Wako 019-19741 Anti Iba1, Rabbit

發(fā)表于由

Wako 和光純藥 Anti Iba1, Rabbit (for Immunocytochemistry)

Iba1抗體，兔源（免疫細(xì)胞化學(xué)用）

品牌 產(chǎn)品編號 產(chǎn)品名稱 包裝
WAKO 019-19741 Anti Iba1, Rabbit (for Immunocytochemistry) 50ug

鈣離子作為信號轉(zhuǎn)導(dǎo)通道中的第一信使，在所有細(xì)胞包括中樞神經(jīng)細(xì)胞的生命活動中起重要作用。鈣離子與不同的鈣結(jié)合蛋白結(jié)合后進(jìn)行級聯(lián)反應(yīng)。Iba1是能與巨噬細(xì)胞和小膠質(zhì)細(xì)胞發(fā)生特異性結(jié)合的鈣結(jié)合蛋白，當(dāng)這些細(xì)胞激活后，Iba1的表達(dá)量上調(diào)，因此Iba1通常作為鑒定小膠質(zhì)細(xì)胞的標(biāo)記物使用。近來研究發(fā)現(xiàn)，小膠質(zhì)細(xì)胞除了提供營養(yǎng)、保護(hù)神經(jīng)的作用外，還被證明可產(chǎn)生NO、TNF-a、IL-1β等物質(zhì)，因此將小膠質(zhì)細(xì)胞定義為中樞神經(jīng)系統(tǒng)的巨噬細(xì)胞樣吞噬細(xì)胞，具有重要的免疫細(xì)胞作用。當(dāng)病原菌、病毒入侵中樞神經(jīng)系統(tǒng)時會起免疫監(jiān)御的作用。近來研究發(fā)現(xiàn)，該細(xì)胞可能與神經(jīng)退行性疾病的發(fā)生和發(fā)展有關(guān)。本產(chǎn)品是可與小膠質(zhì)細(xì)胞發(fā)生特異性反應(yīng)的兔多抗，配合對星形膠質(zhì)細(xì)胞有特異性的GFAP單抗，可進(jìn)行雙染色。

抗原：合成肽（Iba1?的C末端序列）

外觀：TBS溶液（1mg/ml）

提純：抗原親和提純

特異性：對小膠質(zhì)細(xì)胞、巨噬細(xì)胞特異反應(yīng)。不會與神經(jīng)元、星形膠質(zhì)細(xì)胞發(fā)生交叉反應(yīng)。能與人、小鼠、大鼠Iba1反應(yīng)。

用法：IBA1抗體，兔源（免疫細(xì)胞化學(xué)用）適合免疫細(xì)胞化學(xué)。只需1~2μg/ml即可進(jìn)行實驗。

  • Anti?Iba1,?Rabbit

    019-19741(For?Immunocytochemistry)?&?016-20001?(for?Western?Blotting)

    Iba1:?ionized?calcium?binding?adapter?molecule?1?小膠質(zhì)細(xì)胞/巨噬細(xì)胞特異性蛋白抗體

    鈣離子是已知的所有細(xì)胞，包括中樞神經(jīng)系統(tǒng)（CNS）細(xì)胞中最重要的信號調(diào)解人之一。鈣離子通過與各種鈣結(jié)合蛋白結(jié)合發(fā)揮信號活動，其中許多鈣結(jié)合蛋白被劃分成一個大的蛋白家族，EF手性蛋白家族。Iba1是一個17?kDa的EF手性蛋白，在巨噬細(xì)胞和小膠質(zhì)細(xì)胞中表達(dá)，并在這些細(xì)胞的活化過程中表達(dá)升高。
    本抗體以合成的的Iba1羧基末端序列為抗原，該序列是人，大鼠和小鼠Iba1蛋白質(zhì)保守序列。本抗體（免疫組化用）特異性與小膠質(zhì)細(xì)胞/巨噬細(xì)胞反應(yīng)，免疫雙重染色與單克隆抗體結(jié)合，可與GFAP單克隆抗體（(膠質(zhì)纖維酸性蛋白，Glial?Fibrillary?Acidic?Protein）聯(lián)合使用對腦組織和細(xì)胞培養(yǎng)物進(jìn)行雙重染色。

    特點

    S特異性檢測小膠質(zhì)細(xì)胞與/巨噬細(xì)胞，不與神經(jīng)細(xì)胞與星形膠質(zhì)細(xì)胞發(fā)生交叉反應(yīng)。可與人，大鼠，小鼠的Iba1反應(yīng)

    貨號 品名 包裝 用途 工作濃度
    019-19741 Anti?Iba1?polyclonal?antibody,?Rabbit,?for?Immunocytochemistry 50ug(100ul) IH 1-2ug/ml
    016-20001 Anti?Iba1?polyclonal?antibody,?Rabbit,?for?Western?Blotting 50ug(100ul) WB 0.5-1ug/ml

    應(yīng)用

    石蠟切片應(yīng)用方法：
    1.?固定
    2.?用0.01M?PBS緩沖液清洗二次
    3.?使用含有0.3%?過氧化氫的甲醇孵育30分鐘
    4.?用0.01M?PBS緩沖液清洗三次
    5.?使用封閉液（含有1.5%的山羊血清和1%BSA的0.001M?PBS緩沖液）室溫孵育2小時
    6.?使用含有Anti?Iba1?polyclonal?antibody的封閉液4℃孵育過夜
    7.?用0.01M?PBS緩沖液清洗三次
    8.?使用含有生物素標(biāo)記的抗兔IgG（1：200）的封閉液室溫孵育1小時
    9.?用0.01M?PBS緩沖液清洗三次
    10.?使用Elite?ABC試劑（含有試劑A1：50，試劑B1：50的0.01M?PBS溶液）
    11.?用0.01M?PBS緩沖液清洗三次

    12.?過氧化物酶溶液（含有0.01%?過氧化氫和0.05%?DAB的0.05M?Tris溶液）

    英文參考：

    Summary

    This product is for research use only. Do not administer it to human.

    Antibodies against Macrophage/Microglia-specific Protein Iba1

    Calcium ions are known to be one of the most important signal mediators in all cells including central nervous system (CNS) cells. Calcium ions exert their signaling activity through association with various calcium binding proteins, many of which are classified into a large protein family, the EF hand protein family. Iba1 is a 17-kDa EF hand protein that is specifically expressed in macrophages/ microglia and is upregulated during the activation of these cells.Wako has launched rabbit polyclonal antibodies were raised against a synthetic peptide corresponding to the Iba1 carboxy-terminal sequence, which was conserved among human, rat and mouse Iba1 protein sequences. These antibodies are specifically reactive to microglia/ macrophages, are appropriate for immuno-double staining of brain tissues and cell culture in combination with monoclonal antibody to GFAP, which specifically reacts to astrocyte.

    Rabbit Anti Iba1 antibody is raised a synthetic peptide corresponding to C-terminus of Iba1.

    Preparation: Purified by the antigen affinity chromatography from rabbit antisera and prepared in TBS solution. Contains no preservatives and stabilizers.

    Specificity: Specific to microglia and macrophage, but not cross-reactive with neuron and astrocyte. Reactive with human, mouse and rat Iba1.

    Working Concentration: Immunocytochemistry 1-2 micro-g/mL

    Storage:Store at -20 degrees C. After opening, aliquot contents and freeze at -20 degrees C.

    Package Size: 50 ug (100 uL) (ug: micro-g; uL: micro-L)

    References:

    Imai, Y., Ibata,?I., Ito, D., Ohsawa, K. and Kohsaka, S.: Biochem. Biophys. Res. Commun., 224, 855 (1996).

    Ito, D., Imai, Y., Ohsawa, K, Nakajima, K, Fukuuchi, Y. and Kohsaka, S.: Brain Res. Mol. Brain Res., 57, 1 (1998).

    Ohsawa, K., Imai, Y.,?Kanazawa, H., Sasaki, Y. and Kohsaka, S.: J. Cell Sci., 113, 3073 (2000).

    Sasaki, Y., Ohsawa, K.,?Kanazawa, H., Kohsaka, S. and Imai, Y.: Biochem. Biophys. Res. Commun., 286, 292 (2001).

    Kanazawa, H., Ohsawa, K., Sasaki, Y., Kohsaka, S. and Imai, Y.: J. Biol. Chem., 277, 20026(2002).

    Wako catalog #016-20001 Anti Iba1 polyclonal antibody, Rabbit, for Western Blotting (50 micrograms (100 microlitters))Working Concentration: Westernblot 0.5 – 1 micrograms/mL

    [Recommended preparations]

    Frozen mouse brain tissue or cultured cells should be perfusion-fixed with 4% paraformaldehyde-PBS. Then the tissue section is prepared. After those preparation, the tissue section and cultured cells are immunostained with anti Iba1 antibody.

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      298-65701?? LabAssay? Glucose?? 1000tests???? –

      300-93523?? Phos-tag (TM) Acrylamide AAL-107????? 2mg????? –

      301-93531?? Phos-tag (TM) Biotin BTL-104 10mg??? –

      301-99413?? Phos-tagTM Biotin BTL-104 1mM Aqueous Solution[for Campaign]???? 0.1mL????? 753451-66-0

      Wako 019-11941 Active Carbon-impregnated Silica Gel 現(xiàn)貨

      302-93522?? Phos-tagTM Acrylamide AAL-107 5mM Aqueous Solution[for Campaign]????? 0.3mL??? 871839-54-2

      631-00651?? Cellmatrix Type I -A (Collagen, Type I, 3 mg/mL, pH 3.0)???? 20mL??? –

      637-00653?? Cellmatrix Type I -A (Collagen, Type I, 3 mg/mL, pH 3.0)???? 100mL?? –

      637-00653?? Cellmatrix Type I -A (Collagen, Type I, 3 mg/mL, pH 3.0)???? 100mL?? –

      638-01021?? Endotoxin Standad /JPSE? 10000un????? –

      986-10001?? Anti asialo GM1 (Rabbit), (014-09801)? 1mL????? 617-45-8

      Wako 019-11941 Active Carbon-impregnated Silica Gel 現(xiàn)貨