wako?125-05061賴氨酰肽鏈內(nèi)切酶

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wako?125-05061賴氨酰肽鏈內(nèi)切酶 Lysyl Endopeptidase?
【產(chǎn)品詳情】
賴氨酰肽鏈內(nèi)切酶
品牌：WAKO?和光
級別：質(zhì)譜級
賴氨酰肽鏈內(nèi)切酶Lysyl Endopeptidase 125-05061 wako
英文品名：Lysyl Endopeptidase?, Mass Spectrometry Grade
貨號：125-05061
規(guī)格：5×20 μg
應(yīng)用：蛋白質(zhì)組學(xué)。
用途：賴氨酰肽鏈內(nèi)切酶，蛋白測序、質(zhì)譜分析，多肽合成
賴氨酰肽鏈內(nèi)切酶Lysyl Endopeptidase 125-05061 wako
【實(shí)驗(yàn)原理】
賴氨酰肽鏈內(nèi)切酶是質(zhì)譜分析前處理時最常用的蛋白分解酶即賴氨酰肽鏈內(nèi)切酶，賴氨酰肽鏈內(nèi)切酶可以特異性切除賴氨酸基團(tuán)C?末端的多肽，賴氨酰肽鏈內(nèi)切酶可用于蛋白測序分析和Lys-X?化合物的酶合成。
若同時使用賴氨酰肽鏈內(nèi)切酶和胰酶，可更好地切斷賴氨酸基團(tuán)的多肽，增加多肽的數(shù)量。賴氨酰肽鏈內(nèi)切酶已按照使用習(xí)慣做成小包裝，是方便使用賴氨酰肽鏈內(nèi)切酶的冷凍干燥品。
【產(chǎn)品特點(diǎn)】
賴氨酰肽鏈內(nèi)切酶Lysyl Endopeptidase 125-05061 wako
1）賴氨酰肽鏈內(nèi)切酶用于蛋白質(zhì)譜分析：高特異性、高蛋白質(zhì)消化率；
2）賴氨酰肽鏈內(nèi)切酶能增加肽段數(shù)量、提高裂解效率；
3）賴氨酰肽鏈內(nèi)切酶獨(dú)立包裝，20 μg/一支
【結(jié)果】表明Lep?酶解的錯誤裂解率最低。
Tp?酶解時加入Lep?后，錯誤裂解率有所降低，同時，賴氨酰肽鏈內(nèi)切酶可以鑒定出更多的多肽。

Tp Lep Lep +Tp
裂解位點(diǎn) 精氨酸和賴氨酸的C?端 賴氨酸的C端 精氨酸和賴氨酸的C端
錯誤裂解率(?錯誤裂解所占比例) 多(8 %) 很少(0 %) 少(3 %)
鑒定出的多肽數(shù)量 17 19 22

賴氨酰肽鏈內(nèi)切酶Lysyl Endopeptidase 125-05061 wako
Lep+Tp?賴氨酰肽鏈內(nèi)切酶切后，可以在m/z=2000?時得到吸收峰，而單獨(dú)的Tp?酶切在m/z=2000?時沒有吸收峰。該結(jié)果表明Lep?可以提高測序覆蓋度。
賴氨酰肽鏈內(nèi)切酶Lysyl Endopeptidase 125-05061 wako

 

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