默克西格瑪去污劑/離子去污劑/非離子去污劑/兩性離子去污劑

離子去污劑

離子除垢劑通常含有陰離子或陽離子頭部基團(tuán)及一個凈電荷。其疏水尾部不是十二烷基硫酸鈉(SDS)和十六烷基三甲基溴化銨(CTAB)等可見的直鏈烴,就是膽汁酸鹽等包含的剛性甾體基團(tuán)。離子除垢劑溶解膜蛋白非常有效,但基本都會導(dǎo)致一定程度的變性。膽汁酸鹽是一種陰離子除垢劑,其主鏈由剛性甾體基團(tuán)組成,例如膽酸鈉鹽和脫氧膽酸鈉鹽等。這類分子的平面結(jié)構(gòu)使其具有一個極性面和一個非極性面,因此,其CMC較高,膠束較小,易于通過透析去除。

非離子去污劑

非離子除垢劑含不帶電的親水頭部基團(tuán),該基團(tuán)由BRIJR和TRITON?等除垢劑中的聚氧乙烯部分或辛基葡糖苷和十二烷基麥芽糖苷等中的糖苷基團(tuán)組成。非離子除垢劑會破壞脂質(zhì)-脂質(zhì)和脂質(zhì)-蛋白互作,但不會破壞蛋白-蛋白的互作,所以是公認(rèn)的非變性除垢劑,因此廣泛應(yīng)用于分離生物活性形式的膜蛋白。另外,與離子除垢劑不同,非離子除垢劑的膠束尺寸受鹽的影響很小。

兩性離子去污劑

兩性離子除垢劑兼具離子和非離子除垢劑的特性。與非離子除垢劑一樣,兩性離子除垢劑不具有凈電荷,缺乏導(dǎo)電性和電泳遷移率,且不與離子交換樹脂結(jié)合。因此是離子交換層析、電泳和等電聚焦中常用的非離子除垢劑的替代物。然而,像離子洗滌劑一樣,它們能有效地打破蛋白質(zhì)與蛋白質(zhì)之間相互作用。另外,甾體類兩性離子除垢劑(如CHAPS)相比直鏈兩性離子除垢劑(如十二烷基二甲基二氧化胺)變性能力通常更弱。

P1379

TWEEN ? 20, viscous liquid

X100

Triton? X-100, laboratory grade

L3771

十二烷基硫酸鈉, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

T8787

Triton? X-100, for molecular biology

P9416

TWEEN ? 20, for molecular biology, viscous liquid

P1754

TWEEN ? 80, viscous liquid

P7949

TWEEN ? 20, BioXtra, viscous liquid

PVP40

聚乙烯吡咯烷酮, average mol wt 40,000

D6750

脫氧膽酸鈉, ≥97% (titration)

L4509

十二烷基硫酸鈉, ReagentPlus?, ≥98.5% (GC)

436143

十二烷基硫酸鈉, ACS reagent, ≥99.0%

I6758

IPTG, ≥99% (TLC), ≤0.1% Dioxane

T9284

Triton? X-100, BioXtra

P2443

Pluronic? F-127, powder, BioReagent, suitable for cell culture

93443

Triton? X-100 溶液, BioUltra, for molecular biology, ~10% in H2O

H5882

溴化十六烷基三甲銨, ≥98%

71736

十二烷基硫酸鈉 溶液, BioUltra, for molecular biology, 10% in H2O

I8896

IGEPAL? CA-630, for molecular biology

A6426

消泡劑 204, mixture of organic polyether dispersions

O8380

1-辛烷磺酸 鈉鹽, ≥98%

I3021

IGEPAL? CA-630, viscous liquid

H2766

1-庚烷磺酸鈉

H6269

溴化十六烷基三甲銨, for molecular biology, ≥99%

P2287

TWEEN ? 20, viscous liquid, suitable for cell culture

P9769

十乙二醇單十二烷基醚, nonionic surfactant

D141

毛地黃皂苷, Used as non-ionic detergent

75746

十二烷基硫酸鈉, ≥99.0% (GC), dust-free pellets

P4780

TWEEN ? 80, suitable for cell culture, suitable for insect cell culture, viscous liquid

88315

Thesit?, for membrane research

59924

聚山梨酯80, tested according to Ph. Eur.

89510

聚(乙二醇), BioUltra, 8,000

P5556

Poloxamer 188 溶液, 10%, sterile-filtered, BioReagent, suitable for insect cell culture

G4376

L -氧化谷胱甘肽, ≥98% (HPLC)

C5135

Kolliphor? EL, pH range 6.0-8.0

P8136

聚乙烯醇, 87-90% hydrolyzed, average mol wt 30,000-70,000

12060

苯扎氯銨, ≥95.0% (T)

C3023

CHAPS水合物, ≥98% (HPLC)

T7505

四甲基氫氧化銨 五水合物, ≥97%

H26456

二乙二醇, ReagentPlus?, 99%

59920C

EX-CELL?止泡劑, gamma irradiated

71725

十二烷基硫酸鈉, BioUltra, for molecular biology, ≥99.0% (GC)

30970

脫氧膽酸鈉, BioXtra, ≥98.0% (dry matter, NT)

H5269

己烷磺酸鈉, ≥98% (elemental analysis)

K4894

Kolliphor? HS 188, solid

42966

Kolliphor? HS 15

P5413

聚(乙二醇), for molecular biology, average mol wt 8,000

S6760

Span? 80, nonionic surfactant

74882

1-辛烷磺酸鈉 一水合物, suitable for ion pair chromatography, LiChropur?, ≥99.0% (T)

A5757

消泡劑 B 乳液, aqueous-silicone emulsion

B4184

Brij? L23 溶液, 30 % (w/v) in H2O

D8638

十二烷基三甲基溴化銨, ≥98%

40236

十二烷基二甲基氧化胺 溶液, ~30% in H2O

47036

皂素, for molecular biology, used as non-ionic surfactant

X100RS

Triton? X-100(還原型)

P8074

TWEEN ? 80, BioXtra, viscous liquid

L5750

十二烷基硫酸鈉, 92.5-100.5% based on total alkyl sulfate content basis

A8011

消泡劑C乳液, aqueous-silicone emulsion

X114

Triton? X-114, laboratory grade

L6026

十二烷基硫酸鈉, BioXtra, ≥99.0% (GC)

B8879

芐索氯銨, ≥97% (titration), ≥98% (HPLC)

05030

十二烷基硫酸鈉 溶液, BioUltra, for molecular biology, 20% in H2O

T4009

?;悄懰?鈉鹽 水合物, ≥95% (HPLC)

T0875

牛磺脫氧膽酸鈉 水合物, ≥95% (HPLC)

L9150

N -月桂酰肌氨酸 鈉鹽, detergent for use in cell lysis

P3015

聚(乙二醇), average mol wt 200

A8311

消泡劑 204, aqueous emulsion for bacterial and mammalian systems

15S9

TERGITOL烷氧基聚乙烯氫氧基乙醇(非離子表面活性劑)?, Type 15-S-9

03394

聚(乙二醇), tested according to Ph. Eur., 6,000

30326

EMPIGEN? BB去污劑, ~30% active substance

86339

牛膽酸鈉 水合物, ≥97.0% (TLC)

84510

皂素, used as non-ionic surfactant

G7132

甘膽酸鈉 水合物, ≥95% (TLC)

51832

1-庚烷磺酸鈉 一水合物, suitable for ion pair chromatography, LiChropur?, ≥99.0% (T)

52862

1-己烷磺酸鈉 一水合物, suitable for ion pair chromatography, LiChropur?, ≥99.0% (T)

10783641001

聚乙二醇1500, Mr ~1500, pkg of 10 × 4 mL, solution

L5125

N -月桂酰肌氨酸 鈉鹽, ≥94%

H9151

溴化十六烷基三甲銨, BioXtra, ≥99%

15759

Kolliphor? P 188

G0759

甘氨鵝脫氧膽酸鈉

16758

泊洛沙姆407, purified, non-ionic