Lumiprobe 水溶性Cy染料
磺化和非磺化染料都可用于標(biāo)記生物分子例如DNA和蛋白質(zhì)。染料之間的差異是它們的溶解性：磺基染料是水溶性的，并且它們不使用有機(jī)共溶劑用于在水性環(huán)境中的標(biāo)記。它們不易于在水中聚集。
There are two varieties of cyanine dyes: non-sulfonated cyanines, and sulfonated cyanines. For many applications they are interchangeable, because their spectral properties are nearly identical. Both sulfonated and non-sulfonated dyes can be used for the labeling of biomolecules such as DNA and proteins. The difference between the dyes is their solubility: sulfo- dyes are water-soluble, and they do not use of organic co-solvent for the labeling in aqueous environment. They are less prone to aggregation in water.
- Sulfo-Cyanine3 carboxylic acid
- Sulfo-Cyanine5 carboxylic acid
- Sulfo-Cyanine5.5 carboxylic acid
- Sulfo-Cyanine7 carboxylic acid
- Sulfo-Cyanine7.5 carboxylic acid
磺酸基-Cy5羧酸
無活性的磺酸基-Cy5羧酸，水溶性染料。此染料是高水溶性染料
用于分子標(biāo)記應(yīng)該選擇具有活性的磺酸基-Cy5 NHS酯
分子結(jié)構(gòu)：
一般性質(zhì)：
外觀：深藍(lán)色粉末
分子量：664.76
分子式：C32H37N2NaO8S2
溶解性：易溶于水，DMSO,DMF，在多數(shù)有機(jī)溶劑難溶
質(zhì)控：NMR 1H（95%）和13C,TLC
光譜性質(zhì)：
最大激發(fā)波長：646nm
在最大激發(fā)波長下的消光系數(shù)：271000Lmol-1cm-1
最大發(fā)射波長：662nm
熒光量子產(chǎn)率：0.2
General properties
| Appearance: | dark blue powder |
| Molecular weight: | 664.76 |
| CAS number: | 1121756-16-8, 1144162-77-5 |
| Molecular formula: | C32H37N2NaO8S2 |
| Solubility: | Good in water, DMF, DMSO. Low in non-polar organic solvents. |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| Cat.?# | Quantity | Lead?time |
| 13390 | 1?mg | in?stock |
| 23390 | 5?mg | in?stock |
| 43390 | 25?mg | in?stock |
| 53390 | 50?mg | in?stock |
| 63390 | 100?mg | in?stock |
?磺酸基-Cy7羧酸
磺酸基-Cy7羧酸是無活性易溶于水的近紅外染料；
此試劑不能用于標(biāo)記分子，它有較高的水溶性，可改善近紅外光譜范圍的量子產(chǎn)率,并具有很高的消光系數(shù)。
分子結(jié)構(gòu)：
一般性質(zhì)：
外觀：深綠色粉末
分子量：730.87
分子式：C37h33N2NaO8S2
溶解性：易溶于水，DMF,DMSO
質(zhì)控：HPLC（95%），NMR 1H 和13C
光譜性質(zhì)：
最大激發(fā)波長：740nm
在最大激發(fā)波長下的消光系數(shù)：240600Lmol-1cm-1
最大發(fā)射波長：773nm
General properties
| Appearance: | dark green powder |
| Molecular weight: | 730.87 |
| Molecular formula: | C37H43N2NaO8S2 |
| Solubility: | Good in water, DMF, DMSO. Low in non-polar organic solvents. |
| Quality control: | NMR 1H, HPLC (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
Sulfo-Cyanine7 carboxylic acid is non-reactive water soluble near infrared dye.
The reagent is useful as a fluorescent marker in NIR range when attachment to other molecules is not desired. It has high hydrophilicity and aqueous solubility, improved quantum yield in NIR range, and very high molar extinction coefficient.
This reagent contains free carboxylic acid function. Pre-activated activated ester – sulfo-Cyanine7 NHS ester is also available.
| 15390 | 1?mg | in?stock |
| 25390 | 5?mg | in?stock |
| 45390 | 25?mg | in?stock |
| 55390 | 50?mg | in?stock |
| 65390 | 100?mg | in?stock |
磺基-Cy3 NHS酯：
此染料可溶于水，氨基活性Cy3染料。在水溶液中有效標(biāo)記蛋白和多肽，無需共溶劑，是低溶解性蛋白和易于降解蛋白的理想選擇。
此染料是磺化，親水性染料?？梢源鍭lexa Fluor 546和DyLight 549
分子結(jié)構(gòu)：
一般性質(zhì)：
外觀：深紅色晶體
分子量：735.8
分子式：C34H38N3NaO10S2
溶解性：極易溶于水，易溶于有機(jī)溶劑（DMF,DMSO）
質(zhì)控：NMR 1H（95%）和13C,TLC，功能性測試
光譜性質(zhì)：
最大激發(fā)波長：548nm
最大激發(fā)波長下的消光系數(shù)：162000Lmol-1cm-1
最大發(fā)射波長：563nm
熒光量子產(chǎn)率：0.1
General properties
| Appearance: | dark red crystals |
| Molecular weight: | 735.80 |
| Molecular formula: | C34H38N3NaO10S2 |
| Solubility: | very high in water, good in polar organic solvents (DMF, DMSO) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| 11320 | 1?mg | in?stock |
| 21320 | 5?mg | in?stock |
| 41320 | 25?mg | in?stock |
| 51320 | 50?mg | in?stock |
| 61320 | 100?mg | in?stock |
磺基-Cy5 NHS酯：
水溶性Cy5 NHS酯，用于標(biāo)記生物分子的氨基，無需任何共溶劑，因此可以用于標(biāo)記在有機(jī)溶劑中易降解的蛋白和溶解性低的蛋白
Cy5 是最常用的熒光團(tuán)之一，與多種設(shè)備兼容，比如平板讀數(shù)儀，顯微鏡和成像儀
此染料是高水溶性，可以代替多種用途的Alexa Fluor 647,DyLight 649
分子結(jié)構(gòu)：
一般性質(zhì)：
外觀：深藍(lán)色粉末
分子量：761,84
分子式：C36H40N3NaO10S2
溶解性：極易溶于水，易溶于有機(jī)溶劑DMF和DMSO
質(zhì)量控制：NMR 1H （95%）和13C,TLC,功能性測試
光譜性質(zhì)：
最大激發(fā)波長：646nm
最大激發(fā)波長下的消光系數(shù)：271000Lmol-1cm-1
最大發(fā)射波長：662nm
熒光量子產(chǎn)率：0.2
General properties
| Appearance: | dark blue powder |
| Molecular weight: | 777.95 |
| Molecular formula: | C36H40N3NaO10S2 |
| Solubility: | very good in water, good in DMF and DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| 13320 | 1?mg | in?stock |
| 23320 | 5?mg | in?stock |
| 43320 | 25?mg | in?stock |
| 53320 | 50?mg | in?stock |
| 63320 | 100?mg | in?stock |
磺基-Cy7 NHS酯：
一般性質(zhì)：
外觀：深綠色粉末
分子量：827.94
分子式：C41H46N3NaO10S2
溶解性：易溶于水,DMF,DMSO
質(zhì)控：NMR 1H(95%)13C,TLC,功能測試
光譜性質(zhì)：
最大激發(fā)波長：740nm
在最大激發(fā)波長下的消光系數(shù)：240600Lmol-1cm-1
最大發(fā)射波長：773nm
General properties
| Appearance: | dark green powder |
| Mass spec M+ increment: | 868.2 |
| Molecular weight: | 869.10 |
| Molecular formula: | C43H49KN4O9S2 |
| IUPAC name: | 1-[2-(6-{2-[(E)-2-[(E)-3-{(E)-2-[1-Methyl-3,3-dimethyl-5-(oxysulfonyl)-2-indolinylidene]ethylidene}-1-cyclohexen-1-yl]ethenyl]-3,3-dimethyl-5-(oxysulfonyl)-3H-indol-1-yl}hexanoylamino)ethyl]-1H-pyrrole-2,5-dione, potassium salt |
| Solubility: | Good in water, DMF, DMSO. Low in non-polar organic solvents. |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| 15380 | 1?mg | in?stock |
| 25380 | 5?mg | 5?days |
| 45380 | 25?mg | in?stock |
| 55380 | 50?mg | in?stock |
Sulfo-Cyanine5 maleimide ? 磺基-cy5-馬來酰亞胺
水溶的親水的sulfo-Cyanine5 maleimide（Cy5? maleimide的類似物）。作為Cy5? maleimide的完美的替代物，我們推薦該產(chǎn)品用于蛋白、包括抗體的標(biāo)記。通過凝膠過濾、離心柱純化、透析、電泳或色譜層析方法就可以很容易的將標(biāo)記蛋白和未標(biāo)記的染料進(jìn)行分類。
沒有磺化基團(tuán)的類似物：Cyanine5 maleimide。
磺酸基類熒光染料：
- Sulfo-Cyanine3 NHS ester
- Sulfo-Cyanine5 NHS ester
- Sulfo-Cyanine5 bis-NHS ester
- Sulfo-Cyanine5.5 NHS ester
- Sulfo-Cyanine7 NHS ester
- Sulfo-Cyanine3 maleimide
- Sulfo-Cyanine5 maleimide
- Sulfo-Cyanine7 maleimide
- Sulfo-Cyanine3 azide
- Sulfo-Cyanine5 azide
- Sulfo-Cyanine5.5 azide
- Sulfo-Cyanine7 azide
- Sulfo-Cyanine3 alkyne
- Sulfo-Cyanine5 alkyne
- Sulfo-Cyanine5.5 alkyne
- Sulfo-Cyanine7 alkyne
- Sulfo-Cyanine3 amine
- Sulfo-Cyanine5 amine
- Sulfo-Cyanine5.5 amine
- Sulfo-Cyanine7 amine