如何发布网络小说,梦入神机

日本JaICA 代理Hexanoyl-Lysine adduct(HEL) ELISA Kit

Hexanoyl-lysine adduct (HEL) ELISA kit

Suitable for assessment of oxidative stress using urine, serum and cultured cells. For research use only.

已酰-賴氨酸（HEL）ELISA試劑盒，適用于尿液，血清和細(xì)胞培養(yǎng)樣品氧化應(yīng)激的評(píng)估。
僅供研究使用。

什么是己酰-賴氨酸？

Biomarker for early stage of lipid oxidation

Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. HEL may be a useful biomarker for initial stage of lipid peroxidation.Monoclonal antibodies and ELISA kit have been developped, and HEL can be detected in oxidatively modified LDL, in human atherosclerotic lesions, human urine and serum. It is also reported that HEL is formed in rat muscle during exercise, and the formation is prohibited by antioxidants such as flavonoids.

由活性氧（ROS）引起的脂質(zhì)氧化損傷在一些疾病、細(xì)胞功能損害和衰老中起重要作用。醛類如丙二醛（MDA）和4-羥基-2-壬烯醛（4-HNE）已被報(bào)道為先進(jìn)的脂質(zhì)過(guò)氧化產(chǎn)物之一。但最近在脂質(zhì)過(guò)氧化的早期階段，發(fā)現(xiàn)13-氫過(guò)氧十八烷酸（13-HPODE）與蛋白質(zhì)共價(jià)結(jié)合。己酰-賴氨酸加合物（HEL）是一種新的脂質(zhì)氫過(guò)氧化物修飾的賴氨酸殘基。HEL是通過(guò)氧化的omega-6脂肪酸（例如亞油酸或花生四烯酸）的氧化修飾形成。HEL可能是脂質(zhì)過(guò)氧化初始階段有用的生物標(biāo)志物。現(xiàn)在我們已經(jīng)開發(fā)了HEL單克隆抗體和ELISA試劑盒，并且可以在氧化修飾的低密度脂蛋白（LDL）、人動(dòng)脈粥樣硬化病變、人尿液和血清中檢測(cè)HEL。另?yè)?jù)報(bào)道，大鼠肌肉在運(yùn)動(dòng)期間形成HEL，并且通過(guò)抗氧化劑（如類黃酮）是禁止形成的。

己酰-賴氨酸（HEL）ELISA試劑盒

JaICA have developed HEL ELISA kit in collaboration with Dr. Toshihiko Osawa (Nagoya University) and Dr. Yoji Kato (Univerisity of Hyogo). This ELISA kit can be applied to urine, serum and cultured cells form human and animal.

JaICA公司與Toshihiko Osawa博士（名古屋大學(xué)）和Yoji Kato博士（兵庫(kù)大學(xué)）合作開發(fā)了Hexanoyl-Lys（HEL）ELISA試劑盒。
該ELISA試劑盒可應(yīng)用于人和動(dòng)物的尿液、血清和細(xì)胞培養(yǎng)。

Assay principle:	Competitive ELISA (detection: 450 nm)
Specifity:	Specific to N-epsilon-Hexanoyl-Lysine adduct
Measuring range:	2 – 700 nmol/L
Time to answer:	Over night and 2 hours.
Format:	96 wells (54 samples in single assay)
Applications:	Urine, serum and cultured cells from human and animals.
Storage:	Store at 2 – 8擄C (don’t freeze).
Expiry:	2 years after the day of manufacturing.
Required but not provided:	50 碌L micropipettor and pipette tips 8-channel (50-200 碌L) micropipettor and tips 8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet. 4-7 擄C incubator Microtiter plate reader (measuring wavelength 450 nm)

技術(shù)參數(shù)
檢測(cè)原理：競(jìng)爭(zhēng)法ELISA (檢測(cè): 450 nm處)；
特異性：特異于N-epsilon-己酰-賴氨酸加合物；
測(cè)定范圍：2 – 700 nmol/L；
反應(yīng)速度：過(guò)夜+2小時(shí)；
規(guī)格：96孔(54 samples in single assay)；
應(yīng)用：人和動(dòng)物的尿液、血清和細(xì)胞培養(yǎng)物；
儲(chǔ)存：2-8℃（避免冷凍）；
保質(zhì)期：2年。

Content of this kit

HEL-coated Microtiter Plate:	1 plate (96 wells)
Primary Antibody (ready to use):	1 vial
Secondary Antibody:	1 vial
Secondary Antibody Buffer:	1 vial
Chromogen (TMBZ solution):	1 vial
Chromogen Buffer:	1 vial
Washing Buffer (5X):	1 vial
Stop Solution:	1 vial
Standard solution (6 levels):	1 vial each
Plate seal:	2 sheets

試劑盒組成：

HEL-包被微孔板: 1 塊板(96孔)
一抗 (即用型): 1?瓶
二抗: 1?瓶
二抗緩沖液: 1?瓶
底物溶液 (TMBZ溶液): 1?瓶
底物緩沖液: 1 瓶
洗滌液 (5X): 1 瓶
終止液: 1 瓶
標(biāo)準(zhǔn)液 (6 標(biāo)準(zhǔn)): 各1 瓶
封板膜: 2 片

Product name	Code	Assay range	Application
Hexanoyl-Lys (HEL) ELISA	KHL-700E	2-700 nmol/L	Urine, serum, cultured cells and other biological materials

參考文獻(xiàn)：

1)
Yoji Kato, Yoko Mori, Yuko Makino, Yasujiro Morimitsu, Sadayuki Hiroi, Toshitsugu Ishikawa, Toshihiko Osawa, Formation of N epsilon-(hexanonyl) lysine in protein exposed to lipid hydroperoxide. J Biol Chem 274(29), p20406-20414 (1999)
Identification of HEL, which is lysine adduct of 13-HPODE.
2)
Yoji Kato, Toshihiko Osawa: Detection of lipid hydroperoxide-derived protein modification with polyclonal antibodies. Methods in Molecular Biology, vol. 186, Oxidative stress biomarkers and antioxidant protocols, D Armstrong, Ed., Human Press Inc., NJ, USA, p37-44
Immunohistochemical detection of hexanoyl-lysine adduct.
3)
Kato Y, Miyake Y, Yamamoto K, Shimomura Y, Ochi H, Mori Y, Osawa T, Preparation of a monoclonal antibody to N(epsilon)-(Hexanonyl)lysine: application to the evaluation of protective effects of flavonoid supplementation against exercise-induced oxidative stress in rat skeletal muscle. Biochem Biophys Res Commun 274(2),p389-393(2000)
Development and characterization of anti HEL monoclonal antibody.
4)
Ryo K, Yamada H, Nakagawa Y, Tai Y, Obara K, Inoue H, Mishima K, Saito I, Possible involvement of oxidative stress in salivary gland of patients with Sjogren’s syndrome. Pathobiology 73(5), p252-260 (2006)
HEL can be detected in saliva samples from patients with Sjogren’s syndrome.
5)
Suzuki T, Kazui T, Yamamoto S, Washiyama N, Ohkura K, Ohishi K, Bashar AH, Yamashita K, Terada H, Suzuki K, Akuzawa S, Fujie M, Effect of prophylactically administered edaravone during antegrade cerebral perfusion in a canine model of old cerebral infarction. J Thorac Cardiovasc Surg 133(3),p710-716 (2007)
Application to canine serum samples.
6)
Shimizu K, Ogawa F, Akiyama Y, Muroi E, Yoshizaki A, Iwata Y, Komura K, Bae S, Sato S, Increased Serum Levels of N(epsilon)-(hexanoyl)lysine, A New Marker of Oxidative Stress, in Systemic Sclerosis. J Rheumatol. 2008 Sep 1.
Serum HEL concentration from systemic sclerosis is higher than that from healthy subjects.
7)
Tamura H, Takasaki A, Miwa I, Taniguchi K, Maekawa R, Asada H, Taketani T, Matsuoka A, Yamagata Y, Shimamura K, Morioka H, Ishikawa H, Reiter RJ, Sugino N, Oxidative stress impairs oocyte quality and melatonin protects oocytes from free radical damage and improves fertilization rate. J Pineal Res 44(3),p280-287(2008)
Intrafollicular concentration of HEL was significantly reduced by these antioxidant treatment.
8)
Sakamoto Y, Ishikawa T, Kondo Y, Yamaguchi K, Fujisawa M, The assessment of oxidative stress in infertile patients with varicocele. BJU Int 101(12), p1547-1552 (2008)
Azoospermic and oligospermic patients had a significantly higher HEL concentration in seminal plasma.
9)
Kageyama Y, Takahashi M, Nagafusa T, Torikai E, Nagano A, Etanercept reduces the oxidative stress marker levels in patients with rheumatoid arthritis. Rheumatol Int 28(3),pp245-251(2008)
Urinary HEL level was reduced at 3 and 6 months after the initial treatment with etanercept.
10)
Rummenie VT, Matsumoto Y, Dogru M, Wang Y, Hu Y, Ward SK, Igarashi A, Wakamatsu T, Ibrahim O, Goto E, Luyten G, Inoue H, Saito I, Shimazaki J, Tsubota K, Tear cytokine and ocular surface alterations following brief passive cigarette smoke exposure. Cytokine 43(2),p200-208(2008)
HEL concentration in tear was increased by brief passive exposure to cigarette smoke.
11)
K Sakai, S Kino, A Masuda, M Takeuchi, T Ochi, J Osredkar, B Rejc, K Gersak, N Ramarathnam, Y Kato;
Determination of HEL (Hexanoyl-Lysine Adduct): A Novel Biomarker for Omega-6 PUFA Oxidation.
Lipid Hydroperoxide-Derived Modification of Biomolecules: Subcellular Biochemistry 77,p61-72(2014)
An original paper for HEL ELISA development.