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CircLigase II ssDNA Ligase

An ATP-independent, non-catalytic thermostable ligase that catalyses the intramolecular ligation (i.e. circularisation) of ssDNA and ssRNA templates.

Product Details

CircLigase II ssDNA Ligase is provided at 100 U/μl.

Contents: CircLigase II ssDNA Ligase, CircLigase II 10X Reaction Buffer, 50 mM MnCl2, CircLigase ssDNA Control Oligo, Betaine, Sterile Water.

Key features

  • Intramolecular ligation of single-stranded DNA and RNA ends containing 5′-phosphates and 3′-hydroxyl groups
  • ATP-independent, non-catalytic enzyme
  • Circularises ssDNA 15 bases long
  • Compatible with high temperature ligation reactions due to thermostability of CircLigase II enzyme

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