
05030
十二烷基硫酸鈉 溶液, BioUltra, for molecular biology, 20% in H2O
T4009
?;悄懰?鈉鹽 水合物, ≥95% (HPLC)
T0875
牛磺脫氧膽酸鈉 水合物, ≥95% (HPLC)
L9150
N -月桂酰肌氨酸 鈉鹽, detergent for use in cell lysis
P3015
聚(乙二醇), average mol wt 200
A8311
消泡劑 204, aqueous emulsion for bacterial and mammalian systems
15S9
TERGITOL烷氧基聚乙烯氫氧基乙醇(非離子表面活性劑)?, Type 15-S-9
03394
聚(乙二醇), tested according to Ph. Eur., 6,000
30326
EMPIGEN? BB去污劑, ~30% active substance
86339
牛膽酸鈉 水合物, ≥97.0% (TLC)
84510
皂素, used as non-ionic surfactant
G7132
甘膽酸鈉 水合物, ≥95% (TLC)
51832
1-庚烷磺酸鈉 一水合物, suitable for ion pair chromatography, LiChropur?, ≥99.0% (T)
52862
1-己烷磺酸鈉 一水合物, suitable for ion pair chromatography, LiChropur?, ≥99.0% (T)
10783641001
聚乙二醇1500, Mr ~1500, pkg of 10 × 4 mL, solution
L5125
N -月桂酰肌氨酸 鈉鹽, ≥94%
H9151
溴化十六烷基三甲銨, BioXtra, ≥99%
15759
Kolliphor? P 188
G0759
甘氨鵝脫氧膽酸鈉
16758
泊洛沙姆407, purified, non-ionic