Vector Labs代理，歡迎訪問Vector Labs官網(wǎng)或者咨詢我們獲取相關(guān)Vector Labs產(chǎn)品信息以及報(bào)價(jià)。
Vector Labs 公司是世界著名的生物學(xué)檢測試劑公司，七十年代其在全球首推的生物素-親和素系統(tǒng)引領(lǐng)了生物學(xué)檢測方法的重大革命，該系統(tǒng)被普遍視為目前最靈敏、最可靠與最有效的染色系統(tǒng)，并被廣泛應(yīng)用于免疫組織化學(xué)、免疫電鏡、原位雜交與凝集素化學(xué)中。該系統(tǒng)仍在不斷地發(fā)展，以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產(chǎn)品的主要供應(yīng)商，并提供大量的新產(chǎn)品，如酶底物、神經(jīng)元示蹤劑及蛋白質(zhì)、糖類與核酸的標(biāo)記、分離與檢測試劑。
Sambucus Nigra Lectin (SNA, EBL), Biotinylated
Description
Sambucus nigra?lectin, isolated from elderberry bark,?binds preferentially to sialic acid attached to terminal galactose in α-2,6 and to a lesser degree, α-2,3 linkage. Binding is also inhibited to some extent by lactose or galactose. This lectin appears to bind sialic acid linked to?N-acetylgalactosamine or galactose. SNA has been reported to inhibit cell-free protein synthesis.
Biotinylated?Sambucus nigra?lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
Specifications
| Unit Size | 2 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology |
| Recommended Usage | For most applications, we recommend a freshly prepared working solution of 10 μg/ml in the above buffer. A precipitate may form during storage.This does not have a significant adverse effect on the product.If a precipitate forms upon long-term storage, warm to 37 oC and centrifuge before use. |
| Recommended Storage | 2-8 °C; Store frozen for long term storage |
| Solution | 10 mM HEPES, 015 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2. |
| Concentration | 2 mg active conjugate/ml |
| Conjugate | Biotinylated |
| Sugar Specificity | Sialic Acid |
Documents
- Lectins in Histochemistry, ELISA, and Western Blot Applications
- Safety Data Sheet
- Download CoA
- Datasheet
Product FAQs
I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?
Related Products
Sambucus Nigra Lectin (SNA, EBL), Agarose bound Catalog #: AL-1303-2
Glycoprotein Eluting Solution for Galactose/GalNAc Binding Lectins Catalog #: ES-2100-100
Sambucus Nigra Lectin (SNA, EBL), Fluorescein Catalog #: FL-1301-2
Sambucus Nigra Lectin (SNA, EBL), Unconjugated Catalog #: L-1300-5
Galactose Catalog #: S-9003
Lactose Catalog #: S-9004
Citations
Powered by Bioz See more details on BiozTechnical Information
Elution: 500 mM lactose in buffered saline followed by 500 mM lactose in acetic acid