屬性
生物來源
mouse

質(zhì)量水平
200

偶聯(lián)物
peroxidase conjugate

抗體形式
purified from hybridoma cell culture

antibody product type
primary antibodies

克隆
GG-6, monoclonal

形式
lyophilized powder

species reactivity
human

濃度
~2 mg/mL

technique(s)
direct ELISA: 1:16,000-1:32,000 using 2.5 μg/ml human IgG for coating.

同位素/亞型
IgG1

運輸
dry ice

儲存溫度
20°C

target post-translational modification

unmodified

說明
一般描述
Anti-Human IgG (Fab specific)-Peroxidase antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the GG-6 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with purified human IgG (Fab fragment). IgGs are the most common Immunoglobulins isotype in blood, lymph fluid, cerebrospinal fluid and peritoneal fluid and a key players in the humoral immune response. IgGs include four subclasses (IgG1, IgG2, IgG3, and IgG4), they consist of a variable Fab fragment (which includes the antibody recognition site), and a conserved Fc fragment. The IgG subclasses differ in their physical and chemical properties, their distribution pattern is found to be age-dependent, and every subclass has a specific biological function.
Anti-Human IgG (Fab specific)-Peroxidase antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the GG-6 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with purified human IgG.
免疫原
Purified human IgG (Fab Fragment)
應(yīng)用
Direct ELISA: a working dilution of 1:16,000-1:32,000 is recommended using 2.5 μg/mL human IgG for coating.
生化/生理作用
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. Maternal IgG is the only antibody transported across the placenta to the fetus. Maternal IgG passively immunizes the infants. IgG deficiencies are often associated with various diseases.
外形
Supplied as a lyophilized powder.
其他說明
In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.