A systematic screen spanning the key values of pH, precipitant type / concentration and salts for alpha-type transmembrane proteins using vapour diffusion methods. 48 conditions allowing the pH range, precipitants and salts used in membrane protein crystallization to be screened with detergent containing protein drops. The reagents can be easily arranged in systematic array to facilitate the interpretation of results and the design of further optimization experiments.

Features of MemSys™:

• A systematic approach to screening for initial crystallization conditions for membrane proteins using vapour diffusion methods.
• Membrane protein solubility is pushed to the limit to provide more information than previous sparse matrix type screens.
• Includes the pH, precipitant concentration and type, and salts found to be successful.
• Primarily designed for alpha-type transmembrane proteins, but has also been successfully applied to beta-type outer membrane proteins.

Developed by members of the So Iwata Lab, Imperial College, London. This product is manufactured under an exclusive licence from Imperial College of Science, Technology & Medicine, London, UK.

使用蒸汽擴散方法對 α 型跨膜蛋白的 pH 值、沉淀劑類型/濃度和鹽的關鍵值進行系統(tǒng)篩選。 48 種條件允許使用含有蛋白質(zhì)滴劑的洗滌劑篩選膜蛋白質(zhì)結晶中使用的 pH 值范圍、沉淀劑和鹽。試劑可以很容易地排列在系統(tǒng)陣列中，以方便結果的解釋和進一步優(yōu)化實驗的設計。

MemSys™ 的特點：

使用蒸汽擴散方法篩選膜蛋白初始結晶條件的系統(tǒng)方法。
膜蛋白溶解度被推到極限，以提供比以前稀疏矩陣類型屏幕更多的信息。
包括 pH 值、沉淀劑濃度和類型以及成功的鹽類。
主要設計用于 α 型跨膜蛋白，但也已成功應用于 β 型外膜蛋白。
由倫敦帝國理工學院 So Iwata 實驗室的成員開發(fā)。本產(chǎn)品在英國倫敦帝國理工學院的獨家許可下生產(chǎn)。

References? ?參考文獻
“Methods and Results in the crystallization of Membrane proteins” (2003) Ed. Iwata S. (International University Line)