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Solanum Tuberosum (Potato) Lectin (STL, PL), Biotinylated
Description
This lectin binds oligomers of?N-acetylglucosamine and some bacterial cell wall oligosaccharides containing?N-acetylglucosamine and?N-acetylmuramic acid. Although the carbohydrate binding specificity is similar to wheat germ agglutinin and?Datura stramonium?lectin, several differences have been reported for potato lectin.
Biotinylated?Solanum tuberosum?lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
Specifications
| Unit Size | 2 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology |
| Recommended Usage | For most applications we recommend a freshly prepared working solution of 5-20 μg/ml in the above buffer. |
| Recommended Storage | 2-8 °C; Store frozen for long term storage |
| Solution | 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2, 20 mM N-acetylglucosamine. |
| Concentration | 2 mg active conjugate/ml |
| Conjugate | Biotinylated |
| Sugar Specificity | N-Acetylglucosamine |
Documents
- Lectins in Histochemistry, ELISA, and Western Blot Applications
- Safety Data Sheet
- Download CoA
- Datasheet
Product FAQs
I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?
Related Products
Chitin Hydrolysate Catalog #: SP-0090-10
Solanum Tuberosum (Potato) Lectin (STL, PL), Unconjugated Catalog #: L-1160-5
Lectin Kit III, Biotinylated Catalog #: BK-3000
Citations
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This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.
Inhibiting/Eluting Sugar: Chitin Hydrolysate