WAKO過氧化氫特異性熒光探針
| 品牌： | Wako(和光純藥) |
| 貨號： | 028-17811 |
| CAS號： | 728912-55-8 |
| 供應商： | 和光純藥株式會社 |
| 規(guī)格： | 1mg |
過氧化氫特異性熒光探針BES-H2O2-Ac / BES-H 2O2
? 比二氯熒光黃 (DCFH) 有更高的特異性
? 可動態(tài)監(jiān)測活細胞內的過氧化氫
? BES-H2O2-Ac 可穿透細胞膜，無需打孔
? 極易溶于水，可制成水溶液使用
? 可用于熒光顯微鏡、流式細胞儀
BES-So-AM：使用舉例
? 比二氯熒光黃 (DCFH) 有更高的特異性
? 可動態(tài)監(jiān)測活細胞內的過氧化氫
? BES-H2O2-Ac 可穿透細胞膜，無需打孔
? 極易溶于水，可制成水溶液使用
? 可用于熒光顯微鏡、流式細胞儀
BES-So-AM：使用舉例
圖1）是Jurkat T細胞在含33μM BES-So-AM的培養(yǎng)基中37°C培養(yǎng)1小時，使探針進入細胞，然后添加4mM丁酸（O2?-誘導劑），37°C培養(yǎng)1小時；圖2）是Jurkat T細胞在含33μM BES-So-AM的培養(yǎng)基中37°C培養(yǎng)1小時，使探針進入細胞，然后不添加丁酸，37°C再培養(yǎng)1小時；圖3）是Jurkat T細胞在含33μM BES-So-AM的培養(yǎng)基中37°C培養(yǎng)1小時，然后添加5mM的丁酸，繼續(xù)培養(yǎng)一個小時。參考文獻
1) Maeda, H. et al.: Angew. Chem. Int. Ed., 43, 239 (2004).
2) Maeda, H. et al.: Chem. Pharm. Bull., 49, 294 (2001).1. Features
1) Maeda, H. et al.: Angew. Chem. Int. Ed., 43, 239 (2004).
2) Maeda, H. et al.: Chem. Pharm. Bull., 49, 294 (2001).1. Features
?Much higher selectivity compared to conventionally-used DCFH probes?Responds specifically to hydrogen peroxide, enabling detection of intracellular hydrogen peroxide activity?BES-H2O2-Ac is cell membrane permeable?Superior water solubility allows use with aqueous solutions?Can be used in flow cytometry
2. Application
| H2O2-produced stimulation | No H2O2-produced stimulation | ||
| Fluorescent images |  |
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| Phase-contrast images | |||
| Fluorescent images of Jurkat T cells with BES- H2O2 -Ac and the same-fi eld phase-contrast images Jurkat T cells were cultured in a medium with 50μM BES-H2O2-Ac for 1 hour. Then, one group of them was cultured in a medium with 5 mM butyric acid (H2O2-produced stimulation), whereas the other in a medium without butyric acid (No H2O2– produced stimulation), each for 1 hour. (Courtesy: Professor Hatsuo Maeda, PhD., School of Pharmacy, Hyogo Univ. of Health Sciences) |
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3. References 參開文獻
- “Fluorescent probe for hydrogen peroxide based on a non-oxidative mechanism”, Maeda, H. et al.: Angew. Chem. Int. Ed., 43, 2389-91 (2004).
- “Hydrogen peroxide-induced deacetylation of acetyl resorufin as a novel indicator reaction for fluorometric detection of glucose using only glucose oxidase”, Maeda, H. et al.: Chem. Pharm. Bull., 49, 294-8 (2001).
4. Products List
< All products are for Cellbiology; ? Keep at room temperature >
| Cell Permeability | Product Name | Package Size | Wako Catalog No. | |
|---|---|---|---|---|
| Thiol/Selenol selective probe |
x
|
BES-Thio |
1 mg
|
025-15481
|
| H2O2 Probes |
o
<Detection of cell-derived H2O2 |
BES-H2O2-Ac |
1 mg
|
028-17811
|
|
x
|
BES-H2O2 ? (Cell-impermeant) |
1 mg
|
021-16201
|
|
| Superoxide selective probe |
o
<Detection of cell-derived superoxide> |
BES-So-AM |
1 mg
|
021-17801
|
|
x
|
BES-So ? (Cell-impermeant) |
1 mg
|
028-16211
|
說明書下載：
http://www.wako-chem.co.jp/english/labchem/product/life/BES-H2O2/pdf/WPUb1_p15-7.pdf