日本 JaICA代理 Anti HEL monoclonal antibody
日本 JaICA 老化 Antibody系列產(chǎn)品
上海金畔生物作為日本 JaICA 老化中國代理商，歡迎新老客戶訪問日本 JaICA 老化官網(wǎng)或者咨詢我們獲取更多日本 JaICA 老化產(chǎn)品線價格說明書等信息。
日本老化制御研究所?Jaica代理
抗己酰-賴氨酸單克隆抗體 Anti Hexanoyl-Lysine(HEL) monoclonal antibody(5F12)
抗己酰-賴氨酸單克隆抗體 Anti Hexanoyl-Lysine(HEL) monoclonal antibody(5F12)
適用于免疫組織化學和ELISA。
Suitable for immunohistocheistry and ELISA. For research use only.
Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. This monoclonal antibody (clone 5H4)is spesific for proteins and peptides containing HEL. Suitable for HEL detection by immunohistochemistry and western blotting.
由活性氧（ROS）引起的脂質(zhì)氧化損傷在一些疾病，細胞功能損害和衰老中起重要作用。醛類（如丙二醛/MDA）和4-羥基-2-壬烯醛（4-HNE）已被報道為先進的脂質(zhì)過氧化產(chǎn)物之一，但最近在脂質(zhì)過氧化的早期階段，發(fā)現(xiàn)13-hydroperoxyoctadecanoic acid(13-HPODE)與蛋白質(zhì)1)共價結合。
己酰-賴氨酸加合物（HEL）是一種新的脂質(zhì)氫過氧化物修飾的賴氨酸殘基， HEL通過氧化的omega-6脂肪酸（例如亞油酸或花生四烯酸）氧化修飾形成。
抗己酰-賴氨酸（HEL）單克隆抗體對于含有HEL的蛋白質(zhì)和肽是特異的，適用于通過免疫組織化學和免疫印跡的HEL檢測。
A new biomarker for lipid peroxidation? ?脂質(zhì)過氧化新的生物標志物
Cisplatin-treated rat kidney.?順鉑處理的大鼠腎臟
(Dr. Sugiyama, Tottori Univ.)??（Sugiyama博士）
Detection of HEL at Human atherosclerotic lesions.
(Dr. Naito)
HEL在人類動脈粥樣硬化病變的檢測??（Naito博士）
| Clone #: | 5F12 |
| Subclass: | IgG1 kappa |
| Antigen: | N epsilon?hexanoyl-Keyhole limpet hemocyanin (HEL-KLH) |
| Specificity: | Specific to Hexanoyl-Lys adducts. |
| Form: | Lyophilized power. Containing 10mM PBS(pH7.4), 5% sucrose, 1% BSA and 0.05% Procline 950. 100 碌g/mL of IgG. |
| Application: | Immunohistochemistry (Recommended antibody concentration is 2 碌g/mL on paraformaldehyde fixed tissue), and ELISA |
| Storage: | Less than -20C |
? 技術參數(shù)
克?。＃?F12；
亞基：IgG1 kappa；
抗原：N epsilon己酰-鑰孔血藍蛋白（HEL-KLH）；
特異性：特異于己酰-賴氨酸加合物；
形式：凍干粉，100μg/mL（200μL），PBS（pH7.4），包含5％蔗糖，1％ BSA；
應用：免疫組織化學（多聚甲醛固定的組織推薦抗體濃度為2μg/mL）和ELISA；
儲存：低于-20°C
參考文獻：
| 1) | Yoji Kato, Yoko Mori, Yuko Makino, Yasujiro Morimitsu, Sadayuki Hiroi, Toshitsugu Ishikawa, Toshihiko Osawa, Formation of N epsilon-(hexanonyl) lysine in protein exposed to lipid hydroperoxide. J Biol Chem 274(29), p20406-20414 (1999) Identification of HEL, which is lysine adduct of 13-HPODE. |
| 2) | Kato Y, Miyake Y, Yamamoto K, Shimomura Y, Ochi H, Mori Y, Osawa T, Preparation of a monoclonal antibody to N(epsilon)-(Hexanonyl)lysine: application to the evaluation of protective effects of flavonoid supplementation against exercise-induced oxidative stress in rat skeletal muscle. Biochem Biophys Res Commun 274(2),p389-393(2000) Development and characterization of anti HEL monoclonal antibody. |
| 3) | Fukuchi Y, Miura Y, Nabeno Y, Kato Y, Osawa T, Naito M, Immunohistochemical detection of oxidative stress biomarkers, dityrosine and N(epsilon)-(hexanoyl)lysine, and C-reactive protein in rabbit atherosclerotic lesions. J Atheroscler Thromb 15(4)p185-192(2008) Application to rabbit atherosclerotic lesions. |
| 4) | Maeda R, Noiri E, Isobe H, Homma T, Tanaka T, Negishi K, Doi K, Fujita T, Nakamura E, A water-soluble fullerene vesicle alleviates angiotensin II-induced oxidative stress in human umbilical venous endothelial cells. Hypertens Res 31(1)p141-151(2008) Application to cultured cells (HUVECs). |
| 5) | Sango K, Yanagisawa H, Kato K, Kato N, Hirooka H, Watabe K: Differential Effects of High Glucose and Methylglyoxal on Viability and Polyol Metabolism in Immortalized Adult Mouse Schwann Cells. Open Diabetes J. 1, p1-11(2008) Application to cultured cell from mouse. |
產(chǎn)品信息：
| Product name | Code | Specifications | Application | NOTICE |
| Anti HEL monoclonal antibody(clone 5F12) | MHL-021P | 20ug of IgG | Immunohistochemistry | Current product |
| Anti HEL monoclonal antibody(clone 5H4) | MHL-020P | 20ug of IgG | Immunohistochemistry | Discontinued |
使用說明書
免疫組織化學方法
技術信息
克隆#5F12和5H4的比較
| Instruction manual (PDF) | Immunohistochemical procedure | Technical information |
| Comparison of clone 5F12 and 5H4 (PDF) | ||