德國MN NucleoBond Xtra Midi 超快速轉染級質粒中提試劑盒 740410.50
NucleoBond(R) Xtra Midi / Maxi
德國MN NucleoBond Xtra Midi 超快速轉染級質粒中提試劑盒
貨號：740410.50
規(guī)格：50 preps
品牌：德國MN
從大腸桿菌中提取高拷貝/低拷貝質粒。新一代陰離子交換柱-短時間，高產量，轉染用質粒。
特點：
節(jié)約60%的時間，增加100%的產量
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新一代陰離子交換–超快，高產量的轉染級別的質粒DNA
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優(yōu)化設計的柱子：制備時間約為30 分鐘(Midi prep)；35 分鐘(Maxi prep)
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包含柱過濾器：高過慮流速，清除裂解液中不溶物的同時可向柱子加樣
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改進的硅材料：標準的產量250μg-1000μg
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專li的陰離子交換技術得到轉染級別的質粒DNA
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NucleoBond(R)Xtra Plus kits 包含NucleoBond(R) Finalizers；加速了DNA的沉淀
2nd generation of anion exchanger for fast purification of plasmid DNA
| Selling unit | 10 Prep(s), 50 Prep(s), 100 Prep(s) |
| Application | Transfection-grade plasmid DNA isolation |
| Target | Plasmid DNA |
| CE certified | No, research use only |
| Technology | Anion exchange chromatography |
| Brand | NucleoBond |
| Format | Midi prep |
| Handling | Gravity flow |
| Lysate clarification | Column filter |
| Automated use | Yes, can be automated on the Andrew+ the pipetting robot |
| Sample material | Bacteria, E. coli |
| Sample amount | < 200 mL (high copy), < 400 mL (low copy) |
| Vector size | < 300 kbp |
| Typical yield | 500 μg |
| Theoretical binding capacity | 800 μg |
| Typical purity A260/A280 | 1.8?1.95 |
| Endotoxin level | 1?10 EU/μg DNA |
| Isopropanol precipitation | Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Midi, NucleoSpin Finisher Midi |
| Preparation time | 70 min/prep |
| Typical downstream application | Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells |
| Storage temperature | 15?25 °C |
| Shelf life (from production) | 39 Month(s) |
| Hazardous material | Yes |
產品概覽
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NucleoBond(R) XtraMidi |
NucleoBond(R) Xtra Maxi |
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技術 |
陰離子交換色譜法 |
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產品形式 |
Midi gravity-flow columns |
Maxi gravity-flow columns |
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裂解液澄清 |
柱過濾器 |
柱過濾器 |
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樣品材料 |
< 200 mL (高拷貝) < 400 mL (低拷貝) |
< 600 mL (高拷貝) < 1 200 mL (低拷貝) |
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載體大小 |
< 300 kbp |
< 300 kbp |
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產量 |
250 μg |
1000 μg |
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A260/A280 |
1.80 – 1.95 |
1.80 – 1.95 |
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異丙醇沉淀 |
Xtra Midi |
Xtra Midi Plus |
XtraMaxi |
XtraMaxi Plus |
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Centrifugation |
NucleoBond(R) Finalizer |
Centrifugation |
NucleoBond(R)Finalizer Large |
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制備時間 |
70min/4preps |
30min/4preps |
80min/4preps |
35min/4preps |